##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1545721_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	299798
Sequences flagged as poor quality	0
Sequence length	51
%GC	44
>>END_MODULE
>>Per base sequence quality	fail
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	30.98304858604794	31.0	31.0	33.0	30.0	33.0
2	31.14042455253204	31.0	31.0	33.0	30.0	34.0
3	31.060177185971888	31.0	31.0	34.0	28.0	34.0
4	35.022658590117345	35.0	35.0	37.0	32.0	37.0
5	32.73347387240742	35.0	35.0	37.0	28.0	37.0
6	33.93663733580611	35.0	35.0	37.0	28.0	37.0
7	15.374405432991548	0.0	0.0	35.0	0.0	37.0
8	24.93621371723627	17.0	17.0	35.0	17.0	37.0
9	32.681922494479615	32.0	32.0	37.0	27.0	39.0
10	35.52733840786129	37.0	35.0	37.0	32.0	39.0
11	36.47776836403178	37.0	35.0	39.0	32.0	39.0
12	36.1469122542512	37.0	35.0	39.0	32.0	39.0
13	35.607722533172335	37.0	35.0	39.0	30.0	39.0
14	36.633002888611664	39.0	35.0	40.0	31.0	41.0
15	37.0942834842127	39.0	35.0	40.0	32.0	41.0
16	37.235685361476726	39.0	35.0	41.0	32.0	41.0
17	37.21136898845222	39.0	35.0	41.0	32.0	41.0
18	37.10916350342564	39.0	35.0	40.0	32.0	41.0
19	37.0239494593026	39.0	35.0	40.0	32.0	41.0
20	37.06825929459169	39.0	35.0	40.0	32.0	41.0
21	36.896400242830175	39.0	35.0	40.0	31.0	41.0
22	36.95171081861787	39.0	35.0	40.0	32.0	41.0
23	36.93462598149421	38.0	35.0	40.0	32.0	41.0
24	36.751976330729356	38.0	35.0	40.0	32.0	41.0
25	36.49498662432705	38.0	35.0	40.0	31.0	41.0
26	36.315338994923245	38.0	35.0	40.0	31.0	41.0
27	36.18235611978732	38.0	34.0	40.0	31.0	41.0
28	36.12966063816303	38.0	35.0	40.0	30.0	41.0
29	36.179304064736925	38.0	35.0	40.0	30.0	41.0
30	35.94743794154731	38.0	34.0	40.0	30.0	41.0
31	35.771706282230035	38.0	34.0	40.0	30.0	41.0
32	35.57051414619177	38.0	34.0	40.0	29.0	41.0
33	35.356646808851295	38.0	34.0	40.0	27.0	41.0
34	35.17148546688103	38.0	34.0	40.0	26.0	41.0
35	34.986717723267	38.0	34.0	40.0	25.0	41.0
36	34.76712653186479	38.0	34.0	40.0	24.0	41.0
37	34.56743207092776	37.0	33.0	40.0	23.0	41.0
38	34.51511017418395	37.0	33.0	40.0	23.0	41.0
39	34.45001967991781	37.0	33.0	40.0	23.0	41.0
40	34.27688643686749	37.0	33.0	40.0	23.0	41.0
41	34.192629704000694	37.0	33.0	40.0	23.0	41.0
42	34.120841366520125	37.0	33.0	40.0	23.0	41.0
43	34.02736175691632	37.0	33.0	40.0	23.0	41.0
44	33.791065984429515	36.0	33.0	40.0	22.0	41.0
45	33.698857230535225	36.0	33.0	40.0	22.0	41.0
46	33.697616395039326	36.0	33.0	40.0	22.0	41.0
47	33.66149207132803	36.0	33.0	40.0	22.0	41.0
48	33.661081795075354	36.0	33.0	40.0	22.0	41.0
49	33.58224871413418	36.0	33.0	40.0	21.0	41.0
50	33.415843334511905	35.0	32.0	40.0	20.0	41.0
51	33.19771646241803	35.0	32.0	39.0	20.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
12	1.0
13	5.0
14	8.0
15	17.0
16	58.0
17	121.0
18	260.0
19	455.0
20	739.0
21	1257.0
22	1845.0
23	2777.0
24	3838.0
25	4938.0
26	6030.0
27	6266.0
28	6855.0
29	7676.0
30	9253.0
31	11665.0
32	15679.0
33	24378.0
34	30321.0
35	30191.0
36	32840.0
37	49910.0
38	44437.0
39	7978.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	32.01855916316987	23.710298267500114	28.12727236339135	16.143870205938665
2	32.57660157839612	23.629910806609782	31.20868051154444	12.584807103449656
3	30.356773560864315	23.054189821146238	33.53524706635802	13.05378955163143
4	26.429128946824193	27.04220842033636	31.465853674807704	15.062808958031743
5	21.004476347407255	36.950213143516635	28.911467054483353	13.133843454592759
6	19.841026291035966	40.2954656135131	29.474846396573696	10.388661698877243
7	37.39084316773294	56.63380009206199	5.143796823194284	0.8315599170107872
8	86.4405366279962	3.517034803434312	8.286913188213397	1.7555153803561065
9	82.0689264104497	4.878284711705882	9.959039086318121	3.093749791526294
