##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1545723_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	550681
Sequences flagged as poor quality	0
Sequence length	51
%GC	46
>>END_MODULE
>>Per base sequence quality	fail
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	30.945894265464034	31.0	31.0	33.0	30.0	33.0
2	31.109322820289787	31.0	31.0	33.0	30.0	34.0
3	31.050190582206394	31.0	31.0	34.0	28.0	34.0
4	35.04166659100277	35.0	35.0	37.0	32.0	37.0
5	32.75412988644969	35.0	35.0	37.0	28.0	37.0
6	33.944140073835854	35.0	35.0	37.0	28.0	37.0
7	15.321678067701628	0.0	0.0	35.0	0.0	37.0
8	24.89023409196976	17.0	17.0	35.0	17.0	37.0
9	32.579469783776815	32.0	32.0	37.0	27.0	39.0
10	35.344900223541394	37.0	34.0	37.0	32.0	39.0
11	36.33701544088138	37.0	35.0	39.0	32.0	39.0
12	36.34996122982271	37.0	35.0	39.0	32.0	39.0
13	36.20922457829487	38.0	35.0	39.0	32.0	39.0
14	37.258153086814325	39.0	36.0	40.0	32.0	41.0
15	37.50453166170614	39.0	36.0	41.0	32.0	41.0
16	37.48371743350506	39.0	36.0	41.0	32.0	41.0
17	37.427541534935834	39.0	36.0	41.0	32.0	41.0
18	37.34913498014277	39.0	36.0	40.0	32.0	41.0
19	37.30645328239035	39.0	36.0	40.0	32.0	41.0
20	37.4156326439445	39.0	36.0	40.0	32.0	41.0
21	37.32220468837676	39.0	35.0	40.0	32.0	41.0
22	37.37124578476468	39.0	35.0	41.0	32.0	41.0
23	37.33046355330945	39.0	35.0	41.0	32.0	41.0
24	37.151172820562174	39.0	35.0	40.0	32.0	41.0
25	36.893934964162554	39.0	35.0	40.0	31.0	41.0
26	36.78155774395703	39.0	35.0	40.0	31.0	41.0
27	36.70749853363381	39.0	35.0	40.0	31.0	41.0
28	36.58528621833693	38.0	35.0	40.0	31.0	41.0
29	36.526896696998804	38.0	35.0	40.0	30.0	41.0
30	36.23917839910947	38.0	35.0	40.0	30.0	41.0
31	36.12412812499433	38.0	35.0	40.0	30.0	41.0
32	36.05037035960928	38.0	35.0	40.0	30.0	41.0
33	35.878479555314236	38.0	35.0	40.0	29.0	41.0
34	35.678686934904235	38.0	35.0	40.0	27.0	41.0
35	35.49774188686372	38.0	34.0	40.0	27.0	41.0
36	35.291985741291235	38.0	34.0	40.0	25.0	41.0
37	35.168870907113195	38.0	34.0	40.0	25.0	41.0
38	35.00991136429258	38.0	34.0	40.0	25.0	41.0
39	34.89358448902359	38.0	34.0	40.0	24.0	41.0
40	34.74365921468146	38.0	34.0	40.0	24.0	41.0
41	34.58850586818866	38.0	33.0	40.0	23.0	41.0
42	34.53547335026994	38.0	33.0	40.0	23.0	41.0
43	34.4455229070914	38.0	33.0	40.0	23.0	41.0
44	34.221300535155564	37.0	33.0	40.0	23.0	41.0
45	34.15210621031051	37.0	33.0	40.0	23.0	41.0
46	34.09210958794656	37.0	33.0	40.0	23.0	41.0
47	33.98852693301566	37.0	33.0	40.0	23.0	41.0
48	33.91215059172189	37.0	33.0	40.0	23.0	41.0
49	33.787713758055936	36.0	33.0	40.0	22.0	41.0
50	33.68259663943372	36.0	33.0	40.0	22.0	41.0
51	33.508361465167674	36.0	32.0	40.0	21.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
12	1.0
13	4.0
14	16.0
15	30.0
16	75.0
17	184.0
18	403.0
19	713.0
20	1247.0
21	1997.0
22	2928.0
23	4370.0
24	6164.0
25	8275.0
26	9687.0
27	10651.0
28	11696.0
29	13321.0
30	15944.0
31	20599.0
32	26687.0
33	37951.0
34	47437.0
35	54817.0
36	69475.0
37	102221.0
38	85107.0
39	18681.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	35.46082032973718	23.998830538914547	25.877413602430444	14.662935528917831
2	34.5888091290602	23.417005489566556	29.380893838719697	12.61329154265355
3	31.771570110463227	23.407744229417755	31.676596795603988	13.144088864515027
4	28.326926115119278	27.035434307702644	28.881148977357128	15.75649059982095
5	21.065371785116973	36.53004189358267	28.28824673449783	14.116339586802523
6	20.661871391967402	41.32701146398732	28.508519451370212	9.50259769267507
7	37.91977569591106	56.617170376315876	4.612470740773697	0.8505831869993699
8	86.65034747884891	3.5143758364643047	7.854274979525351	1.9810017051614275
9	81.50163161612622	5.326314145576114	9.664397355274652	3.507656883023021
