##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1545725_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	404185
Sequences flagged as poor quality	0
Sequence length	51
%GC	43
>>END_MODULE
>>Per base sequence quality	fail
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.059774608162105	31.0	31.0	33.0	30.0	34.0
2	31.205057090193847	31.0	31.0	33.0	30.0	34.0
3	31.11302250207207	31.0	31.0	34.0	28.0	34.0
4	35.05742667342925	35.0	35.0	37.0	32.0	37.0
5	32.80399569504064	35.0	35.0	37.0	28.0	37.0
6	33.99908457760679	35.0	35.0	37.0	28.0	37.0
7	15.018917080049977	0.0	0.0	35.0	0.0	37.0
8	24.791323280181103	17.0	17.0	35.0	17.0	37.0
9	32.80907505226567	32.0	32.0	37.0	27.0	39.0
10	35.64684241127207	37.0	35.0	37.0	32.0	39.0
11	36.538174350854185	37.0	35.0	39.0	32.0	39.0
12	36.20965894330566	37.0	35.0	39.0	32.0	39.0
13	35.69239828296448	37.0	35.0	39.0	31.0	39.0
14	36.69229189603771	39.0	35.0	40.0	31.0	41.0
15	37.20498286675656	39.0	35.0	41.0	32.0	41.0
16	37.34615584447716	39.0	35.0	41.0	32.0	41.0
17	37.31264643665648	39.0	35.0	41.0	32.0	41.0
18	37.208162103987036	39.0	35.0	40.0	32.0	41.0
19	37.09951631060034	39.0	35.0	40.0	32.0	41.0
20	37.17174066331012	39.0	35.0	40.0	32.0	41.0
21	36.99053898586043	39.0	35.0	40.0	32.0	41.0
22	37.04131276519416	39.0	35.0	40.0	32.0	41.0
23	37.032866138030855	38.0	35.0	40.0	32.0	41.0
24	36.85258730531811	38.0	35.0	40.0	32.0	41.0
25	36.60455731904945	38.0	35.0	40.0	31.0	41.0
26	36.45423011739673	38.0	35.0	40.0	31.0	41.0
27	36.3341737075844	38.0	35.0	40.0	31.0	41.0
28	36.30964780979007	38.0	35.0	40.0	31.0	41.0
29	36.34300877073617	38.0	35.0	40.0	31.0	41.0
30	36.08490418991303	38.0	35.0	40.0	30.0	41.0
31	35.907633880525005	38.0	34.0	40.0	30.0	41.0
32	35.67445600405755	38.0	34.0	40.0	29.0	41.0
33	35.40155374395388	38.0	34.0	40.0	28.0	41.0
34	35.21255365735987	38.0	34.0	40.0	27.0	41.0
35	34.97043927904301	38.0	34.0	40.0	25.0	41.0
36	34.766911191657286	38.0	34.0	40.0	24.0	41.0
37	34.561032695424124	37.0	33.0	40.0	23.0	41.0
38	34.51572423518933	37.0	33.0	40.0	23.0	41.0
39	34.418738943800484	37.0	33.0	40.0	23.0	41.0
40	34.24318566992837	37.0	33.0	40.0	23.0	41.0
41	34.16711901728169	37.0	33.0	40.0	23.0	41.0
42	34.12392097678043	37.0	33.0	40.0	23.0	41.0
43	34.00151663223524	37.0	33.0	40.0	22.0	41.0
44	33.74086371339857	36.0	33.0	40.0	21.0	41.0
45	33.648861288766284	36.0	33.0	40.0	20.0	41.0
46	33.67866942118089	36.0	33.0	40.0	20.0	41.0
47	33.67423085963111	36.0	33.0	40.0	21.0	41.0
48	33.68727686579165	36.0	33.0	40.0	20.0	41.0
49	33.61251159741207	36.0	33.0	40.0	20.0	41.0
50	33.44528866731818	36.0	33.0	40.0	20.0	41.0
51	33.19549463735665	35.0	32.0	40.0	19.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
12	1.0
13	4.0
14	5.0
15	15.0
16	50.0
17	135.0
18	327.0
19	583.0
20	1006.0
21	1639.0
22	2486.0
23	3715.0
24	5238.0
25	6999.0
26	8153.0
27	8633.0
28	8931.0
29	9821.0
30	11839.0
31	15462.0
32	20555.0
33	32259.0
34	40688.0
35	40615.0
36	43263.0
37	67844.0
38	63471.0
39	10448.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	29.92367356532281	23.7821789527073	29.825946039561092	16.468201442408798
2	32.90201269220778	24.100350087212536	30.550614198943553	12.447023021636133
3	28.171010799510128	22.567636107228125	36.38111260932494	12.880240483936811
4	26.346846122443928	27.94166037829212	31.101846926531167	14.609646572732785
5	19.573957469970434	36.862822717320036	29.68294221705407	13.880277595655455
6	19.398790157972215	40.434207108131176	29.738609795019606	10.428392938877
7	36.70645867610129	57.71144401697242	4.879943590187661	0.7021537167386221
8	87.71874265497235	3.0018432153593033	7.871642935784356	1.4077711938839887
9	84.54222695053008	3.9674901344681275	9.34473075448124	2.1455521605205536
