FastQCFastQC Report
Thu 26 May 2016
SRR1545726_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1545726_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences1062141
Sequences flagged as poor quality0
Sequence length50
%GC43

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT41880.39429793219544296No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATA30700.2890388375931256No Hit
CGTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT17190.16184291916045043No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT16390.15431096248049928No Hit

[WARN]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AACCGGT207.8562106E-444.00000427
TCGTTGA207.8562106E-444.00000421
TCGTTCC207.8562106E-444.00000413
AGATCCG207.8562106E-444.00000434
CCCTCTA207.8562106E-444.00000421
CGTGCCG207.8562106E-444.00000441
CGTGCAA207.8562106E-444.00000438
CGCATTG207.8562106E-444.00000427
CGCATAT207.8562106E-444.00000434
GCCGGAT408.310963E-944.00000419
TATATCG207.8562106E-444.0000041
GGTCATC207.8562106E-444.0000048
CGCGATC207.8562106E-444.00000428
ATCGTTG207.8562106E-444.00000420
CTGCGTA207.8562106E-444.00000415
GCCGATG408.310963E-944.0000049
CCGTAGA207.8562106E-444.0000041
TACGCAA207.8562106E-444.0000041
GCCGAAC207.8562106E-444.00000437
TACGATG207.8562106E-444.0000041
Produced by FastQC (version 0.11.5)