##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1545724_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	1441008
Sequences flagged as poor quality	0
Sequence length	50
%GC	46
>>END_MODULE
>>Per base sequence quality	fail
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.474728800950444	31.0	31.0	33.0	30.0	34.0
2	31.656649373216528	31.0	31.0	34.0	30.0	34.0
3	31.71427431353608	31.0	31.0	34.0	30.0	34.0
4	35.632858388017276	37.0	35.0	37.0	33.0	37.0
5	27.151820114808523	35.0	26.0	37.0	0.0	37.0
6	31.35328048144077	35.0	27.0	37.0	17.0	37.0
7	14.97870032643816	0.0	0.0	32.0	0.0	37.0
8	25.069298019164364	17.0	17.0	35.0	17.0	37.0
9	33.101374870923685	32.0	32.0	37.0	28.0	39.0
10	35.91230860619788	37.0	35.0	39.0	32.0	39.0
11	36.907794405027595	38.0	37.0	39.0	34.0	39.0
12	37.060764409357894	39.0	37.0	39.0	34.0	39.0
13	36.7170910917913	39.0	35.0	39.0	33.0	39.0
14	38.04244320642217	40.0	37.0	41.0	33.0	41.0
15	38.26145517582137	40.0	37.0	41.0	33.0	41.0
16	38.2927291173956	40.0	37.0	41.0	34.0	41.0
17	38.30735082664357	40.0	37.0	41.0	34.0	41.0
18	38.27892003375415	40.0	37.0	41.0	34.0	41.0
19	38.22556085739982	40.0	37.0	41.0	34.0	41.0
20	38.25914915114975	40.0	37.0	41.0	34.0	41.0
21	38.15799634700154	40.0	37.0	41.0	34.0	41.0
22	38.07020988086118	40.0	36.0	41.0	34.0	41.0
23	37.96284614658628	40.0	36.0	41.0	34.0	41.0
24	37.80922867881372	40.0	35.0	41.0	33.0	41.0
25	37.63073973218747	39.0	35.0	41.0	33.0	41.0
26	37.38332472824578	39.0	35.0	40.0	33.0	41.0
27	37.195166161464755	39.0	35.0	40.0	33.0	41.0
28	37.203604004974295	39.0	35.0	40.0	32.0	41.0
29	37.089615047244706	39.0	35.0	40.0	32.0	41.0
30	36.87533240620454	39.0	35.0	40.0	32.0	41.0
31	36.71486417840845	39.0	35.0	40.0	31.0	41.0
32	36.53891026281603	39.0	35.0	40.0	31.0	41.0
33	36.328644948536024	38.0	35.0	40.0	30.0	41.0
34	36.07226052874099	38.0	35.0	40.0	30.0	41.0
35	35.86830399276062	38.0	35.0	40.0	29.0	41.0
36	35.677365427534056	38.0	35.0	40.0	29.0	41.0
37	35.56477410257264	38.0	35.0	40.0	28.0	41.0
38	35.37483344991839	38.0	34.0	40.0	27.0	41.0
39	35.283092113298466	38.0	34.0	40.0	27.0	41.0
40	35.03481451872578	38.0	34.0	40.0	26.0	41.0
41	34.823663713178554	38.0	34.0	40.0	25.0	40.0
42	34.836336092513015	38.0	34.0	40.0	26.0	40.0
43	34.71363101384586	37.0	34.0	40.0	25.0	40.0
44	34.49226652454393	37.0	34.0	40.0	24.0	40.0
45	34.30053337663635	37.0	33.0	40.0	24.0	40.0
46	34.208669903289916	37.0	33.0	39.0	24.0	40.0
47	34.01511788969943	36.0	33.0	39.0	24.0	40.0
48	33.90390129686997	36.0	33.0	39.0	23.0	40.0
49	33.74905274641085	36.0	33.0	39.0	24.0	40.0
50	33.85593417940775	36.0	33.0	39.0	24.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
10	1.0
11	3.0
12	2.0
13	7.0
14	7.0
15	27.0
16	92.0
17	242.0
18	504.0
19	1093.0
20	1889.0
21	3379.0
22	5394.0
23	8229.0
24	12148.0
25	17360.0
26	22458.0
27	24042.0
28	25776.0
29	28848.0
30	34972.0
31	46124.0
32	62366.0
33	102539.0
34	115058.0
35	150449.0
36	212425.0
37	369071.0
38	154553.0
39	41950.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	35.843312459056435	23.169337019641805	25.815817816417397	15.171532704884358
2	34.99925052463276	23.548863018109543	28.88887500971542	12.563011447542276
3	31.199410412711103	23.63789791590331	32.072826799018465	13.089864872367121
4	27.789019908286424	26.490900813874735	30.219748953510322	15.500330324328527
5	17.12419362002154	47.92707604676726	23.455525576540868	11.49320475667033
6	20.569767829186237	40.67312603399842	29.023364200615127	9.733741936200216
7	38.901865916080965	55.747990295681916	4.261114442112744	1.0890293461243796
8	86.18800173212084	3.1765264314979516	8.552138503047866	2.083333333333333
9	81.20183926806791	5.064371606542087	10.39876253289364	3.3350265924963636
