##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1545718_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	1614569
Sequences flagged as poor quality	0
Sequence length	50
%GC	41
>>END_MODULE
>>Per base sequence quality	fail
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.48907045781258	31.0	31.0	33.0	30.0	34.0
2	31.686123045840716	31.0	31.0	34.0	30.0	34.0
3	31.56008879149792	31.0	31.0	34.0	30.0	34.0
4	35.540637160753114	37.0	35.0	37.0	33.0	37.0
5	26.895489756089706	35.0	19.0	37.0	0.0	37.0
6	31.227077319086394	35.0	25.0	37.0	17.0	37.0
7	15.234928330718601	0.0	0.0	32.0	0.0	37.0
8	25.24228261536051	17.0	17.0	35.0	17.0	37.0
9	33.27848422705998	32.0	32.0	37.0	28.0	39.0
10	36.02730016493566	37.0	35.0	39.0	32.0	39.0
11	36.999417801283194	39.0	37.0	39.0	34.0	39.0
12	37.247527978054826	39.0	37.0	39.0	34.0	39.0
13	37.00235604672206	39.0	37.0	39.0	33.0	39.0
14	38.408838519753566	40.0	38.0	41.0	34.0	41.0
15	38.58656087166296	40.0	38.0	41.0	34.0	41.0
16	38.55434422437195	40.0	38.0	41.0	34.0	41.0
17	38.52321145767075	40.0	38.0	41.0	34.0	41.0
18	38.445755492642306	40.0	38.0	41.0	34.0	41.0
19	38.362530805434766	40.0	37.0	41.0	34.0	41.0
20	38.339532717400125	40.0	37.0	41.0	34.0	41.0
21	38.19448162326912	40.0	37.0	41.0	34.0	41.0
22	37.97359481075135	39.0	36.0	41.0	34.0	41.0
23	37.76234772251914	39.0	36.0	41.0	33.0	41.0
24	37.51536354284023	39.0	35.0	41.0	33.0	41.0
25	37.336038286378596	39.0	35.0	41.0	33.0	41.0
26	37.05195318379084	39.0	35.0	40.0	33.0	41.0
27	36.84417327472533	39.0	35.0	40.0	32.0	41.0
28	36.823061758277284	38.0	35.0	40.0	32.0	41.0
29	36.67200782375978	38.0	35.0	40.0	32.0	41.0
30	36.364507803630566	38.0	35.0	40.0	31.0	41.0
31	36.07202727167436	38.0	35.0	40.0	30.0	41.0
32	35.614815470877986	38.0	35.0	40.0	27.0	41.0
33	35.094709485937116	38.0	34.0	40.0	24.0	41.0
34	34.52186682637905	38.0	34.0	40.0	21.0	41.0
35	34.07027757872225	38.0	33.0	40.0	18.0	41.0
36	33.79939414171832	38.0	33.0	40.0	15.0	41.0
37	33.679353437356966	38.0	33.0	40.0	15.0	41.0
38	33.482440824765	38.0	33.0	40.0	15.0	41.0
39	33.35029967749907	38.0	33.0	40.0	13.0	41.0
40	33.12449762134663	37.0	33.0	40.0	12.0	41.0
41	32.86616799901398	37.0	32.0	40.0	10.0	40.0
42	32.848455532095564	37.0	32.0	40.0	10.0	40.0
43	32.70356423293151	37.0	32.0	40.0	10.0	40.0
44	32.4468040696929	37.0	31.0	40.0	10.0	40.0
45	32.291571310981446	36.0	31.0	39.0	10.0	40.0
46	32.206972263186024	36.0	31.0	39.0	10.0	40.0
47	32.05105449194181	36.0	31.0	39.0	10.0	40.0
48	31.91305419588757	36.0	31.0	39.0	10.0	40.0
49	31.76064695903365	35.0	31.0	39.0	10.0	40.0
50	31.883515043333546	35.0	31.0	39.0	10.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
10	3.0
11	0.0
12	2.0
13	7.0
14	17.0
15	45.0
16	128.0
17	335.0
18	897.0
19	2007.0
20	3606.0
21	6346.0
22	10492.0
23	17024.0
24	27081.0
25	40660.0
26	49883.0
27	48135.0
28	42182.0
29	38558.0
30	40970.0
31	49047.0
32	63192.0
33	97231.0
34	115221.0
35	153442.0
36	219715.0
37	384768.0
38	157995.0
39	45580.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	30.632695165087405	20.67053188807663	24.247337834431356	24.44943511240461
2	42.919813275245595	20.698898591512656	25.20449729927925	11.176790833962501
3	27.266967221592886	21.227212959000205	40.31379272115345	11.192027098253465
4	23.58809069169543	23.995382049327098	39.30225341871422	13.114273840263252
5	15.413091667187961	45.77469281275684	28.886718374996672	9.925497145058525
6	17.90521185530008	36.36580412481597	36.57180337291253	9.15718064697142
7	35.29059457972995	54.62869657475152	9.210383699922394	0.8703251455961313
8	77.67199791399439	2.3927128540186264	18.29862954138225	1.6366596906047373
9	73.37822044149243	4.227134300237401	19.654347383109673	2.740297875160492
10	33.664959503124365	28.974357862686574	25.816796928468218	11.543885705720845
