##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1545722_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	779060
Sequences flagged as poor quality	0
Sequence length	50
%GC	44
>>END_MODULE
>>Per base sequence quality	fail
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.52540240803019	31.0	31.0	33.0	30.0	34.0
2	31.708632197776808	31.0	31.0	34.0	30.0	34.0
3	31.73991605267887	31.0	31.0	34.0	30.0	34.0
4	35.63589068877879	37.0	35.0	37.0	33.0	37.0
5	27.099282468616025	35.0	25.0	37.0	0.0	37.0
6	31.324722100993505	35.0	26.0	37.0	17.0	37.0
7	15.003552999768953	0.0	0.0	32.0	0.0	37.0
8	25.10022334608374	17.0	17.0	35.0	17.0	37.0
9	33.20318717428696	32.0	32.0	37.0	28.0	39.0
10	36.09932611095423	37.0	35.0	39.0	32.0	39.0
11	37.074959566657256	39.0	37.0	39.0	34.0	39.0
12	36.85636793058301	39.0	35.0	39.0	33.0	39.0
13	36.22504685133366	39.0	35.0	39.0	32.0	39.0
14	37.524252304058734	40.0	35.0	41.0	33.0	41.0
15	37.85870921366775	40.0	35.0	41.0	33.0	41.0
16	37.97403665956409	40.0	35.0	41.0	33.0	41.0
17	38.01747490565553	40.0	35.0	41.0	34.0	41.0
18	37.98334017919031	40.0	36.0	41.0	34.0	41.0
19	37.921891767001256	40.0	36.0	41.0	34.0	41.0
20	37.90529612610069	40.0	35.0	41.0	34.0	41.0
21	37.74106487305214	40.0	35.0	41.0	33.0	41.0
22	37.64668318229661	39.0	35.0	41.0	33.0	41.0
23	37.54409031396811	39.0	35.0	41.0	33.0	41.0
24	37.383355582368495	39.0	35.0	41.0	33.0	41.0
25	37.195353374579625	39.0	35.0	41.0	33.0	41.0
26	36.93644391959541	38.0	35.0	40.0	33.0	41.0
27	36.6890226683439	38.0	35.0	40.0	32.0	41.0
28	36.745583138654276	38.0	35.0	40.0	32.0	41.0
29	36.73380484173234	38.0	35.0	40.0	32.0	41.0
30	36.5521397581701	38.0	35.0	40.0	32.0	41.0
31	36.307371704361664	38.0	35.0	40.0	31.0	41.0
32	36.00126434420969	38.0	35.0	40.0	30.0	41.0
33	35.77290067517264	38.0	35.0	40.0	30.0	41.0
34	35.53095525376736	38.0	35.0	40.0	29.0	41.0
35	35.38289733781737	38.0	34.0	40.0	29.0	41.0
36	35.17888352630093	38.0	34.0	40.0	27.0	41.0
37	34.975257361435574	38.0	34.0	40.0	26.0	41.0
38	34.82827381716427	37.0	34.0	40.0	26.0	41.0
39	34.78300644366288	37.0	34.0	40.0	26.0	41.0
40	34.565512284034604	37.0	34.0	40.0	24.0	40.0
41	34.41093625651426	37.0	34.0	40.0	24.0	40.0
42	34.39884091084127	37.0	34.0	40.0	24.0	40.0
43	34.28569173106051	37.0	33.0	40.0	23.0	41.0
44	34.079619028059454	36.0	33.0	40.0	23.0	40.0
45	33.81569583857469	36.0	33.0	39.0	23.0	40.0
46	33.81585757194568	36.0	33.0	39.0	23.0	40.0
47	33.683430031063075	36.0	33.0	39.0	23.0	40.0
48	33.63694195569019	36.0	33.0	39.0	23.0	40.0
49	33.550012835981825	36.0	33.0	39.0	23.0	40.0
50	33.57750622545119	35.0	33.0	39.0	23.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
10	1.0
11	3.0
12	0.0
13	1.0
14	6.0
15	17.0
16	54.0
17	128.0
18	304.0
19	647.0
20	1247.0
21	2052.0
22	3271.0
23	5044.0
24	7678.0
25	11185.0
26	13884.0
27	14992.0
28	14898.0
29	16715.0
30	20148.0
31	26701.0
32	37556.0
33	73006.0
34	69930.0
35	81865.0
36	96875.0
37	181242.0
38	81707.0
39	17903.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	32.348214514928245	23.079993838728726	27.946499627756527	16.625292018586503
2	33.205273021333404	23.650809950453112	30.649243960670553	12.494673067542935
3	29.788206299899876	23.061638384720048	34.14165789541242	13.008497419967654
4	26.133545554899495	26.438400123225424	32.658203475983875	14.769850845891202
5	17.081739532256822	48.29936076810515	23.914589376941443	10.704310322696584
6	19.73031602187251	39.744949041152154	29.934408132878083	10.590326804097245
7	38.43311169871384	55.85667342695043	4.619926578183965	1.0902882961517726
8	86.01263060611505	3.1340333222088157	9.031910250815086	1.8214258208610377
9	81.83015428850152	4.699124586039586	10.676712961774445	2.79400816368444
10	55.0453110158396	20.107693887505455	14.59451133417195	10.252483762482992
