##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1545727_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	443174
Sequences flagged as poor quality	0
Sequence length	51
%GC	46
>>END_MODULE
>>Per base sequence quality	fail
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.005322965697445	31.0	31.0	33.0	30.0	33.0
2	31.17279668933647	31.0	31.0	33.0	30.0	34.0
3	31.108239653048237	31.0	31.0	34.0	28.0	34.0
4	35.08695455960864	35.0	35.0	37.0	32.0	37.0
5	32.83046613745391	35.0	35.0	37.0	28.0	37.0
6	34.00239860641644	35.0	35.0	37.0	28.0	37.0
7	15.521346017591284	0.0	0.0	35.0	0.0	37.0
8	25.01229313994052	17.0	17.0	35.0	17.0	37.0
9	32.69570191392094	32.0	32.0	37.0	27.0	39.0
10	35.48413038671041	37.0	34.0	37.0	32.0	39.0
11	36.43986109293415	37.0	35.0	39.0	32.0	39.0
12	36.372325542563416	37.0	35.0	39.0	32.0	39.0
13	36.146870078118305	38.0	35.0	39.0	32.0	39.0
14	37.16152572127426	39.0	36.0	40.0	32.0	41.0
15	37.46029776115025	39.0	36.0	41.0	32.0	41.0
16	37.47674051275571	39.0	36.0	41.0	32.0	41.0
17	37.430158357665384	39.0	36.0	41.0	32.0	41.0
18	37.35628443906908	39.0	36.0	40.0	32.0	41.0
19	37.315363717185576	39.0	36.0	40.0	32.0	41.0
20	37.43130463429714	39.0	35.0	41.0	32.0	41.0
21	37.295969528898354	39.0	35.0	41.0	32.0	41.0
22	37.3408954496428	39.0	35.0	41.0	32.0	41.0
23	37.33153343833347	39.0	35.0	41.0	32.0	41.0
24	37.15704215500007	39.0	35.0	40.0	32.0	41.0
25	36.90669353346541	39.0	35.0	40.0	31.0	41.0
26	36.783200277994645	38.0	35.0	40.0	31.0	41.0
27	36.71699152026066	38.0	35.0	40.0	31.0	41.0
28	36.63057850866702	38.0	35.0	40.0	31.0	41.0
29	36.605315293767234	38.0	35.0	40.0	31.0	41.0
30	36.32914837061741	38.0	35.0	40.0	30.0	41.0
31	36.195322830310445	38.0	35.0	40.0	30.0	41.0
32	36.09825486152166	38.0	35.0	40.0	30.0	41.0
33	35.903441988925344	38.0	35.0	40.0	29.0	41.0
34	35.719498436280105	38.0	35.0	40.0	28.0	41.0
35	35.512730891252644	38.0	34.0	40.0	27.0	41.0
36	35.33557699684548	38.0	34.0	40.0	26.0	41.0
37	35.2289710136425	38.0	34.0	40.0	26.0	41.0
38	35.077470699995935	38.0	34.0	40.0	25.0	41.0
39	34.970661636287325	38.0	34.0	40.0	25.0	41.0
40	34.811586419780944	38.0	34.0	40.0	24.0	41.0
41	34.66702920297671	38.0	34.0	40.0	24.0	41.0
42	34.62587155383664	38.0	33.0	40.0	24.0	41.0
43	34.57473588252019	38.0	33.0	40.0	23.0	41.0
44	34.35745779310158	37.0	33.0	40.0	23.0	41.0
45	34.260279258259736	37.0	33.0	40.0	23.0	41.0
46	34.23655268585251	37.0	33.0	40.0	23.0	41.0
47	34.14214281523736	37.0	33.0	40.0	23.0	41.0
48	34.073979971749246	37.0	33.0	40.0	23.0	41.0
49	33.984529778371474	36.0	33.0	40.0	23.0	41.0
50	33.865788606732345	36.0	33.0	40.0	23.0	41.0
51	33.69854052809957	36.0	33.0	40.0	23.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
13	4.0
14	5.0
15	23.0
16	48.0
17	132.0
18	261.0
19	503.0
20	820.0
21	1427.0
22	2194.0
23	3338.0
24	4625.0
25	6348.0
26	7544.0
27	8161.0
28	9090.0
29	10572.0
30	12627.0
31	16565.0
32	21516.0
33	31477.0
34	39954.0
35	44358.0
36	55016.0
37	81464.0
38	69919.0
39	15183.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	34.909764562000476	23.491675955719423	27.206469693619212	14.392089788660886
2	34.451028264293484	23.076263499212498	29.612748040273118	12.859960196220898
3	31.777811875245387	22.850392847955884	32.0932635939834	13.278531682815329
4	27.47115128595089	26.992332582687613	29.733693763623315	15.802822367738178
5	21.364746126803468	36.172699661983785	28.098895693339408	14.36365851787334
6	20.91842030444024	41.08837612314802	28.274673153208447	9.718530419203294
7	38.7062417921629	56.10437435409117	4.371646351094604	0.8177375026513288
8	87.38441334554825	3.327812552180408	7.506983712943449	1.7807903893278936
9	82.39314580729014	5.001195918533127	9.449335926746604	3.1563223474301285
10	46.77846624576352	24.734754295152694	15.436826167600085	13.049953291483707
