FastQCFastQC Report
Thu 26 May 2016
SRR1545720_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1545720_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences993183
Sequences flagged as poor quality0
Sequence length50
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT34150.3438439844419407No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATA15940.16049408819925431No Hit
CGTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT14900.15002270477847487No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT13860.1395513213576954No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AACGTAT207.855964E-444.043
CGTATGG750.044.02
CAACGGC302.5273675E-644.036
CGCATAT254.4424025E-544.017
TACGGGT502.7284841E-1144.04
TACGATT207.855964E-444.035
TCCGTGA207.855964E-444.033
CGTTTTA23050.043.4273341
TTTACGG1450.042.4827582
TAGGCGA2650.042.3396226
TTACGGG2500.042.243
CGGTCTA2200.042.031
ACGGTCT2200.042.030
CGCATCG1050.041.90476221
AGGCGAT8850.041.7627147
CGTTATT11650.041.3562241
ACGGGTA1400.040.8571435
AATAGGC1950.040.6153874
TATACGG603.6379788E-1240.3333322
CGACGGT2300.040.1739128
Produced by FastQC (version 0.11.5)