10	55.5370616214918	20.364045123716636	13.924709304264871	10.174183950526688
11	52.04337587308788	17.620864715575156	18.63588149353898	11.699877917797984
12	44.24245658743554	20.65991100674454	22.752319895396234	12.345312510423685
13	21.505813914702564	42.69341356513386	23.568536147672766	12.23223637249081
14	14.29729351096405	45.72645581358115	27.887444212436375	12.088806463018432
15	12.606154810906009	21.709284251395943	53.00468982448182	12.679871113216231
16	14.173877077232003	15.070147232469862	51.34623980146632	19.409735888831815
17	15.112842647382571	15.842667396046672	30.17064823647923	38.87384172009153
18	20.90641031627963	21.085197366226595	37.416193570337356	20.592198747156417
19	30.581925162943048	21.755315245598702	26.787703720505142	20.87505587095311
20	32.87046611384999	21.854381950513346	25.057538742753454	20.217613192883206
21	21.867390709744562	27.335072282003214	29.34909505733861	21.448441950913615
22	22.525166945743464	23.212629837423865	26.0071781666322	28.255025050200466
23	19.44075677622933	30.03055390629691	25.114243590684392	25.41444572678937
24	19.12054116438402	23.274004496360885	41.04597095377554	16.559483385479556
25	17.07182836443205	22.82937177699651	39.76110581124624	20.3376940473252
26	16.48309861973729	34.18134877484172	28.32507221529163	21.010480390129356
27	17.24661271923095	33.88648356560084	30.621618556494706	18.245285158673507
28	14.10249568042482	28.306726529196325	41.468922407754555	16.1218553826243
29	14.984422844715443	23.999826549876918	40.333491217419734	20.682259387987912
30	18.469769644894228	29.56023722639911	32.981540904208835	18.988452224497827
31	30.75937798117399	24.878084576948478	25.30203670471451	19.06050073716302
32	31.57892981274058	25.252003015363677	27.170961780932494	15.99810539096325
33	28.715001434299094	26.20931427160955	25.241662719564506	19.834021574526847
34	19.69059166505447	27.369095190761776	28.213330309074774	24.726982835108974
35	20.482124630584593	24.55119780652306	31.798744487955222	23.167933074937125
36	32.93384212036104	23.365065810979395	25.36974896430263	18.331343104356932
37	20.19359702199481	33.19168239948232	28.558562765595504	18.05615781292737
38	21.167586174690957	33.95486294104697	22.69227946817524	22.18527141608683
39	20.93076004509703	31.24904102095411	26.967157886310115	20.853041047638744
40	23.899425613246255	24.986824461804282	26.023856063082473	25.08989386186699
41	17.553486013916036	23.80469516140868	26.986837804121443	31.65498102055384
42	22.135904842594012	25.319715274951797	24.18461764254598	28.359762239908203
43	22.673266666221924	25.411443705428322	25.660277920466445	26.25501170788331
44	19.43441917557822	29.782053249187783	29.30106271556181	21.482464859672177
45	16.7249281182663	38.56496707783241	23.130574586888507	21.57953021701279
46	22.71062515427054	32.24237653353258	26.75201302210155	18.29498529009533
47	22.909759237886842	27.229000860579454	26.16628529876784	23.694954602765865
48	23.31536567955757	23.1722693280142	31.36478562231903	22.14757937010921
49	21.384398828544555	23.273337380502873	33.52557388641685	21.81668990453572
50	20.24229647962962	33.166665554806904	26.219321009479717	20.371716956083763
51	19.658570103869938	34.71170588196052	23.524172943115033	22.10555107105451
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	192.0
1	277.0
2	362.0
3	1690.0
4	3018.0
5	2330.0
6	1642.0
7	1754.5
8	1867.0
9	1949.5
10	2032.0
11	2014.5
12	1997.0
13	1952.5
14	1908.0
15	1769.5
16	1631.0
17	1542.0
18	1453.0
19	1372.5
20	1292.0
21	1369.5
22	1447.0
23	1476.0
24	1505.0
25	1446.0
26	1705.0
27	2023.0
28	2696.5
29	3370.0
30	3873.0
31	4376.0
32	4567.5
33	4759.0
34	5415.5
35	6072.0
36	7350.5
37	8629.0
38	12532.5
39	16436.0
40	21576.5
41	26717.0
42	29275.5
