##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1545720_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	993183
Sequences flagged as poor quality	0
Sequence length	50
%GC	45
>>END_MODULE
>>Per base sequence quality	fail
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.514481218466283	31.0	31.0	33.0	30.0	34.0
2	31.711005927407136	31.0	31.0	34.0	30.0	34.0
3	31.771000913225457	31.0	31.0	34.0	30.0	34.0
4	35.675157549011615	37.0	35.0	37.0	35.0	37.0
5	27.148094560619747	35.0	26.0	37.0	0.0	37.0
6	31.36097577183661	35.0	26.0	37.0	17.0	37.0
7	14.942632928674776	0.0	0.0	32.0	0.0	37.0
8	25.071725955840968	17.0	17.0	35.0	17.0	37.0
9	33.229432038204436	32.0	32.0	37.0	28.0	39.0
10	36.011247675403226	37.0	35.0	39.0	32.0	39.0
11	37.00764310303338	39.0	37.0	39.0	34.0	39.0
12	36.99552348358762	39.0	35.0	39.0	33.0	39.0
13	36.515187029983394	39.0	35.0	39.0	33.0	39.0
14	37.820187216253196	40.0	36.0	41.0	33.0	41.0
15	38.100754845783705	40.0	36.0	41.0	33.0	41.0
16	38.16936053073804	40.0	36.0	41.0	33.0	41.0
17	38.184851130154264	40.0	36.0	41.0	34.0	41.0
18	38.17451768707277	40.0	36.0	41.0	34.0	41.0
19	38.10868792558874	40.0	36.0	41.0	34.0	41.0
20	38.12705714858188	40.0	36.0	41.0	34.0	41.0
21	37.989763215842395	40.0	36.0	41.0	34.0	41.0
22	37.914437721950534	40.0	35.0	41.0	34.0	41.0
23	37.82113568194381	40.0	35.0	41.0	33.0	41.0
24	37.67759919370348	40.0	35.0	41.0	33.0	41.0
25	37.474781586072254	39.0	35.0	41.0	33.0	41.0
26	37.2258586786121	39.0	35.0	40.0	33.0	41.0
27	37.02703630650142	39.0	35.0	40.0	32.0	41.0
28	37.054000118809924	39.0	35.0	40.0	32.0	41.0
29	37.00631303596618	39.0	35.0	40.0	32.0	41.0
30	36.810655236748914	38.0	35.0	40.0	32.0	41.0
31	36.598748669681214	38.0	35.0	40.0	31.0	41.0
32	36.36496395931062	38.0	35.0	40.0	31.0	41.0
33	36.17668546481364	38.0	35.0	40.0	30.0	41.0
34	35.93176584778435	38.0	35.0	40.0	30.0	41.0
35	35.77072906000203	38.0	35.0	40.0	30.0	41.0
36	35.559309815008916	38.0	35.0	40.0	29.0	41.0
37	35.43242987445415	38.0	35.0	40.0	28.0	41.0
38	35.273914273603154	38.0	34.0	40.0	28.0	41.0
39	35.2164132893938	38.0	34.0	40.0	27.0	41.0
40	34.976823002407414	38.0	34.0	40.0	27.0	41.0
41	34.810658257340286	37.0	34.0	40.0	26.0	40.0
42	34.80279163054543	37.0	34.0	40.0	26.0	41.0
43	34.690569613052176	37.0	34.0	40.0	26.0	41.0
44	34.45505108323441	37.0	34.0	40.0	25.0	40.0
45	34.18470815549602	36.0	33.0	39.0	24.0	40.0
46	34.16822277465482	36.0	33.0	39.0	24.0	40.0
47	34.00616804758035	36.0	33.0	39.0	24.0	40.0
48	33.952791177456724	36.0	33.0	39.0	24.0	40.0
49	33.83920083207224	36.0	33.0	39.0	24.0	40.0
50	33.92018892792164	36.0	33.0	39.0	24.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
11	1.0
12	2.0
13	5.0
14	4.0
15	15.0
16	43.0
17	178.0
18	376.0
19	777.0
20	1335.0
21	2319.0
22	3741.0
23	5534.0
24	8325.0
25	12206.0
26	15296.0
27	16910.0
28	17519.0
29	19862.0
30	24557.0
31	32552.0
32	44882.0
33	82013.0
34	83114.0
35	103642.0
36	134846.0
37	248535.0
38	107727.0
39	26867.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	32.66628607215387	23.701472941039064	28.139023724731494	15.49321726207557
2	33.337260102116126	24.571000510479944	29.767726592178885	12.32401279522505
3	29.88824818789689	23.517820985659238	33.51849558439885	13.075435242045023
4	26.594897415682713	26.988983903268583	31.39753700979578	15.018581671252932
5	17.433846531807333	47.687384902882954	23.618104619189012	11.260663946120705
6	19.853742965797842	41.01137454024082	28.67850134365973	10.456381150301606
7	39.2266077852722	55.91708678058323	3.894146395981405	0.9621590381631583
8	88.1155839356896	2.7458182429622737	7.440824097875215	1.6977737234729149
9	84.18237122463836	3.980535309202836	9.321947717590817	2.515145748567988
