##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1545719_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	382149
Sequences flagged as poor quality	0
Sequence length	51
%GC	45
>>END_MODULE
>>Per base sequence quality	fail
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	30.98729553132417	31.0	31.0	33.0	30.0	33.0
2	31.144561414526795	31.0	31.0	33.0	30.0	34.0
3	31.099995028117306	31.0	31.0	34.0	28.0	34.0
4	35.08300165642197	35.0	35.0	37.0	32.0	37.0
5	32.76831026641441	35.0	35.0	37.0	28.0	37.0
6	33.96832387367231	35.0	35.0	37.0	28.0	37.0
7	15.276779476068235	0.0	0.0	35.0	0.0	37.0
8	24.889106081659246	17.0	17.0	35.0	17.0	37.0
9	32.71588307178614	32.0	32.0	37.0	27.0	39.0
10	35.44123365493564	37.0	34.0	37.0	32.0	39.0
11	36.43796529625879	37.0	35.0	39.0	32.0	39.0
12	36.289405441333095	37.0	35.0	39.0	32.0	39.0
13	35.9761846818911	37.0	35.0	39.0	31.0	39.0
14	36.99307076559143	39.0	35.0	40.0	31.0	41.0
15	37.3507218388639	39.0	36.0	41.0	32.0	41.0
16	37.40368285668679	39.0	35.0	41.0	32.0	41.0
17	37.35575914106801	39.0	35.0	41.0	32.0	41.0
18	37.28454869697422	39.0	35.0	40.0	32.0	41.0
19	37.22294707038354	39.0	36.0	40.0	32.0	41.0
20	37.316742945814326	39.0	35.0	40.0	32.0	41.0
21	37.17096211163708	39.0	35.0	40.0	32.0	41.0
22	37.225105914185306	39.0	35.0	41.0	32.0	41.0
23	37.22168054868651	39.0	35.0	41.0	32.0	41.0
24	37.02774310543793	39.0	35.0	40.0	32.0	41.0
25	36.76482994852793	38.0	35.0	40.0	31.0	41.0
26	36.64263938934813	38.0	35.0	40.0	31.0	41.0
27	36.55170103807677	38.0	35.0	40.0	31.0	41.0
28	36.491376400304596	38.0	35.0	40.0	31.0	41.0
29	36.47388060677903	38.0	35.0	40.0	31.0	41.0
30	36.20998092367113	38.0	35.0	40.0	30.0	41.0
31	36.05431651005236	38.0	35.0	40.0	30.0	41.0
32	35.94929726363277	38.0	34.0	40.0	30.0	41.0
33	35.770042575016554	38.0	34.0	40.0	29.0	41.0
34	35.60674239629045	38.0	34.0	40.0	28.0	41.0
35	35.420532305462004	38.0	34.0	40.0	27.0	41.0
36	35.19965510834779	38.0	34.0	40.0	26.0	41.0
37	35.05509107704063	38.0	34.0	40.0	25.0	41.0
38	34.97841156198237	38.0	34.0	40.0	25.0	41.0
39	34.880619862933045	38.0	34.0	40.0	25.0	41.0
40	34.70380401361773	38.0	33.0	40.0	24.0	41.0
41	34.60116342055062	37.0	33.0	40.0	24.0	41.0
42	34.53731397962575	37.0	33.0	40.0	23.0	41.0
43	34.450839855658394	37.0	33.0	40.0	23.0	41.0
44	34.20145021967871	37.0	33.0	40.0	23.0	41.0
45	34.110101033889926	37.0	33.0	40.0	23.0	41.0
46	34.109357868266045	37.0	33.0	40.0	23.0	41.0
47	34.042211284080295	37.0	33.0	40.0	23.0	41.0
48	34.00057569168047	36.0	33.0	40.0	23.0	41.0
49	33.90952481885338	36.0	33.0	40.0	23.0	41.0
50	33.77796880274448	36.0	33.0	40.0	23.0	41.0
51	33.57788977597743	36.0	32.0	40.0	22.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
9	1.0
10	0.0
11	1.0
12	0.0
13	1.0
14	5.0
15	21.0
16	62.0
17	111.0
18	229.0
19	476.0
20	812.0
21	1338.0
22	2031.0
23	3034.0
24	4208.0
25	5662.0
26	6744.0
27	7298.0
28	8194.0
29	9174.0
30	11255.0
31	14539.0
32	19064.0
33	28313.0
34	35948.0
35	38147.0
36	45048.0
37	68416.0
38	59927.0
39	12090.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	32.40830147403238	24.375309107180705	28.14975310677249	15.066636312014422
2	32.96358226764953	24.373215682888087	30.339998272924955	12.323203776537424
3	30.42425860070287	23.22863595089873	33.13184124516877	13.21526420322963
4	26.963304888930757	27.57275303611942	30.250504384415507	15.21343769053432
5	21.235957702362168	36.11313911589459	28.64353956179396	14.007363619949286
6	19.830223289868613	41.7674781302581	28.197901865502722	10.204396714370572
7	38.266226000853074	56.82704913528492	4.1735030053722495	0.7332218584897514
8	88.64212650039644	2.987578143603673	6.786619878633726	1.583675477366158
9	84.53011783361987	4.122475788239665	8.665991537332298	2.6814148408081664