11	39.679674349126984	23.33643219141917	21.538267136610003	15.44562632284385
12	34.976329838844336	22.115467062598437	27.133135066587844	15.775068031969383
13	23.387653607838004	32.44572109374648	27.868060851945287	16.298564446470234
14	18.463853926448753	35.01310997486314	30.798512548118794	15.724523550569302
15	17.116978884140316	23.743270137688583	42.56003285391291	16.579718124258193
16	19.970485633182452	19.473164039406644	40.72373379304743	19.832616534363478
17	20.54227007902088	19.397121672300273	29.59943498490435	30.461173263774498
18	23.411571978500547	22.708913429036905	32.92408850699725	20.955426085465305
19	28.853452594240636	23.96372530879519	26.84995058374363	20.33287151322054
20	30.29442160415548	24.07812732696413	25.49608054624143	20.131370522638964
21	25.69735589181676	25.74880295324184	29.182668658359923	19.37117249658148
22	23.911150022338855	22.674615388086846	27.813680405438944	25.600554184135348
23	22.089743531885897	27.98359109514547	26.952393416581298	22.974271956387334
24	21.712465081435283	25.15783868187213	33.75604164504236	19.373654591650233
25	21.965638778448195	24.05037299119533	32.42857207327145	21.555416157085027
26	20.170858398732776	29.266157310672558	28.052638467058085	22.51034582353658
27	20.163637758532765	28.773348617021753	30.195363446411566	20.867650178033912
28	18.93048779937451	26.54126821519313	35.07854702667576	19.449696958756608
29	19.77755915283839	25.58227693862907	34.16536168638052	20.47480222215202
30	22.697405533718136	26.69402988442463	30.863498309918903	19.745066271938335
31	29.230505399684997	24.270828162301942	26.38828090095538	20.110385537057677
32	29.281952461110084	25.44147445472884	26.91132602544373	18.365247058717344
33	28.17200467536453	24.717153984665167	26.524796129736856	20.586045210233454
34	21.976695383754464	26.976537432250087	28.438942717758714	22.607824466236735
35	22.685671993393115	25.502398606416442	30.12857252456146	21.683356875628988
36	29.25013651522878	25.26186102975355	25.73661812290432	19.751384332113346
37	22.500643088267815	30.565872546674672	26.414681366686676	20.518802998370845
38	23.085514944468763	30.983090163231598	24.137923253620475	21.793471638679165
39	22.588870285711707	28.80493891789681	25.39002739330376	23.216163403087727
40	24.535735399639872	25.338805976884927	26.864843154156155	23.26061546931905
41	20.38702631472063	24.75641621575273	27.346143952488188	27.510413517038458
42	22.582326580530445	26.218370211248853	25.615672399554125	25.583630808666573
43	23.036549978112433	25.77249567889813	26.31562320894276	24.87533113404667
44	21.51976424609747	27.39646278888202	29.302034866666364	21.781738098354143
45	20.842603582340118	31.36104554870096	25.612513369466622	22.1838374994923
46	23.531163831813238	29.21651540929748	27.111021855975302	20.141298902913977
47	22.423698141136438	27.306656076394376	27.178264067837915	23.09138171463127
48	22.803909976668308	26.471995198274268	29.185827688447425	21.538267136610003
49	21.929986867460634	25.86049723133577	30.261703078249173	21.947812822954415
50	20.74422235961496	30.317663039799264	27.380667638444496	21.55744696214128
51	21.399721102772272	30.680951499862356	25.812209199998193	22.107118197367175
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	298.0
1	495.5
2	693.0
3	2832.5
4	4972.0
5	3577.5
6	2183.0
7	2230.0
8	2277.0
9	2262.5
10	2248.0
11	2215.5
12	2183.0
13	2101.0
14	2019.0
15	1943.5
16	1868.0
17	1851.0
18	1834.0
19	1911.5
20	1989.0
21	2038.0
22	2087.0
23	2280.5
24	2474.0
25	2710.0
26	3262.0
27	3578.0
28	4348.0
29	5118.0
30	6231.5
31	7345.0
32	8776.0
33	10207.0
34	11065.5
35	11924.0
36	12990.0
37	14056.0
38	16936.5
39	19817.0
40	24677.0
41	29537.0
42	31939.5
43	34342.0
44	34884.5
45	35427.0
46	34853.0