11	26.9428559572245	22.500865556071002	35.770970457131284	14.785308029573219
12	25.84460620760091	20.16761129440736	38.636193312270954	15.351589185720771
13	21.8102168442476	23.49549632130928	38.07486703881965	16.619419795623475
14	17.929057228275784	25.986563596848445	38.62869905219288	17.455680122682896
15	17.291797377504462	22.671499328923076	42.71455725955347	17.32214603401899
16	19.88951850308039	20.34127993291089	41.48041985198527	18.288781712023454
17	19.665743613311047	20.922735417315703	38.36900126287573	21.042519706497522
18	20.930601293596	22.136495869795592	38.59952718031871	18.333375656289697
19	22.975171702169433	24.033348837987102	35.06595258548876	17.925526874354706
20	24.378642225881954	22.76632339652254	35.273562170461595	17.581472207133917
21	22.40294468678638	23.967510834160695	36.6829166173759	16.946627861677015
22	21.262330690109867	21.207517300282614	36.79440147804151	20.73575053156601
23	19.97895413574768	23.92657111588294	36.63516393539081	19.459310812978572
24	19.847835552398195	23.320465090064282	39.713756426637694	17.117942930899826
25	20.651455589696074	23.431949950729887	37.02752870890002	18.88906575067402
26	19.075617084187794	25.076599389682325	35.956345006004696	19.891438520125185
27	18.61245942415592	23.629278154108	37.69761465753399	20.060647764202088
28	18.46697168098731	23.92855306896144	39.111428498874936	18.49304675117632
29	19.367149994828342	23.00737843969505	38.22630064122376	19.39917092425285
30	20.385006772705285	23.37769398520596	38.16430267148694	18.072996570601813
31	22.829931703135635	23.677092772126805	35.10249484537359	18.390480679363964
32	23.131807931404605	23.86389184977539	34.49750366816159	18.50679655065841
33	22.77771962672391	24.57795238233857	33.23815829487622	19.4061696960613
34	20.196535422146717	25.594508503507747	34.450246474446125	19.758709599899415
35	21.30060715893839	25.794004468065474	32.67615072505418	20.229237647941957
36	22.9505830967893	27.57627577390622	30.03476469571756	19.43837643358692
37	21.222134204236546	29.093089239295438	29.997045651192362	19.68773090527565
38	21.730381296804286	28.416500007122643	30.120050614126743	19.733068081946328
39	21.433645759332677	28.055660674768312	29.030224165086782	21.48046940081223
40	22.257456943617772	26.102817532109192	31.364283595188564	20.27544192908448
41	20.54554497206375	26.00520634299308	30.896109116426736	22.55313956851643
42	20.997554146029067	27.692281965032155	29.01090012257141	22.29926376636737
43	20.978415911614803	26.9428559572245	29.797797430769453	22.28093070039125
44	21.96251755112355	26.799721783336604	30.32128078762815	20.91647987791169
45	20.73457374692565	28.00741250451359	29.671881474251023	21.586132274309737
46	21.682814422920295	27.5240017614608	30.453266475449485	20.339917340169418
47	20.563134805635435	27.51025196197871	31.015645661473744	20.910967570912113
48	20.659816954245994	26.73691864516165	31.250073549040025	21.353190851552334
49	21.245298280841514	26.16710713509302	31.489208575167737	21.09838600889773
50	20.13943039907245	26.40302148746817	32.148146037735145	21.309402075724236
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	865.0
1	1774.0
2	2683.0
3	33141.0
4	63599.0
5	45085.0
6	26571.0
7	25933.0
8	25295.0
9	25108.5
10	24922.0
11	24578.5
12	24235.0
13	23524.5
14	22814.0
15	21604.5
16	20395.0
17	19214.0
18	18033.0
19	17051.5
20	16070.0
21	15326.0
22	14582.0
23	14728.5
24	14875.0
25	16049.0
26	17223.0
27	19479.0
28	21735.0
29	25159.0
30	28583.0
31	32231.0
32	35879.0
33	40525.0
34	45171.0
35	48443.0
36	51715.0
37	55506.5
38	59298.0
39	65219.0
40	71140.0
41	77915.0
42	84690.0
43	89506.5
44	94323.0
45	99143.5
46	103964.0
47	107954.5
48	111945.0
49	107557.5
50	103170.0
51	95271.5
52	87373.0
53	81606.0
54	75839.0
55	73984.5
56	72130.0
57	69348.0