10	42.95045589007066	26.914856332432024	16.38534832325793	13.749339454239387
11	36.26182853594004	24.752261291019664	22.901098821277653	16.084811351762635
12	32.383902840301374	22.677557424352756	28.54810679867292	16.390432936672955
13	23.887513823792723	30.36694565456226	28.46457386399749	17.280966657647532
14	19.387812544830854	33.59858066648386	30.204964398626426	16.808642390058854
15	18.13372896468191	24.43992075266806	39.042748887286834	18.383601395363193
16	20.119633689922114	20.66205298530365	38.023283897574096	21.19502942720014
17	20.390207760936004	21.046486078146877	30.18208363825881	28.381222522658305
18	23.24122314007565	24.053490133126072	31.94027031984034	20.76501640695793
19	27.583664589844208	25.389653901260438	26.21517720785718	20.81150430103817
20	27.85659937422936	25.376942367722876	26.254946148496135	20.511512109551628
21	24.530172640784773	26.70802152244221	28.67758284741983	20.084222989353183
22	23.49581699749946	23.805978415816053	27.458728374503572	25.239476212180918
23	21.76777480973558	28.087404504604297	27.29965261194775	22.845168073712365
24	21.56457186647079	26.15743052692938	33.3419892823613	18.936008324238536
25	22.83536203355482	25.244197638923442	30.731766667090383	21.188673660431355
26	20.029018615132898	28.677219660747326	27.414238007122087	23.87952371699768
27	20.54292775672304	27.901634521619595	30.007390848785416	21.548046872871954
28	19.787499477919155	27.166181509803316	33.17219951296667	19.87411949931085
29	20.13107407010592	25.903018262841826	32.29256139216715	21.6733462748851
30	22.28549741138699	26.786651437038866	31.11819728663237	19.809653864941772
31	27.832265867171742	25.266715212618557	26.180311287297002	20.720707632912703
32	27.04070051445392	25.769002380688637	26.78138523028759	20.408911874569853
33	26.28854091570256	25.388927527915435	26.204826387690876	22.117705168691128
34	21.412033464020006	26.713469322529743	28.94615939173496	22.928337821715296
35	22.416789393496416	25.424883008493122	29.046580506681728	23.111747091328734
36	27.781056546348974	26.657901761636953	25.13506004383663	20.425981648177437
37	21.958810999471563	30.209504232032703	26.53677900635758	21.294905762138153
38	22.45637674079912	31.34809445032605	24.117592580822656	22.077936228052174
39	21.974609619725392	28.320388755014246	25.32264596018384	24.382355665076513
40	23.599869979171245	25.960220163760873	27.1433007494357	23.296609107632186
41	20.875788342071	24.94656616080816	27.909806221750888	26.26783927536995
42	21.927940132308905	27.541353342497743	25.264354499247293	25.266352025946055
43	22.051060414287036	26.886890958649378	26.578363880359046	24.483684746704533
44	21.462879598170264	27.242087524356208	28.47111122410252	22.823921653371006
45	20.917191622736212	30.741572707247933	25.689827686083234	22.65140798393262
46	23.205449252834217	28.783451762454128	27.186519963463418	20.824579021248233
47	22.479983874511742	27.80339252670784	27.470713534696127	22.24591006408429
48	21.830242917405904	27.62179919045691	28.551738665397934	21.996219226739257
49	22.266974891089397	26.57872706703155	29.08834697401944	22.06595106785961
50	21.270027475071775	28.93435582487865	28.424260143349777	21.371356556699794
51	21.104595945747175	29.240885376470228	27.07774555504911	22.57677312273349
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	374.0
1	642.0
2	910.0
3	3610.5
4	6311.0
5	4588.0
6	2865.0
7	2846.5
8	2828.0
9	2849.0
10	2870.0
11	2884.5
12	2899.0
13	2787.0
14	2675.0
15	2593.5
16	2512.0
17	2400.5
18	2289.0
19	2382.0
20	2475.0
21	2641.0
22	2807.0
23	2958.0
24	3109.0
25	3596.0
26	4852.5
27	5622.0
28	6871.0
29	8120.0
30	9145.5
31	10171.0
32	12128.5
33	14086.0
34	15123.5
35	16161.0
36	17364.5
37	18568.0
38	21911.0
39	25254.0
40	29934.0
41	34614.0
42	36532.5
43	38451.0
44	38999.0
