FastQCFastQC Report
Thu 26 May 2016
SRR1545724_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1545724_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences1441008
Sequences flagged as poor quality0
Sequence length50
%GC46

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT65870.45711057815084993No Hit
CGTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT28960.20097043180884488No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT27810.19298990706505445No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATCACG207.857146E-444.00000419
CGTTTAG650.044.0000042
CGATACG302.528166E-644.00000410
CGTTTTA35150.043.561881
CGGTCTA3700.042.8108131
CGTTATT18900.042.370371
AGGCGAT10000.041.87
GCGATAT1400.040.8571439
ATAACGC1150.040.17391611
GTTTTAT41700.039.6211052
CGACGGT3950.039.54430428
AGGTACC4950.039.111117
TCGAATA452.3515895E-839.111111
CGTTTTT19100.038.5863841
CAAGGAC10950.038.3744285
TAACGGG3000.038.133333
TCTCACG4100.038.09756523
ATAGGCG1850.038.0540545
TCACGAC4050.038.02469325
AGGCGCG6400.037.8125047
Produced by FastQC (version 0.11.5)