10	48.904381166411426	23.367798280880763	15.497546776374547	12.230273776333265
11	42.80782091517877	20.639801527009624	21.630555496821835	14.921822060989767
12	38.76204083235416	20.70111953184861	25.563868894252117	14.972970741545113
13	22.174362630049043	36.34154028008937	25.650861925747826	15.833235164113763
14	16.495751538236156	38.507203606988845	28.998885401783962	15.99815945299104
15	15.20837549575456	22.76841226642019	45.52756138596815	16.49565085185711
16	17.232775832852557	17.635118603520198	43.975380166595684	21.156725397031565
17	17.25855154588832	18.00282525979603	30.701592757830127	34.03703043648552
18	21.688349478394215	22.14758005322282	34.370906469401916	21.793163998981054
19	28.322071561837042	24.284346389336104	25.90348405077413	21.490097998052725
20	30.248201992986186	22.162683010079714	25.796857175364458	21.792257821569642
21	23.12141871135531	26.32727302017856	28.832450817220995	21.71885745124514
22	22.564119603335943	22.71253132604968	26.316600263999685	28.40674880661469
23	20.48031430260083	27.91529859049138	25.92986388208417	25.67452322482362
24	20.54253848485123	23.13058117184849	38.25639383678537	18.070486506514914
25	20.00527596626201	23.336384130618427	34.69682827837367	21.961511624745896
26	18.200170562726104	30.868631460667366	27.952049118843153	22.979148857763374
27	18.614998444395443	30.85967037293228	29.146995065360564	21.378336117311715
28	17.24727467143517	26.299483579561873	37.215397363829226	19.23784438517373
29	17.762889618529513	23.48942742676828	36.36036863297096	22.387314321731242
30	20.526932096099106	27.36454409711	32.155202012116604	19.953321794674295
31	28.596542631116318	25.792527661065485	25.91234445213017	19.698585255688027
32	29.207104833650998	25.28748478377097	26.602650266869247	18.902760115708787
33	27.759838821244426	26.081296196169284	24.99186957489204	21.16699540769425
34	20.6217786651604	26.65158384708558	28.0641130587213	24.662524429032718
35	21.32164968590884	24.74186529572093	30.75626546165208	23.18021955671815
36	29.066143902986656	25.47939302223256	25.380921743525615	20.073541331255164
37	21.107691130436184	30.917766413641797	27.732250753385827	20.24229170253619
38	22.19943353843149	30.480284096687114	25.059933567127107	22.26034879775429
39	21.416999686865363	29.835387838897766	25.993598360020258	22.754014114216613
40	23.83941328033202	24.828354895321407	27.354978891100636	23.97725293324594
41	20.039408648758585	23.337390994408885	27.58011363464739	29.043086722185134
42	22.549822137511416	26.060353429327726	24.15939459294007	27.230429840220786
43	22.47058195720225	25.805314831204317	26.121671434166714	25.602431777426716
44	21.40380977121034	28.682327426063477	27.69197620176745	22.221886600958733
45	19.021267983845878	33.94147906277091	24.14378820418795	22.893464749195264
46	22.40251796496718	29.99125035366091	27.018082266812865	20.588149414559048
47	22.59321796688022	27.63357810192079	26.661451112232086	23.111752818966895
48	22.119790612606135	25.020565192920134	29.548733717753926	23.310910476719798
49	21.3338327377734	24.86611228746364	31.19183473740489	22.608220237358072
50	20.23846562013244	29.978966615417303	27.80122092303231	21.981346841417945
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	519.0
1	936.5
2	1354.0
3	6120.0
4	10886.0
5	8230.5
6	5575.0
7	5612.5
8	5650.0
9	5931.0
10	6212.0
11	6037.5
12	5863.0
13	5638.5
14	5414.0
15	5088.5
16	4763.0
17	4528.5
18	4294.0
19	4072.0
20	3850.0
21	3941.5
22	4033.0
23	4368.5
24	4704.0
25	5998.5
26	7293.0
27	8214.0
28	9135.0
29	10943.0
30	12751.0
31	15167.0
32	17583.0
33	19996.5
34	22410.0
35	24223.0
36	26036.0
37	29835.0
38	33634.0
39	45514.5
40	57395.0
41	69317.5
42	81240.0
43	84262.5
44	87285.0
45	88151.0
46	89017.0
47	87017.0
48	85017.0
