FastQCFastQC Report
Thu 26 May 2016
SRR1545723_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1545723_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences550681
Sequences flagged as poor quality0
Sequence length51
%GC46

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT16970.3081638916178332No Hit
CGTTTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT16300.29599713808902073No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATAC5790.10514254168929016No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGGGTAT207.0300803E-445.0000046
AGCGTAA207.0300803E-445.0000041
AAATCGT207.0300803E-445.00000412
TAACGCC406.8030204E-945.00000412
ATATACG207.0300803E-445.0000041
TTACGAG207.0300803E-445.0000041
ACGTATA207.0300803E-445.00000413
TAGCGAA302.163435E-644.9999961
CGTTAAG600.044.9999962
GAACGTT302.163435E-644.99999639
CGTTTTA8250.043.9090921
CGGTCTA1350.043.33333231
GGTATGC700.041.7857178
CGTTTTT12150.041.2962951
CGCAGTA1200.041.24999637
AGGTACC2400.040.3124967
ACGACGG1400.040.17857427
ATAACGC451.9252184E-840.00000411
GGTTAAC451.9252184E-840.0000048
CAAGGAC4450.039.943825