11	51.19990758093087	17.558339537391216	19.280286499114318	11.961466382563604
12	43.76607706723487	20.364670243626932	23.530151721305163	12.33910096783303
13	21.305419351526197	41.95954098528997	24.364490539881395	12.370549123302442
14	14.473468025569275	44.726721946961725	28.44068492799014	12.359125099478858
15	12.633815110517805	21.49680384052576	53.29846224937745	12.570918799578982
16	14.223166379996405	15.026057043103227	51.58023772238338	19.170538854516984
17	15.256719636484995	15.441172695299462	30.689548943598695	38.61255872461685
18	20.816368444022284	21.072317921597822	37.50789412882191	20.60341950555798
19	29.915410879778193	21.78253279593356	27.312658845275074	20.98939747901317
20	32.14707467974225	21.755577234102635	25.634996020845634	20.462352065309474
21	21.836957358868382	26.449053988139553	30.068030703668526	21.645957949323545
22	22.77347059276564	22.798885836777654	26.57497496983544	27.85266860062126
23	19.444073627191745	29.6133802274536	25.59571791646343	25.346828228891226
24	19.80630503427207	22.6869560752702	40.719970220522164	16.786768669935565
25	17.315354401458166	22.245783379970735	39.63597155546428	20.802890663106822
26	16.71411701281031	33.38420660796345	28.68071778810361	21.220958591122635
27	17.284162965625242	32.71018920237209	31.356121479731986	18.649526352270684
28	14.392344620440017	27.482350524991656	41.789977665391625	16.3353271891767
29	15.19125612918132	23.4816317100095	40.36390008471748	20.9632120760917
30	18.59266295278926	28.20938053551716	33.78622955869894	19.411726952994634
31	30.14235103843093	24.605550278540804	25.76784843272662	19.484250250301645
32	30.426796395656304	25.512797473878777	27.63933458270223	16.42107154776269
33	27.82057864606064	26.23058557749082	25.929453443893923	20.019382332554617
34	19.600415885811106	27.2943033912664	28.91651477421508	24.188765948707417
35	20.335404205067643	24.733268297692092	31.704618386260364	23.226709110979897
36	32.32780530382769	23.00182270941904	25.863348137499038	18.80702384925423
37	20.40099607218956	32.173388442481965	29.031781890996843	18.39383359433163
38	21.521063846173593	33.009139219058866	23.164968038405256	22.304828896362284
39	20.967191230457217	30.531152927887454	27.340898005288423	21.1607578363669
40	23.381125972325624	24.985366980720354	26.49205452725079	25.141452519703233
41	17.794778322593896	23.7776294508767	27.30559905527174	31.121993171257667
42	22.55808281775473	25.679793597412264	24.319179524041797	27.44294406079121
43	23.011578055605472	25.627037712114596	25.764896156907042	25.596488075372886
44	19.77139116371011	29.96701152671168	28.741175262495826	21.520422047082384
45	17.01486406695248	37.50430005391112	23.420018997253102	22.060816881883294
46	22.849331245346956	31.54108797781942	27.162477857931357	18.447102918902267
47	23.088208867096245	27.05837804533669	26.59037814802454	23.263034939542525
48	23.782635483788155	22.901830411008138	30.857186866223397	22.45834723898031
49	21.395656303750673	22.75344646111981	33.18152645495854	22.669370780170976
50	20.059815675301003	32.27813005416784	26.706928862988732	20.955125407542425
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	498.0
1	743.5
2	989.0
3	5225.0
4	9461.0
5	7108.0
6	4755.0
7	5053.5
8	5352.0
9	5771.0
10	6190.0
11	6235.5
12	6281.0
13	5904.0
14	5527.0
15	5134.5
16	4742.0
17	4328.5
18	3915.0
19	3734.5
20	3554.0
21	3648.5
22	3743.0
23	3795.0
24	3847.0
25	4071.0
26	4295.0
27	5251.0
28	6207.0
29	7200.0
30	8193.0
31	9734.0
32	11275.0
33	12435.0
34	13595.0
35	14424.5
36	15254.0
37	20407.0
38	25560.0
39	38126.5
40	50693.0
41	64515.0
42	78337.0
43	81200.0
44	84063.0
45	81423.0
46	78783.0
47	72975.0
48	67167.0
49	62461.5
50	57756.0
51	53422.0