10	49.00837108039011	23.960026063132442	14.985777798712022	12.045825057765427
11	43.205398941250664	21.016409829673766	20.951251998566004	14.826939230509565
12	39.10909095666874	21.120557688231553	25.05541032424525	14.714941030854456
13	22.315117925207183	36.99080725057504	24.972981742723388	15.721093081494391
14	16.360110846816294	39.33910595082023	28.462720038519006	15.838063163844469
15	15.180727935962151	23.044414613148277	45.39904592187864	16.375811529010935
16	17.117930440744317	17.851937333343802	43.846248452828604	21.183883773083274
17	17.208471041400085	18.244454388209835	30.23689712651348	34.310177443876604
18	21.83938725470955	22.427377802898867	34.183786952209736	21.549447990181843
19	28.871461131652836	24.55011003561438	25.251407173641695	21.327021659091088
20	30.761823267887657	22.464274406056276	25.251407173641695	21.522495152414372
21	23.04781642762378	26.920913047005225	28.419804840520317	21.61146568485067
22	22.177475277967496	23.009873112320065	25.838351009684708	28.974300600027735
23	20.367709976998498	28.49124294450594	25.524599043828456	25.616448034667105
24	20.356196143389095	23.436408311941154	38.405177038275646	17.8022185063941
25	20.017846442094577	23.68918929527488	34.60037838644089	21.692585876189654
26	18.1350729689205	31.568053298582488	27.409465941295146	22.88740779120186
27	18.39256415691262	31.743900939162472	28.705033900389637	21.15850100353527
28	17.189630222766514	26.894221887274334	36.97877006089248	18.937377829066673
29	17.579007141193618	23.97232493085158	36.157624382112736	22.29104354584207
30	20.607668736539935	27.97652224655828	31.827637910867228	19.588171106034558
31	29.14125118736409	26.239765117794366	25.314994936529995	19.30398875831155
32	29.823707506757835	25.29249062538434	26.37819279914379	18.505609068714037
33	28.245527268159805	26.089823602835544	24.66236991330607	21.00227921569859
34	20.484680059348577	26.62809532407516	27.724526297334286	25.162698319241972
35	21.246948179898418	24.71470552062154	30.827242777031994	23.211103522448052
36	29.485619483499892	25.671923778421508	25.167670201936943	19.67478653614166
37	20.89629961088476	31.743639261125896	27.484305859756276	19.87575526823307
38	21.817144621600477	31.182601550704042	24.849469709458877	22.150784118236604
39	21.38328243695522	30.31199872301118	25.70620360121314	22.59851523882046
40	24.032249201227792	24.869880596311912	27.207712175094006	23.89015802736629
41	19.71927180235981	23.587919895119445	27.39219519088104	29.300613111639702
42	22.36091158160822	26.08040319351876	23.956885926693513	27.601799298179508
43	22.173288429382257	25.549458457303302	26.286867164378293	25.990385948936144
44	20.97846651437005	28.646941376269464	28.153939955357725	22.220652154002757
45	19.01012432323518	34.65297567179294	23.914494084767984	22.4224059202039
46	22.220652154002757	30.666310784536922	26.61187128580737	20.50116577565295
47	22.437844924361965	27.856412027769274	26.243428610306452	23.462314437562313
48	21.947460283815996	25.31420990242026	29.83495966233066	22.90337015143308
49	21.165304632486283	25.1046057951218	31.426485480794142	22.30360409159778
50	20.160722650065814	30.833261371873277	27.49058613263413	21.515429845426784
51	20.199712677515837	31.39220565800251	25.918424488877374	22.48965717560428
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	200.0
1	358.5
2	517.0
3	1972.0
4	3427.0
5	2664.5
6	1902.0
7	1937.0
8	1972.0
9	1995.5
10	2019.0
11	1993.5
12	1968.0
13	1909.0
14	1850.0
15	1728.0
16	1606.0
17	1588.0
18	1570.0
19	1481.5
20	1393.0
21	1418.5
22	1444.0
23	1597.0
24	1750.0
25	2230.0
26	3079.5
27	3449.0
28	3865.5
29	4282.0
30	5106.0
31	5930.0
32	7173.0
33	8416.0
34	9168.0
35	9920.0
36	11163.0
37	12406.0
38	15983.0
39	19560.0
40	24141.0
41	28722.0
42	30857.0
43	32992.0
44	33236.0