49	80605.0
50	76193.0
51	67802.5
52	59412.0
53	55054.5
54	50697.0
55	48108.0
56	45519.0
57	43379.5
58	41240.0
59	38551.0
60	35862.0
61	31765.5
62	27669.0
63	23802.0
64	19935.0
65	16975.5
66	14016.0
67	12531.0
68	11046.0
69	9831.5
70	8617.0
71	6902.5
72	5188.0
73	3939.0
74	2690.0
75	2117.0
76	1544.0
77	1214.0
78	884.0
79	649.5
80	415.0
81	303.0
82	191.0
83	139.0
84	87.0
85	63.0
86	39.0
87	26.5
88	14.0
89	11.5
90	9.0
91	5.5
92	2.0
93	1.0
94	0.0
95	0.5
96	1.0
97	0.5
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
50	993183.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	23.652534805346896
#Duplication Level	Percentage of deduplicated	Percentage of total
1	70.5768110734502	16.69320480365173
2	6.604057440763854	3.124053969483544
3	3.2772870889989663	2.325484408189862
4	2.2317908440772016	2.111500424711622
5	1.7503842520083437	2.070051222167922
6	1.4042475502867324	1.9928408435088034
7	1.192370974467249	1.9741817182130403
8	0.9806117078396277	1.8555162040165964
9	0.9123677098149535	1.9421828110505543
>10	10.283003084220537	51.8925210743292
>50	0.7192736027245025	10.718075500038521
>100	0.06593728309171586	2.434378646005214
>500	0.0	0.0
>1k	0.0018573882561046722	0.866008374633481
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CGTTTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	3415	0.3438439844419407	No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATA	1594	0.16049408819925431	No Hit
CGTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1490	0.15002270477847487	No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1386	0.1395513213576954	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.006645301017033115	0.0
2	0.0	0.0	0.0	0.02708463596336224	0.0
3	0.0	0.0	0.0	0.03735464662604978	0.0
4	0.0	0.0	0.0	0.05477339020100022	0.0
5	0.0	0.0	0.0	0.09474588268224486	0.0
6	0.0	0.0	0.0	0.17559704505614776	0.0
7	0.0	0.0	0.0	0.22755121664386121	0.0
8	0.0	0.0	0.0	0.5236698574180186	0.0
9	0.0	0.0	0.0	0.6507360677740155	0.0
10	0.0	0.0	0.0	0.8478799979459979	0.0
11	0.0	0.0	0.0	1.0103878137261713	0.0
12	0.0	0.0	0.0	1.1576919862704054	0.0
13	0.0	0.0	0.0	1.2152845950846924	0.0
14	0.0	0.0	0.0	1.2341129479662862	0.0
15	0.0	0.0	0.0	1.2676415121885896	0.0
16	0.0	0.0	0.0	1.3435590419892407	0.0
17	0.0	0.0	0.0	1.4272294229764304	0.0
18	0.0	0.0	0.0	1.5711102586331018	0.0
19	0.0	0.0	0.0	1.6264877671083777	0.0
20	0.0	0.0	0.0	1.69042361780256	0.0
21	0.0	0.0	0.0	1.7704692891440954	0.0
22	0.0	0.0	0.0	1.8554485930588824	0.0
23	0.0	0.0	0.0	1.957947326927666	0.0
24	0.0	0.0	0.0	2.035576525172098	0.0
25	0.0	0.0	0.0	2.091356779163558	0.0
26	0.0	0.0	0.0	2.1482445833245234	0.0
27	0.0	0.0	0.0	2.212885238672027	0.0
28	0.0	0.0	0.0	2.277727266777623	0.0
29	0.0	0.0	0.0	2.35424891485255	0.0
30	0.0	0.0	0.0	2.4472831290910135	0.0
31	0.0	0.0	0.0	2.531759001110571	0.0
32	0.0	0.0	0.0	2.6110998677987842	0.0
33	0.0	0.0	0.0	2.687722202252757	0.0
34	0.0	0.0	0.0	2.7716946423770845	0.0
35	0.0	0.0	0.0	2.885168191561877	0.0
36	0.0	0.0	0.0	2.9768934828727436	0.0
37	0.0	0.0	0.0	3.0625775914408524	0.0
38	0.0	0.0	0.0	3.1635660296239463	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
AACGTAT	20	7.855964E-4	44.0	43
CGTATGG	75	0.0	44.0	2
CAACGGC	30	2.5273675E-6	44.0	36
CGCATAT	25	4.4424025E-5	44.0	17
TACGGGT	50	2.7284841E-11	44.0	4
TACGATT	20	7.855964E-4	44.0	35
TCCGTGA	20	7.855964E-4	44.0	33
CGTTTTA	2305	0.0	43.427334	1
TTTACGG	145	0.0	42.482758	2
TAGGCGA	265	0.0	42.339622	6
TTACGGG	250	0.0	42.24	3
CGGTCTA	220	0.0	42.0	31
ACGGTCT	220	0.0	42.0	30
CGCATCG	105	0.0	41.904762	21
AGGCGAT	885	0.0	41.762714	7
CGTTATT	1165	0.0	41.356224	1
ACGGGTA	140	0.0	40.857143	5
AATAGGC	195	0.0	40.615387	4
TATACGG	60	3.6379788E-12	40.333332	2
CGACGGT	230	0.0	40.17391	28
>>END_MODULE