FastQCFastQC Report
Thu 26 May 2016
SRR1545722_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1545722_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences779060
Sequences flagged as poor quality0
Sequence length50
%GC44

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT31520.4045901471003517No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATA18920.24285677611480505No Hit
CGTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT14140.18150078299489128No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT12760.16378712807742665No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AACCGTA207.854919E-444.030
TACCCGC254.441516E-544.020
AAATCGT302.5266636E-644.012
ACACGCA207.854919E-444.044
CGTATGA207.854919E-444.015
CGCGGAC254.441516E-544.030
TTGTCTA207.854919E-444.01
GCTATGC254.441516E-544.043
TCGGGTA408.307325E-944.027
CGTCTCA207.854919E-444.020
CGATTTC207.854919E-444.023
CGCTGAT207.854919E-444.043
CCGCTAT207.854919E-444.024
CCGTCAT207.854919E-444.031
ATGTACG302.5266636E-644.01
ACGATTA351.445278E-744.01
AACGGCT302.5266636E-644.039
CCGTAGA207.854919E-444.03
CTAGTAA551.8189894E-1244.01
TGCGATG207.854919E-444.01
Produced by FastQC (version 0.11.5)