45	39547.0
46	41019.0
47	42491.0
48	41289.5
49	40088.0
50	39174.5
51	38261.0
52	35786.0
53	33311.0
54	31071.5
55	28832.0
56	27690.5
57	26549.0
58	24743.5
59	22938.0
60	20694.0
61	18450.0
62	16484.5
63	14519.0
64	12756.0
65	10993.0
66	9337.5
67	7682.0
68	6815.0
69	5948.0
70	5325.0
71	4702.0
72	3919.0
73	3136.0
74	2374.0
75	1178.0
76	744.0
77	566.0
78	388.0
79	319.0
80	250.0
81	173.0
82	96.0
83	70.5
84	45.0
85	37.5
86	30.0
87	20.0
88	10.0
89	6.5
90	3.0
91	1.5
92	0.0
93	1.0
94	2.0
95	1.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	550681.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	39.87335812112977
#Duplication Level	Percentage of deduplicated	Percentage of total
1	67.81039135792926	27.038280189486763
2	9.919200196690122	7.910236434356122
3	5.363221948188055	6.415490082696169
4	3.6119911878388438	5.760888726522524
5	2.6050472873845454	5.193599170618081
6	2.025117083969962	4.84489312358114
7	1.6314159617793553	4.553502302198892
8	1.234685403021972	3.9384842593301226
9	1.0373320105229633	3.7225719671484296
>10	4.71200341607949	27.757396822349783
>50	0.028854776213267683	0.7496466501913862
>100	0.019386802748573455	1.4128232659482094
>500	4.508558778738013E-4	0.10408762832218006
>1k	9.017117557476025E-4	0.5980993772502471
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1697	0.3081638916178332	No Hit
CGTTTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1630	0.29599713808902073	No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATAC	579	0.10514254168929016	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.007990106795041049	0.0
2	0.0	0.0	0.0	0.026694220428887142	0.0
3	0.0	0.0	0.0	0.035410700568931926	0.0
4	0.0	0.0	0.0	0.05211728750401775	0.0
5	0.0	0.0	0.0	0.09806040157550379	0.0
6	0.0	0.0	0.0	0.1599837292370719	0.0
7	0.0	0.0	0.0	0.20374772327354676	0.0
8	0.0	0.0	0.0	0.5373346819665106	0.0
9	0.0	0.0	0.0	0.6987711578935899	0.0
10	0.0	0.0	0.0	0.9439221618323494	0.0
11	0.0	0.0	0.0	1.097186937628137	0.0
12	0.0	0.0	0.0	1.2580786335464633	0.0
13	0.0	0.0	0.0	1.329808001365582	0.0
14	0.0	0.0	0.0	1.3552310684407125	0.0
15	0.0	0.0	0.0	1.3926392957084046	0.0
16	0.0	0.0	0.0	1.4739931103488226	0.0
17	0.0	0.0	0.0	1.5555285183254914	0.0
18	0.0	0.0	0.0	1.6964449472562155	0.0
19	0.0	0.0	0.0	1.7512861348039972	0.0
20	0.0	0.0	0.0	1.822470722614363	0.0
21	0.0	0.0	0.0	1.9049140972722864	0.0
22	0.0	0.0	0.0	1.9849967585589479	0.0
23	0.0	0.0	0.0	2.0799700734181856	0.0
24	0.0	0.0	0.0	2.14498048779602	0.0
25	0.0	0.0	0.0	2.20345354206882	0.0
26	0.0	0.0	0.0	2.2653768697303884	0.0
27	0.0	0.0	0.0	2.334563930841994	0.0
28	0.0	0.0	0.0	2.4033878052810973	0.0
29	0.0	0.0	0.0	2.4834704665677587	0.0
30	0.0	0.0	0.0	2.5946055883533297	0.0
31	0.0	0.0	0.0	2.677412149683755	0.0
32	0.0	0.0	0.0	2.7605818976866825	0.0
33	0.0	0.0	0.0	2.8502890057946435	0.0
34	0.0	0.0	0.0	2.93727221385884	0.0
35	0.0	0.0	0.0	3.047862555635658	0.0
36	0.0	0.0	0.0	3.144107023848653	0.0
37	0.0	0.0	0.0	3.229637485222842	0.0
38	0.0	0.0	0.0	3.3536657338822295	0.0
39	0.0	0.0	0.0	3.5220027565868444	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGGGTAT	20	7.0300803E-4	45.000004	6
AGCGTAA	20	7.0300803E-4	45.000004	1
AAATCGT	20	7.0300803E-4	45.000004	12
TAACGCC	40	6.8030204E-9	45.000004	12
ATATACG	20	7.0300803E-4	45.000004	1
TTACGAG	20	7.0300803E-4	45.000004	1
ACGTATA	20	7.0300803E-4	45.000004	13
TAGCGAA	30	2.163435E-6	44.999996	1
CGTTAAG	60	0.0	44.999996	2
GAACGTT	30	2.163435E-6	44.999996	39
CGTTTTA	825	0.0	43.909092	1
CGGTCTA	135	0.0	43.333332	31
GGTATGC	70	0.0	41.785717	8
CGTTTTT	1215	0.0	41.296295	1
CGCAGTA	120	0.0	41.249996	37
AGGTACC	240	0.0	40.312496	7
ACGACGG	140	0.0	40.178574	27
ATAACGC	45	1.9252184E-8	40.000004	11
GGTTAAC	45	1.9252184E-8	40.000004	8
CAAGGAC	445	0.0	39.94382	5
>>END_MODULE