58	66566.0
59	61835.5
60	57105.0
61	51529.0
62	45953.0
63	39401.0
64	32849.0
65	27986.5
66	23124.0
67	20519.0
68	17914.0
69	15918.5
70	13923.0
71	11309.5
72	8696.0
73	6632.5
74	4569.0
75	3512.5
76	2456.0
77	1842.0
78	1228.0
79	951.5
80	675.0
81	468.5
82	262.0
83	165.0
84	68.0
85	48.0
86	28.0
87	25.5
88	23.0
89	15.0
90	7.0
91	6.0
92	5.0
93	2.5
94	0.0
95	0.5
96	1.0
97	0.5
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
50	1614569.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	40.60766379501295
#Duplication Level	Percentage of deduplicated	Percentage of total
1	70.54579700970454	28.647000071213117
2	9.780703718264565	7.94343056539841
3	5.000220885847727	6.091418658999189
4	3.197777379376207	5.194170748520263
5	2.2919787388424213	4.6535951026115425
6	1.653588591305252	4.0289021742595645
7	1.3046614447274043	3.708547731966442
8	1.0108733131192054	3.2839362910796437
9	0.7903080984317228	2.888330900003226
>10	4.358545804374851	28.388345656005992
>50	0.04178046980962824	1.1412065328534302
>100	0.02290351827071215	1.828712261185357
>500	5.166167554569742E-4	0.12314437916166264
>1k	0.0	0.0
>5k	1.7220558515232474E-4	0.6267706248869859
>10k+	1.7220558515232474E-4	1.452488301855136
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CGTTTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	20771	1.2864733560473414	No Hit
CGTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	8963	0.5551326700809938	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.00532649889846764	0.0
2	0.0	0.0	0.0	0.02297826850385459	0.0
3	0.0	0.0	0.0	0.040382293974429086	0.0
4	0.0	0.0	0.0	0.05549468619798844	0.0
5	0.0	0.0	0.0	0.09884990979016692	0.0
6	0.0	0.0	0.0	0.22451812217378136	0.0
7	0.0	0.0	0.0	0.31853702133510553	0.0
8	0.0	0.0	0.0	0.7502931122795	0.0
9	0.0	0.0	0.0	1.0295626882468325	0.0
10	0.0	0.0	0.0	1.3835890568938212	0.0
11	0.0	0.0	0.0	1.5820940449122955	0.0
12	0.0	0.0	0.0	1.7683356982575535	0.0
13	0.0	0.0	0.0	1.8693533692273294	0.0
14	0.0	0.0	0.0	1.929369385885645	0.0
15	0.0	0.0	0.0	1.973529777915964	0.0
16	0.0	0.0	0.0	2.034784515248342	0.0
17	0.0	0.0	0.0	2.1053915936698897	0.0
18	0.0	0.0	0.0	2.2251139468180052	0.0
19	0.0	0.0	0.0	2.2805466969822907	0.0
20	0.0	0.0	0.0	2.3460750206401833	0.0
21	0.0	0.0	0.0	2.408382670545514	0.0
22	0.0	0.0	0.0	2.482829783056655	0.0
23	0.0	0.0	0.0	2.5652047078817937	0.0
24	0.0	0.0	0.0	2.6330866008204046	0.0
25	6.19360337031121E-5	0.0	0.0	2.6935981057483454	0.0
26	6.19360337031121E-5	0.0	0.0	2.7524373377663016	0.0
27	6.19360337031121E-5	0.0	0.0	2.8293618916255667	0.0
28	1.238720674062242E-4	0.0	0.0	2.898358633170834	0.0
29	1.238720674062242E-4	0.0	0.0	2.975221250996396	0.0
30	1.238720674062242E-4	0.0	0.0	3.0844144784149825	0.0
31	0.0011767846403591298	0.0	0.0	3.1614629043416542	0.0
32	0.0011767846403591298	0.0	0.0	3.2490404559978545	0.0
33	0.0011767846403591298	0.0	0.0	3.334264438373337	0.0
34	0.0011767846403591298	0.0	0.0	3.4297698023435355	0.0
35	0.0011767846403591298	0.0	0.0	3.521311260156735	0.0
36	0.0011767846403591298	0.0	0.0	3.6108707648914353	0.0
37	0.0011767846403591298	0.0	0.0	3.710587779153446	0.0
38	0.0011767846403591298	0.0	0.0	3.8280184990545463	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GTCGATA	20	7.85743E-4	44.0	1
CGTTATG	20	7.85743E-4	44.0	1
CGTTTTA	12205	0.0	43.819748	1
CGTTATT	6925	0.0	43.33285	1
GTTATTT	7255	0.0	42.271538	2
GTTTTAT	13065	0.0	42.02985	2
CGTTTTT	5800	0.0	41.45862	1
CGACGGT	280	0.0	40.07143	28
CGCTAAG	50	1.3478711E-9	39.600002	2
AGGCGAT	1050	0.0	39.6	7
AGGATGC	1015	0.0	39.014782	7
AGGTACC	385	0.0	38.285713	7
CGGTCTA	300	0.0	38.13333	31
CGTAAAG	110	0.0	38.0	2
CGTTGAT	250	0.0	37.839996	25
TAACGGG	320	0.0	37.8125	3
ATAGGAT	880	0.0	37.75	5
ACGGGAT	560	0.0	37.714287	5
ATAACGG	105	0.0	37.714287	2
ATAGGCG	205	0.0	37.560974	5
>>END_MODULE