47	34279.0
48	33373.5
49	32468.0
50	31534.5
51	30601.0
52	28675.0
53	26749.0
54	25439.5
55	24130.0
56	22847.5
57	21565.0
58	20409.5
59	19254.0
60	17327.5
61	15401.0
62	13663.0
63	11925.0
64	10183.5
65	8442.0
66	7072.0
67	5702.0
68	4871.0
69	4040.0
70	3532.0
71	3024.0
72	2523.0
73	2022.0
74	1539.0
75	792.5
76	529.0
77	404.0
78	279.0
79	215.0
80	151.0
81	109.5
82	68.0
83	48.5
84	29.0
85	26.0
86	23.0
87	15.0
88	7.0
89	6.0
90	5.0
91	4.0
92	3.0
93	1.5
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	443174.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	36.659555205402114
#Duplication Level	Percentage of deduplicated	Percentage of total
1	66.12837388689906	24.24236773150247
2	9.502312637910261	6.9670110945692265
3	5.284278257329584	5.811578714858397
4	3.7473862785006236	5.495100566106399
5	2.7012459975039134	4.951323838443309
6	2.2104005104581947	4.8619379723514715
7	1.778792639611441	4.5646822879558995
8	1.44871221195458	4.24873162487113
9	1.1889551072571634	3.922790885211489
>10	5.958538973453669	32.55807079895127
>50	0.033797499417863745	0.8317077788681926
>100	0.0153624997353926	0.8145870336910572
>500	6.14499989415704E-4	0.15814161992564216
>1k	0.001228999978831408	0.5719680526940248
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CGTTTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1305	0.2944667331567285	No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1234	0.27844593771295245	No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATAC	702	0.1584027943877574	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0060924151687599005	0.0
2	0.0	0.0	0.0	0.025272240700041068	0.0
3	0.0	0.0	0.0	0.0331698159188039	0.0
4	0.0	0.0	0.0	0.047385451312577	0.0
5	0.0	0.0	0.0	0.08912977746889483	0.0
6	0.0	0.0	0.0	0.13696651879397256	0.0
7	0.0	0.0	0.0	0.16787988465027281	0.0
8	0.0	0.0	0.0	0.3756989354068605	0.0
9	0.0	0.0	0.0	0.46866467798201156	0.0
10	0.0	0.0	0.0	0.618492962132255	0.0
11	0.0	0.0	0.0	0.7536543208762246	0.0
12	0.0	0.0	0.0	0.8633177939139028	0.0
13	0.0	0.0	0.0	0.9136366303077347	0.0
14	0.0	0.0	0.0	0.9371037109577728	0.0
15	0.0	0.0	0.0	0.968017076814073	0.0
16	0.0	0.0	0.0	1.0330028386141785	0.0
17	0.0	0.0	0.0	1.1137837508518098	0.0
18	0.0	0.0	0.0	1.2277344790082452	0.0
19	0.0	0.0	0.0	1.275119930320822	0.0
20	0.0	0.0	0.0	1.3371723070396728	0.0
21	0.0	0.0	0.0	1.4087017740210392	0.0
22	0.0	0.0	0.0	1.4858723661586646	0.0
23	0.0	0.0	0.0	1.56304295829629	0.0
24	0.0	0.0	0.0	1.6302851701588992	0.0
25	0.0	0.0	0.0	1.6842143266527367	0.0
26	0.0	0.0	0.0	1.7435589632965833	0.0
27	0.0	0.0	0.0	1.8094473051216904	0.0
28	0.0	0.0	0.0	1.8784946770343025	0.0
29	0.0	0.0	0.0	1.9468651139281636	0.0
30	0.0	0.0	0.0	2.048631011747079	0.0
31	0.0	0.0	0.0	2.1375351442097235	0.0
32	0.0	0.0	0.0	2.2115467062598437	0.0
33	0.0	0.0	0.0	2.2945840685599785	0.0
34	0.0	0.0	0.0	2.390031906203884	0.0
35	0.0	0.0	0.0	2.5087211794915767	0.0
36	0.0	0.0	0.0	2.5996561170104746	0.0
37	0.0	0.0	0.0	2.688560249473119	0.0
38	0.0	0.0	0.0	2.7923569523482876	0.0
39	0.0	0.0	0.0	2.96610360716107	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TTTACGG	50	2.1827873E-11	45.000004	2
AATAGGC	50	2.1827873E-11	45.000004	4
ATCGAAG	25	3.8869126E-5	45.000004	2
TCGCAAC	40	6.7993824E-9	45.0	16
AATCTCG	20	7.0285896E-4	45.0	18
CTCGAGC	20	7.0285896E-4	45.0	36
CGACGCT	30	2.1624728E-6	44.999996	12
CGTACAT	30	2.1624728E-6	44.999996	35
CGTAAAG	30	2.1624728E-6	44.999996	2
CGTTTTA	730	0.0	43.150684	1
AGGATGC	85	0.0	42.35294	7
AGGCGAT	375	0.0	41.999996	7
CGTTTTT	1045	0.0	41.339714	1
CGACGGT	100	0.0	40.500004	28
CGGTCTA	100	0.0	40.500004	31
ATAACGC	50	1.0786607E-9	40.500004	11
GGTCTAA	100	0.0	40.500004	32
CGACGTC	45	1.9237632E-8	40.0	18
TAACGGG	45	1.9237632E-8	40.0	3
CGTCAGA	45	1.9237632E-8	40.0	31
>>END_MODULE