10	59.12614273166991	18.366094733847124	13.706099929487733	8.801662604995238
11	52.37947969370461	17.59342875168549	19.262713856278683	10.76437769833121
12	45.194156141370904	20.392147160334993	22.870467731360637	11.543228966933459
13	19.93641525539048	45.81342701980529	23.510521172235485	10.73963655256875
14	12.867127676682708	47.627447827108874	28.29916993455967	11.20625456164875
15	10.898969531278993	21.52009599564556	56.23934584410604	11.341588628969408
16	12.697650828209856	14.68213812981679	53.84217623118127	18.77803481079209
17	13.976273241213802	15.615374147976793	31.089228942192314	39.319123668617095
18	20.38200329057239	22.267526009129483	36.88236822247239	20.46810247782575
19	29.739352029392478	22.571842102007743	27.26350557294308	20.425300295656694
20	31.153308509717085	22.35659413387434	25.910164899736504	20.57993245667207
21	20.366416368742037	28.15121788290016	30.754976062941473	20.72738968541633
22	21.251654564122866	23.983571879213727	26.644976928881576	28.119796627781835
23	17.643158454668036	30.73642020361963	26.037334388955554	25.583086952756783
24	18.071922510731472	23.419226344372007	42.3974170243824	16.11143412051412
25	15.41967168499573	23.423184927694003	40.497544441283075	20.65959894602719
26	13.222905352746887	36.04166408946398	30.072862674270446	20.662567883518687
27	15.831611761940694	36.2489948909534	31.41803876937541	16.501354577730496
28	13.387681383524871	28.182886549476105	43.29626284993258	15.133169217066442
29	13.56829174759083	23.77797295792768	42.192560337469224	20.46117495701226
30	17.33760530450165	29.82322451352722	34.96418719150884	17.87498299046229
31	30.95414228632928	24.230488513923078	26.384700075460493	18.430669124287146
32	32.159530907876345	25.245370313099198	27.50992738473719	15.08517139428727
33	29.111174338483615	26.063312592006138	26.434182366985414	18.391330702524833
34	19.4871160483442	26.83103034501528	29.783391268849662	23.898462337790864
35	19.652881724952685	24.76316538218885	33.33745685762708	22.24649603523139
36	33.01310043668123	22.346202852654105	27.36148051016243	17.279216200502244
37	20.208815270235164	32.511597412076156	30.21834061135371	17.06124670633497
38	21.13685564778505	33.776364783453126	23.52858220864208	21.558197360119745
39	20.474287764266364	31.13673194205624	27.895889258631566	20.493091035045833
40	23.94200675433279	24.704281455274195	26.537352944814874	24.816358845578137
41	16.54786793176392	23.416999641253387	27.944381904325986	32.090750522656705
42	23.187649220035382	24.85124386110321	24.2789811596175	27.68212575924391
43	23.112188725459877	25.95123520170219	25.41039375533481	25.52618231750312
44	19.93171443769561	31.525910164899734	28.88998849536722	19.652386902037435
45	16.964261414946126	39.26345609065156	23.10204485569727	20.67023763870505
46	22.51617452404221	32.61649987010898	26.804309907591822	18.063015698256986
47	21.90135705184507	26.99803307891188	27.741999331989064	23.35861053725398
48	24.922003537983844	22.396674790009524	31.497210435815283	21.184111236191345
49	21.675717802491434	22.20121973848609	33.306530425424	22.816532033598477
50	19.232034835533234	34.1019582616871	26.23773766963148	20.428269233148185
51	18.535818993777603	35.10125313903287	23.776241077724308	22.586686789465222
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	387.0
1	439.5
2	492.0
3	2297.5
4	4103.0
5	3223.5
6	2344.0
7	2463.5
8	2583.0
9	2793.5
10	3004.0
11	3000.5
12	2997.0
13	2907.5
14	2818.0
15	2679.5
16	2541.0
17	2379.5
18	2218.0
19	2089.5
20	1961.0
21	1951.5
22	1942.0
23	2039.0
24	2136.0
25	2381.5
26	2929.0
27	3231.0
28	3503.0
29	3775.0
30	4532.5
31	5290.0
32	6326.5
33	7363.0
34	7939.0
35	8515.0
36	10607.0
37	12699.0
38	17908.0
39	23117.0
40	31184.0
