FastQCFastQC Report
Thu 26 May 2016
SRR1545718_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1545718_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences1614569
Sequences flagged as poor quality0
Sequence length50
%GC41

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT207711.2864733560473414No Hit
CGTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT89630.5551326700809938No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCGATA207.85743E-444.01
CGTTATG207.85743E-444.01
CGTTTTA122050.043.8197481
CGTTATT69250.043.332851
GTTATTT72550.042.2715382
GTTTTAT130650.042.029852
CGTTTTT58000.041.458621
CGACGGT2800.040.0714328
CGCTAAG501.3478711E-939.6000022
AGGCGAT10500.039.67
AGGATGC10150.039.0147827
AGGTACC3850.038.2857137
CGGTCTA3000.038.1333331
CGTAAAG1100.038.02
CGTTGAT2500.037.83999625
TAACGGG3200.037.81253
ATAGGAT8800.037.755
ACGGGAT5600.037.7142875
ATAACGG1050.037.7142872
ATAGGCG2050.037.5609745
Produced by FastQC (version 0.11.5)