43	31834.0
44	31025.0
45	30216.0
46	28886.5
47	27557.0
48	24962.5
49	22368.0
50	21264.5
51	20161.0
52	18343.5
53	16526.0
54	14770.0
55	13014.0
56	11802.0
57	10590.0
58	9951.0
59	9312.0
60	8350.5
61	7389.0
62	6390.5
63	5392.0
64	4585.5
65	3779.0
66	3113.0
67	2447.0
68	2116.5
69	1786.0
70	1581.5
71	1377.0
72	1109.0
73	841.0
74	697.0
75	404.5
76	256.0
77	184.5
78	113.0
79	102.5
80	92.0
81	70.0
82	48.0
83	34.0
84	20.0
85	13.5
86	7.0
87	4.0
88	1.0
89	1.5
90	2.0
91	1.5
92	1.0
93	1.0
94	1.0
95	0.5
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	299798.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	30.902807890646365
#Duplication Level	Percentage of deduplicated	Percentage of total
1	69.5248580618699	21.485133323104225
2	7.164907281480043	4.428315065477421
3	3.8868380718001854	3.6034263070467447
4	2.6757766120501696	3.307560424018839
5	2.1058653368736913	3.253857597448949
6	1.7518295447186063	3.248187112655855
7	1.4215400556958746	3.0750705475019844
8	1.2229346113161927	3.0233690685061276
9	1.0826155473522872	3.011027425132923
>10	9.053817757917233	48.542018292316826
>50	0.09606458994452001	1.837570630891467
>100	0.009714396735962697	0.4099426947477968
>500	0.0032381322453208993	0.7745215111508416
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CGTTTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	871	0.29052895616381696	No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	737	0.2458321936770759	No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATAC	714	0.2381603613099487	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.006004042722099547	0.0
2	0.0	0.0	0.0	0.03368935082955857	0.0
3	0.0	0.0	0.0	0.04102762526768024	0.0
4	0.0	0.0	0.0	0.06837937544613373	0.0
5	0.0	0.0	0.0	0.12741912888011261	0.0
6	0.0	0.0	0.0	0.1797877237339809	0.0
7	0.0	0.0	0.0	0.21848044349862242	0.0
8	0.0	0.0	0.0	0.5110107472364726	0.0
9	0.0	0.0	0.0	0.6511050774187953	0.0
10	0.0	0.0	0.0	0.8962701552378601	0.0
11	0.0	0.0	0.0	1.036364485420183	0.0
12	0.0	0.0	0.0	1.1958051754848265	0.0
13	0.0	0.0	0.0	1.2571798344218441	0.0
14	0.0	0.0	0.0	1.2761926363751592	0.0
15	0.0	0.0	0.0	1.3182209354298562	0.0
16	0.0	0.0	0.0	1.3932714694561004	0.0
17	0.0	0.0	0.0	1.4843327840746103	0.0
18	0.0	0.0	0.0	1.6437734741392538	0.0
19	0.0	0.0	0.0	1.7038139013602491	0.0
20	0.0	0.0	0.0	1.7691912554453333	0.0
21	0.0	0.0	0.0	1.8472438108326272	0.0
22	0.0	0.0	0.0	1.9189587655688163	0.0
23	0.0	0.0	0.0	2.0160241229094256	0.0
24	0.0	0.0	0.0	2.080400803207493	0.0
25	0.0	0.0	0.0	2.1297673767003116	0.0
26	0.0	0.0	0.0	2.191475593566335	0.0
27	0.0	0.0	0.0	2.2391743774141255	0.0
28	0.0	0.0	0.0	2.291542972267994	0.0
29	0.0	0.0	0.0	2.346913588482912	0.0
30	0.0	0.0	0.0	2.4309701865923055	0.0
31	0.0	0.0	0.0	2.5153603426307045	0.0
32	0.0	0.0	0.0	2.583406160147833	0.0
33	0.0	0.0	0.0	2.6494506300909277	0.0
34	0.0	0.0	0.0	2.7258353958331942	0.0
35	0.0	0.0	0.0	2.8335746069019807	0.0
36	0.0	0.0	0.0	2.9096258147152416	0.0
37	0.0	0.0	0.0	2.986010580457508	0.0
38	0.0	0.0	0.0	3.063396019986791	0.0
39	0.0	0.0	0.0	3.1357780905809913	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
AAGGTAC	65	0.0	45.000004	6
TAGGCGA	65	0.0	45.000004	6
CGGGTAA	20	7.0249353E-4	45.0	28
CATGCCG	20	7.0249353E-4	45.0	12
AACCCGG	25	3.883884E-5	45.0	38
CACGCTC	20	7.0249353E-4	45.0	25
GTTAGGC	40	6.7866495E-9	45.0	4
TGAGTTA	25	3.883884E-5	45.0	1
CACTGTA	20	7.0249353E-4	45.0	43
AGGCGAT	215	0.0	45.0	7
GCGTTCG	25	3.883884E-5	45.0	44
CGTTCGG	25	3.883884E-5	45.0	45
CAGGGTT	20	7.0249353E-4	45.0	45
AACATAG	20	7.0249353E-4	45.0	2
CAAGGCA	20	7.0249353E-4	45.0	5
CGAATAC	20	7.0249353E-4	45.0	40
ACGCTCA	20	7.0249353E-4	45.0	26
GCGATAT	25	3.883884E-5	45.0	9
GTATCAC	20	7.0249353E-4	45.0	9
CGATGTT	20	7.0249353E-4	45.0	10
>>END_MODULE