41	39251.0
42	42601.5
43	45952.0
44	44202.5
45	42453.0
46	40412.5
47	38372.0
48	35555.0
49	32738.0
50	30061.5
51	27385.0
52	24330.0
53	21275.0
54	18853.0
55	16431.0
56	14363.0
57	12295.0
58	11024.0
59	9753.0
60	8402.0
61	7051.0
62	5970.5
63	4890.0
64	4198.5
65	3507.0
66	2923.0
67	2339.0
68	1985.5
69	1632.0
70	1355.0
71	1078.0
72	926.5
73	775.0
74	608.5
75	313.5
76	185.0
77	135.0
78	85.0
79	76.0
80	67.0
81	56.5
82	46.0
83	34.0
84	22.0
85	16.0
86	10.0
87	7.0
88	4.0
89	3.5
90	3.0
91	2.0
92	1.0
93	0.5
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	404185.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	29.458440993111196
#Duplication Level	Percentage of deduplicated	Percentage of total
1	72.92321212481998	21.4820414140714
2	6.53927766601215	3.8527385052357768
3	3.3368437564147433	2.9489464470472564
4	2.1941872138750456	2.5854933827110833
5	1.6075848356439195	2.367847151111838
6	1.3752698458227246	2.430798336166432
7	1.1023125229923412	2.2730685890176185
8	0.9780445037450612	2.3049333041768487
9	0.8227297276738547	2.181270162236282
>10	8.92492980762427	52.82823387042489
>50	0.17881760950674333	3.3035575718186148
>100	0.014271827988801111	0.5821660820527789
>500	0.0	0.0
>1k	0.0025185578803766664	0.8589051839291848
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATAC	1270	0.3142125511832453	No Hit
CGTTTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1193	0.2951618689461509	No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1010	0.24988557220084864	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.009649046847359502	0.0
2	0.0	0.0	0.0	0.04354441654193006	0.0
3	0.0	0.0	0.0	0.054183109219787966	0.0
4	0.0	0.0	0.0	0.07867684352462362	0.0
5	0.0	0.0	0.0	0.11776785382931083	0.0
6	0.0	0.0	0.0	0.17887848386258767	0.0
7	0.0	0.0	0.0	0.21796949416727487	0.0
8	0.0	0.0	0.0	0.6185286440615065	0.0
9	0.0	0.0	0.0	0.8169526330764377	0.0
10	0.0	0.0	0.0	1.1593700904288877	0.0
11	0.0	0.0	0.0	1.436965745883692	0.0
12	0.0	0.0	0.0	1.6917995472370326	0.0
13	0.0	0.0	0.0	1.7944753021512427	0.0
14	0.0	0.0	0.0	1.8239172656085703	0.0
15	0.0	0.0	0.0	1.8699357967267465	0.0
16	0.0	0.0	0.0	1.996610463030543	0.0
17	0.0	0.0	0.0	2.16039684797803	0.0
18	0.0	0.0	0.0	2.398159258755273	0.0
19	0.0	0.0	0.0	2.482773977262887	0.0
20	0.0	0.0	0.0	2.5753058624144884	0.0
21	0.0	0.0	0.0	2.6792186746168216	0.0
22	0.0	0.0	0.0	2.7818944295310315	0.0
23	0.0	0.0	0.0	2.9028786323094624	0.0
24	0.0	0.0	0.0	2.9949156945458144	0.0
25	0.0	0.0	0.0	3.0585004391553374	0.0
26	0.0	0.0	0.0	3.1364350483070873	0.0
27	0.0	0.0	0.0	3.208926605391096	0.0
28	0.0	0.0	0.0	3.2851293343394734	0.0
29	0.0	0.0	0.0	3.3677647611860904	0.0
30	0.0	0.0	0.0	3.4823162660662814	0.0
31	0.0	0.0	0.0	3.5966203594888477	0.0
32	0.0	0.0	0.0	3.6839566040303326	0.0
33	0.0	0.0	0.0	3.7618912131820825	0.0
34	0.0	0.0	0.0	3.8489800462659427	0.0
35	0.0	0.0	0.0	3.9778814156883606	0.0
36	0.0	0.0	0.0	4.074866707077205	0.0
37	0.0	0.0	0.0	4.176057993245667	0.0
38	0.0	0.0	0.0	4.273043284634511	0.0
39	0.0	0.0	0.0	4.374234570802974	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGACGGT	65	0.0	45.000004	28
CGGTCTA	60	0.0	45.000004	31
ATGCCTC	30	2.1619962E-6	45.000004	43
TTCGCGA	30	2.1619962E-6	45.000004	1
CCGCAGG	30	2.1619962E-6	45.000004	3
TCGCCAG	20	7.027852E-4	45.0	25
TTTAGCG	20	7.027852E-4	45.0	1
AGCGTAA	25	3.8863018E-5	45.0	1
AGTCCAC	20	7.027852E-4	45.0	16
TCCGCGT	20	7.027852E-4	45.0	29
TCACCGA	20	7.027852E-4	45.0	1
CGTATGG	25	3.8863018E-5	45.0	2
CGAGTGT	20	7.027852E-4	45.0	16
TTAGCCT	25	3.8863018E-5	45.0	28
CGTGCAG	25	3.8863018E-5	45.0	2
CGCATCG	20	7.027852E-4	45.0	21
CGACAAG	25	3.8863018E-5	45.0	2
CTGCGAA	35	1.2093187E-7	45.0	1
TCGTGAA	20	7.027852E-4	45.0	1
CGTAGTC	20	7.027852E-4	45.0	13
>>END_MODULE