FastQCFastQC Report
Thu 26 May 2016
SRR1545721_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1545721_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences299798
Sequences flagged as poor quality0
Sequence length51
%GC44

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT8710.29052895616381696No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT7370.2458321936770759No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATAC7140.2381603613099487No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGGTAC650.045.0000046
TAGGCGA650.045.0000046
CGGGTAA207.0249353E-445.028
CATGCCG207.0249353E-445.012
AACCCGG253.883884E-545.038
CACGCTC207.0249353E-445.025
GTTAGGC406.7866495E-945.04
TGAGTTA253.883884E-545.01
CACTGTA207.0249353E-445.043
AGGCGAT2150.045.07
GCGTTCG253.883884E-545.044
CGTTCGG253.883884E-545.045
CAGGGTT207.0249353E-445.045
AACATAG207.0249353E-445.02
CAAGGCA207.0249353E-445.05
CGAATAC207.0249353E-445.040
ACGCTCA207.0249353E-445.026
GCGATAT253.883884E-545.09
GTATCAC207.0249353E-445.09
CGATGTT207.0249353E-445.010