10	43.06069084973851	26.20013212973141	16.933563172445954	13.80561384808412
11	35.95975872444844	24.206041881793855	23.623116596160465	16.211082797597236
12	32.33972330479775	22.136032554989285	28.97888144965191	16.545362690561053
13	23.649834005085328	29.647788214916226	29.330996080521416	17.37138169947703
14	19.560196751163076	32.5959328470071	30.84542209342349	16.99844830840634
15	18.22092590742036	23.88716787137892	39.52684509732076	18.36506112387995
16	20.194544374493407	20.209117506634243	38.49055661037274	21.105781508499604
17	20.474279115730102	20.50564604776656	30.743063189100962	28.27701164740237
18	23.08404949868425	23.678286310693625	32.422720762133174	20.814943428488945
19	27.33378301855368	25.175987919567415	26.82740137459334	20.662827687285567
20	27.47132562761622	25.048993482340137	26.851342948824712	20.628337941218923
21	24.481404683388295	26.093470681633967	29.237103472014038	20.188021162963704
22	23.570028757647425	23.434915003941686	27.896652898526586	25.0984033398843
23	21.76878962504025	27.712337474878694	27.68721617090259	22.831656729178466
24	21.66046267612671	25.550517415586864	33.578925307840066	19.210094600446354
25	22.774890909696545	24.651424558364702	31.106975117417807	21.466709414520945
26	20.105717664301658	28.04509065876109	27.950642883315012	23.898548793622243
27	20.590517193520093	27.10123746710636	30.52113520535625	21.787110134017297
28	19.811340395056792	26.54613992427523	33.55775956830219	20.08476011236579
29	20.147493976438717	25.43809611050043	32.585107091702476	21.829302821358382
30	22.340056404960972	26.124490634333746	31.537021307307107	19.99843165339818
31	27.5436361213817	24.774463431153748	26.74003197761567	20.941868469848885
32	26.68500105481718	25.510892375337264	27.128093667765896	20.676012902079655
33	25.9508621742558	25.08764698044702	26.74558364700265	22.215907198294527
34	21.471567125234557	26.35627283124035	29.362154824955866	22.810005218569223
35	22.35664201725459	25.25960993970887	29.16361324850382	23.220134794532715
36	27.779651466195887	26.29409413410613	25.386049209997445	20.54020518970054
37	22.10265314280004	29.710660870723828	26.750927128787627	21.43575885768851
38	22.516252512130396	30.82911406459922	24.461279881860477	22.193353541409902
39	21.99835115419207	28.012405205245216	25.446770593917588	24.542473046645128
40	23.50521301755438	25.723937688062797	27.364317200182093	23.406532094200728
41	20.958384686275163	24.673284256575954	28.080482551103113	26.28784850604577
42	22.090647657750687	27.336697644981843	25.57098919645137	25.001665500816095
43	22.189397976971676	26.73073293139247	26.699504791090682	24.380364300545175
44	21.675035808267545	27.197211951633854	28.20331323629015	22.924439003808445
45	20.95762133173445	30.14938154403029	25.991666944250134	22.90133017998512
46	23.197095366576733	28.289919278727115	27.60886823667877	20.90411711801739
47	22.576557520846517	27.242111077801095	28.001371262338587	22.1799601390138
48	22.182944161309308	27.059599946703973	28.52565704007195	22.23179885191477
49	22.296128820936456	25.987572588077235	29.304139879861875	22.412158711124437
50	21.302726980002888	28.262091535924856	28.753275484938325	21.681905999133942
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	901.0
1	1591.0
2	2281.0
3	11393.0
4	20505.0
5	14597.5
6	8690.0
7	8551.0
8	8412.0
9	8553.0
10	8694.0
11	8479.5
12	8265.0
13	8061.0
14	7857.0
15	7530.0
16	7203.0
17	6918.0
18	6633.0
19	6538.0
20	6443.0
21	6958.5
22	7474.0
23	7953.0
24	8432.0
25	9642.0
26	10852.0
27	13668.0
28	16484.0
29	19437.0
30	22390.0
31	25186.0
32	27982.0
33	32783.5
34	37585.0
35	39851.0
36	42117.0
37	46486.0
38	50855.0
39	61815.0
40	72775.0
41	84281.5
42	95788.0
43	99526.0
44	103264.0
45	106936.0
46	110608.0
47	113187.5
48	115767.0
49	112124.0