52	49088.0
53	43543.0
54	37998.0
55	33615.0
56	29232.0
57	27622.5
58	26013.0
59	24473.0
60	22933.0
61	20246.5
62	17560.0
63	14894.0
64	12228.0
65	10264.5
66	8301.0
67	7061.0
68	5821.0
69	5025.5
70	4230.0
71	3422.0
72	2614.0
73	2055.0
74	1496.0
75	1150.0
76	804.0
77	590.5
78	377.0
79	273.5
80	170.0
81	132.0
82	94.0
83	70.5
84	47.0
85	28.5
86	10.0
87	5.5
88	1.0
89	1.0
90	1.0
91	1.5
92	2.0
93	2.5
94	3.0
95	4.0
96	5.0
97	2.5
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
50	779060.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	22.454484184143862
#Duplication Level	Percentage of deduplicated	Percentage of total
1	73.60434410685001	16.527475806315458
2	5.997946430636169	2.6936158652812394
3	2.6310384866337655	1.77235836257975
4	1.6795074558256136	1.5084989441595174
5	1.32487015498171	1.4874637970540516
6	1.0974510365058694	1.4785618165255998
7	0.898262573564728	1.4119015925922282
8	0.8316101955057289	1.4938702385884939
9	0.771040325687385	1.5581981518636066
>10	9.933412369024332	49.89076407148797
>50	1.1390153692864622	16.31740960561272
>100	0.08912483327750091	2.8280361346331495
>500	0.0	0.0
>1k	0.0023766622207333577	1.0318456133062592
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CGTTTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	3152	0.4045901471003517	No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATA	1892	0.24285677611480505	No Hit
CGTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1414	0.18150078299489128	No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1276	0.16378712807742665	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.00924190691345981	0.0
2	0.0	0.0	0.0	0.032346674197109335	0.0
3	0.0	0.0	0.0	0.04415577747541909	0.0
4	0.0	0.0	0.0	0.06713218494082612	0.0
5	0.0	0.0	0.0	0.12065822914794752	0.0
6	0.0	0.0	0.0	0.20242343336841834	0.0
7	0.0	0.0	0.0	0.26493466485251455	0.0
8	0.0	0.0	0.0	0.6158704079275024	0.0
9	0.0	0.0	0.0	0.7768336200035941	0.0
10	0.0	0.0	0.0	1.0560162246810259	0.0
11	0.0	0.0	0.0	1.2221138294868175	0.0
12	0.0	0.0	0.0	1.415552075578261	0.0
13	0.0	0.0	0.0	1.4833260596102995	0.0
14	0.0	0.0	0.0	1.5037352707108567	0.0
15	0.0	0.0	0.0	1.5428850152748184	0.0
16	0.0	0.0	0.0	1.6335070469540216	0.0
17	0.0	0.0	0.0	1.7395322568223244	0.0
18	0.0	0.0	0.0	1.9256539932739456	0.0
19	0.0	0.0	0.0	1.9926578183965291	0.0
20	0.0	0.0	0.0	2.054527250789413	0.0
21	0.0	0.0	0.0	2.1419402870125537	0.0
22	0.0	0.0	0.0	2.2280697250532695	0.0
23	0.0	0.0	0.0	2.335121813467512	0.0
24	0.0	0.0	0.0	2.4129078633224657	0.0
25	0.0	0.0	0.0	2.4675891458937693	0.0
26	0.0	0.0	0.0	2.5281749801042284	0.0
27	0.0	0.0	0.0	2.591584730316022	0.0
28	0.0	0.0	0.0	2.650245167252843	0.0
29	0.0	0.0	0.0	2.7199445485585194	0.0
30	0.0	0.0	0.0	2.8146740944214823	0.0
31	0.0	0.0	0.0	2.902343850281108	0.0
32	0.0	0.0	0.0	2.984879213411034	0.0
33	0.0	0.0	0.0	3.0586861089004698	0.0
34	0.0	0.0	0.0	3.1422483505763354	0.0
35	0.0	0.0	0.0	3.2590557851770083	0.0
36	0.0	0.0	0.0	3.352630092675789	0.0
37	0.0	0.0	0.0	3.4467178394475395	0.0
38	0.0	0.0	0.0	3.5340025158524377	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
AACCGTA	20	7.854919E-4	44.0	30
TACCCGC	25	4.441516E-5	44.0	20
AAATCGT	30	2.5266636E-6	44.0	12
ACACGCA	20	7.854919E-4	44.0	44
CGTATGA	20	7.854919E-4	44.0	15
CGCGGAC	25	4.441516E-5	44.0	30
TTGTCTA	20	7.854919E-4	44.0	1
GCTATGC	25	4.441516E-5	44.0	43
TCGGGTA	40	8.307325E-9	44.0	27
CGTCTCA	20	7.854919E-4	44.0	20
CGATTTC	20	7.854919E-4	44.0	23
CGCTGAT	20	7.854919E-4	44.0	43
CCGCTAT	20	7.854919E-4	44.0	24
CCGTCAT	20	7.854919E-4	44.0	31
ATGTACG	30	2.5266636E-6	44.0	1
ACGATTA	35	1.445278E-7	44.0	1
AACGGCT	30	2.5266636E-6	44.0	39
CCGTAGA	20	7.854919E-4	44.0	3
CTAGTAA	55	1.8189894E-12	44.0	1
TGCGATG	20	7.854919E-4	44.0	1
>>END_MODULE