45	33480.0
46	32861.0
47	32242.0
48	30763.5
49	29285.0
50	27406.5
51	25528.0
52	23155.0
53	20782.0
54	19691.5
55	18601.0
56	17504.0
57	16407.0
58	15436.0
59	14465.0
60	12967.5
61	11470.0
62	10070.0
63	8670.0
64	7465.5
65	6261.0
66	5468.5
67	4676.0
68	4202.5
69	3729.0
70	3191.5
71	2654.0
72	2239.5
73	1825.0
74	1442.5
75	754.5
76	449.0
77	361.5
78	274.0
79	208.0
80	142.0
81	110.5
82	79.0
83	56.5
84	34.0
85	27.0
86	20.0
87	14.5
88	9.0
89	5.0
90	1.0
91	1.0
92	1.0
93	0.5
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	382149.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	34.12324033882611
#Duplication Level	Percentage of deduplicated	Percentage of total
1	67.57299555206578	23.058095676375682
2	8.598020734910284	5.867846559511078
3	4.724553344550163	4.836512078090697
4	3.2579546909616264	4.4468788373075805
5	2.407955910992637	4.108362913804936
6	2.0081713270540757	4.111518770076335
7	1.5922334308078496	3.8032514824478927
8	1.3378841800916315	3.6522354738224045
9	1.1403231136286058	3.5020367703240693
>10	7.295120003995215	40.2760909734768
>50	0.04954354366161891	1.1022622334368606
>100	0.012957542188423408	0.6338397977549857
>500	0.002286625092074719	0.6010684335706902
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CGTTTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	874	0.22870660396860912	No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	816	0.21352927784712245	No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATAC	621	0.16250206071453804	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.006018594841279187	0.0
2	0.0	0.0	0.0	0.024859413474848817	0.0
3	0.0	0.0	0.0	0.03454150082821099	0.0
4	0.0	0.0	0.0	0.05364399749835797	0.0
5	0.0	0.0	0.0	0.0921106688752293	0.0
6	0.0	0.0	0.0	0.14758641262962877	0.0
7	0.0	0.0	0.0	0.17924945505548884	0.0
8	0.0	0.0	0.0	0.41135787349960357	0.0
9	0.0	0.0	0.0	0.5223093610084025	0.0
10	0.0	0.0	0.0	0.6874282020887141	0.0
11	0.0	0.0	0.0	0.81748218626766	0.0
12	0.0	0.0	0.0	0.9368073709469343	0.0
13	0.0	0.0	0.0	0.9857411637868999	0.0
14	0.0	0.0	0.0	1.0045819824204696	0.0
15	0.0	0.0	0.0	1.030749786078205	0.0
16	0.0	0.0	0.0	1.0870105639423366	0.0
17	0.0	0.0	0.0	1.1602804141839962	0.0
18	0.0	0.0	0.0	1.3015865539357685	0.0
19	0.0	0.0	0.0	1.353398805178085	0.0
20	0.0	0.0	0.0	1.411491329298258	0.0
21	0.0	0.0	0.0	1.4740323800402462	0.0
22	0.0	0.0	0.0	1.5428537036600907	0.0
23	0.0	0.0	0.0	1.6244972510722258	0.0
24	0.0	0.0	0.0	1.6875616578873684	0.0
25	0.0	0.0	0.0	1.7359720946541795	0.0
26	0.0	0.0	0.0	1.7924945505548884	0.0
27	0.0	0.0	0.0	1.8484936503824425	0.0
28	0.0	0.0	0.0	1.9086795987952343	0.0
29	0.0	0.0	0.0	1.973837429902996	0.0
30	0.0	0.0	0.0	2.0560043333882856	0.0
31	0.0	0.0	0.0	2.1321526420322963	0.0
32	0.0	0.0	0.0	2.200188931542409	0.0
33	0.0	0.0	0.0	2.265870118723325	0.0
34	0.0	0.0	0.0	2.3404483591478717	0.0
35	0.0	0.0	0.0	2.4500914564737837	0.0
36	0.0	0.0	0.0	2.5359218524711564	0.0
37	0.0	0.0	0.0	2.62070553632222	0.0
38	0.0	0.0	0.0	2.699470625332004	0.0
39	0.0	0.0	0.0	2.803880161926369	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
AGGTACG	20	7.027368E-4	45.000004	7
CTATCGA	20	7.027368E-4	45.000004	38
CGCATCG	30	2.1616834E-6	45.000004	21
GTTAACG	20	7.027368E-4	45.000004	1
TTAACGG	30	2.1616834E-6	45.000004	2
TCGTACA	30	2.1616834E-6	45.000004	34
CTCGATA	20	7.027368E-4	45.000004	1
CGGTAAT	20	7.027368E-4	45.000004	25
TCTATCG	20	7.027368E-4	45.000004	37
TCTTCAC	25	3.885902E-5	45.0	37
GTTAGGC	50	2.1827873E-11	45.0	4
CGATTGT	25	3.885902E-5	45.0	10
TCGATAG	45	3.8380676E-10	45.0	2
AGTATGA	25	3.885902E-5	45.0	1
GCCGATG	25	3.885902E-5	45.0	9
TGATTCG	25	3.885902E-5	45.0	15
CATCGTT	25	3.885902E-5	45.0	36
AGGATCG	25	3.885902E-5	45.0	7
TAGGACA	25	3.885902E-5	45.0	6
ATCGCGA	25	3.885902E-5	45.0	1
>>END_MODULE