FastQCFastQC Report
Thu 26 May 2016
SRR1545717_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1545717_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences610651
Sequences flagged as poor quality0
Sequence length51
%GC42

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT54580.8938002230406565No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT49620.8125754317932828No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAATCGT207.030687E-445.012
CGCATCG253.8886505E-545.021
GTTACGG253.8886505E-545.02
TTGACGA253.8886505E-545.01
GCGATAC207.030687E-445.09
CACGTTA253.8886505E-545.01
GACGATA253.8886505E-545.01
GCGCAAA302.1638243E-644.9999961
GCGTAAA302.1638243E-644.9999961
TGCACGG600.044.9999962
CGTTTTA33650.044.398221
CGTTTTT43700.044.2276881
CGTTATT6800.042.6838231
GTTTTAT36300.042.2107432
AGGCGAT4750.041.6842087
ACGGGAT1400.040.1785745
CGGTCTA1350.040.031
TAACGGG850.039.7058833
GTTATTT7550.039.635762
CTCACGA1600.039.37524
Produced by FastQC (version 0.11.5)