50	108481.0
51	101504.5
52	94528.0
53	87069.0
54	79610.0
55	76319.0
56	73028.0
57	70407.5
58	67787.0
59	62051.5
60	56316.0
61	50984.0
62	45652.0
63	39847.5
64	34043.0
65	29013.5
66	23984.0
67	20849.0
68	17714.0
69	15508.0
70	13302.0
71	11135.0
72	8968.0
73	6847.0
74	4726.0
75	3505.0
76	2284.0
77	1720.5
78	1157.0
79	915.5
80	674.0
81	475.5
82	277.0
83	200.5
84	124.0
85	95.0
86	66.0
87	42.0
88	18.0
89	14.0
90	10.0
91	5.0
92	0.0
93	0.5
94	1.0
95	1.0
96	1.0
97	0.5
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
50	1441008.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	28.204896913440514
#Duplication Level	Percentage of deduplicated	Percentage of total
1	70.17989548684056	19.794167176023674
2	7.598967424136662	4.286561856927342
3	3.8489783831243067	3.2568011555424596
4	2.6208064899522143	2.956783075167123
5	1.9485611174910373	2.7479482724186575
6	1.5677732672947018	2.6531330032616927
7	1.3070258575807603	2.5805170703386566
8	1.104216740869055	2.4915455477045563
9	0.9473971888926763	2.404911604392114
>10	8.544214949887195	47.769971323431555
>50	0.2876601959901359	5.106524176124764
>100	0.03919791673609427	2.0424841427876546
>500	0.004126096492437253	0.7699943265813212
>1k	8.841635340936973E-4	0.5911075954139884
>5k	2.947211780312324E-4	0.5475496738843962
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CGTTTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	6587	0.45711057815084993	No Hit
CGTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	2896	0.20097043180884488	No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	2781	0.19298990706505445	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.008327504080477	0.0
2	0.0	0.0	0.0	0.025051908108768308	0.0
3	0.0	0.0	0.0	0.03469793366865417	0.0
4	0.0	0.0	0.0	0.052185692237655866	0.0
5	0.0	0.0	0.0	0.09708481840489436	0.0
6	0.0	0.0	0.0	0.19354507400375293	0.0
7	0.0	0.0	0.0	0.26092846118827934	0.0
8	0.0	0.0	0.0	0.6646736173567391	0.0
9	0.0	0.0	0.0	0.8549570855956387	0.0
10	0.0	0.0	0.0	1.160229506012458	0.0
11	0.0	0.0	0.0	1.3523866626694647	0.0
12	0.0	0.0	0.0	1.5391309416741614	0.0
13	0.0	0.0	0.0	1.619838335387451	0.0
14	0.0	0.0	0.0	1.652246205433974	0.0
15	0.0	0.0	0.0	1.6957574142544662	0.0
16	0.0	0.0	0.0	1.7818082897527285	0.0
17	0.0	0.0	0.0	1.886318465962715	0.0
18	0.0	0.0	0.0	2.0455819815018375	0.0
19	0.0	0.0	0.0	2.111855034808967	0.0
20	0.0	0.0	0.0	2.189994781430776	0.0
21	0.0	0.0	0.0	2.2817361180506976	0.0
22	0.0	0.0	0.0	2.3766696645681358	0.0
23	0.0	0.0	0.0	2.4893685531239242	0.0
24	0.0	0.0	0.0	2.5779176798463297	0.0
25	0.0	0.0	0.0	2.647035963714289	0.0
26	0.0	0.0	0.0	2.7183055194697046	0.0
27	0.0	0.0	0.0	2.793530639663347	0.0
28	0.0	0.0	0.0	2.880830640773681	0.0
29	0.0	0.0	0.0	2.9723637897915904	0.0
30	0.0	0.0	0.0	3.089504013856967	0.0
31	0.0	0.0	0.0	3.189989229761389	0.0
32	0.0	0.0	0.0	3.2913765919411966	0.0
33	0.0	0.0	0.0	3.3913066409069206	0.0
34	0.0	0.0	0.0	3.4910285022706327	0.0
35	0.0	0.0	0.0	3.612401874243585	0.0
36	0.0	0.0	0.0	3.7121931314746344	0.0
37	0.0	0.0	0.0	3.8135804936544417	0.0
38	0.0	0.0	0.0	3.9442529118505933	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TATCACG	20	7.857146E-4	44.000004	19
CGTTTAG	65	0.0	44.000004	2
CGATACG	30	2.528166E-6	44.000004	10
CGTTTTA	3515	0.0	43.56188	1
CGGTCTA	370	0.0	42.81081	31
CGTTATT	1890	0.0	42.37037	1
AGGCGAT	1000	0.0	41.8	7
GCGATAT	140	0.0	40.857143	9
ATAACGC	115	0.0	40.173916	11
GTTTTAT	4170	0.0	39.621105	2
CGACGGT	395	0.0	39.544304	28
AGGTACC	495	0.0	39.11111	7
TCGAATA	45	2.3515895E-8	39.11111	1
CGTTTTT	1910	0.0	38.586384	1
CAAGGAC	1095	0.0	38.374428	5
TAACGGG	300	0.0	38.13333	3
TCTCACG	410	0.0	38.097565	23
ATAGGCG	185	0.0	38.054054	5
TCACGAC	405	0.0	38.024693	25
AGGCGCG	640	0.0	37.812504	7
>>END_MODULE