##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1545717_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	610651
Sequences flagged as poor quality	0
Sequence length	51
%GC	42
>>END_MODULE
>>Per base sequence quality	fail
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	30.951584456588133	31.0	31.0	33.0	30.0	33.0
2	31.13153830911601	31.0	31.0	33.0	30.0	34.0
3	30.88442989530845	31.0	31.0	34.0	27.0	34.0
4	34.83340893570959	35.0	35.0	37.0	32.0	37.0
5	32.67289007960357	35.0	35.0	37.0	28.0	37.0
6	33.93273899494147	35.0	35.0	37.0	28.0	37.0
7	16.10279030084287	0.0	0.0	35.0	0.0	37.0
8	25.331203911890753	17.0	17.0	35.0	17.0	37.0
9	32.86625748586345	32.0	32.0	37.0	27.0	39.0
10	35.50163022741304	37.0	34.0	38.0	32.0	39.0
11	36.41904131819976	37.0	35.0	39.0	32.0	39.0
12	36.54454017106334	38.0	35.0	39.0	32.0	39.0
13	36.524324041064375	38.0	35.0	39.0	32.0	39.0
14	37.654691468613	39.0	36.0	40.0	33.0	41.0
15	37.82414013896645	40.0	37.0	41.0	33.0	41.0
16	37.75218250686562	40.0	36.0	41.0	33.0	41.0
17	37.63156860465307	40.0	36.0	41.0	32.0	41.0
18	37.50837876299228	39.0	36.0	40.0	32.0	41.0
19	37.39147237947699	39.0	36.0	40.0	32.0	41.0
20	37.40882271542993	39.0	35.0	40.0	32.0	41.0
21	37.30015016760801	39.0	35.0	40.0	32.0	41.0
22	37.248145012453925	39.0	35.0	40.0	32.0	41.0
23	37.14116901470725	39.0	35.0	40.0	32.0	41.0
24	36.86401397852456	38.0	35.0	40.0	32.0	41.0
25	36.60952982964083	38.0	35.0	40.0	31.0	41.0
26	36.48383282758892	38.0	35.0	40.0	31.0	41.0
27	36.41430702643572	38.0	35.0	40.0	31.0	41.0
28	36.26022064976558	38.0	35.0	40.0	30.0	41.0
29	36.1512369585901	38.0	35.0	40.0	30.0	41.0
30	35.8007339707951	38.0	34.0	40.0	29.0	41.0
31	35.58021521294487	38.0	34.0	40.0	27.0	41.0
32	35.270902692372566	38.0	34.0	40.0	25.0	41.0
33	34.77999217228826	38.0	34.0	40.0	23.0	41.0
34	34.309119284173775	38.0	33.0	40.0	20.0	41.0
35	33.923005120764564	38.0	33.0	40.0	17.0	41.0
36	33.6471224971383	38.0	33.0	40.0	15.0	41.0
37	33.52283710335363	38.0	33.0	40.0	15.0	41.0
38	33.36544605674927	38.0	33.0	40.0	15.0	41.0
39	33.20368754001877	38.0	32.0	40.0	12.0	41.0
40	33.069632244932045	37.0	32.0	40.0	12.0	41.0
41	32.85488437749222	37.0	32.0	40.0	10.0	41.0
42	32.778562550458446	37.0	31.0	40.0	10.0	41.0
43	32.68849146239014	37.0	31.0	40.0	10.0	41.0
44	32.46406376146113	37.0	31.0	40.0	10.0	41.0
45	32.40509718317009	37.0	31.0	40.0	10.0	41.0
46	32.35250740603061	36.0	31.0	40.0	10.0	41.0
47	32.25843403187746	36.0	31.0	40.0	10.0	41.0
48	32.17248641204223	36.0	30.0	40.0	10.0	41.0
49	32.04877745225996	36.0	30.0	40.0	10.0	41.0
50	31.96952268971966	36.0	30.0	40.0	10.0	40.0
51	31.84446107514767	35.0	30.0	39.0	10.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
12	4.0
13	6.0
14	11.0
15	46.0
16	124.0
17	276.0
18	641.0
19	1219.0
20	2043.0
21	3481.0
22	5386.0
23	8165.0
24	12122.0
25	16178.0
26	18487.0
27	17713.0
28	16034.0
29	15871.0
30	17376.0
31	21145.0
32	27101.0
33	36851.0
34	46426.0
35	56275.0
36	72156.0
37	106524.0
38	88169.0
39	20821.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	30.888183266710445	21.592366179700026	24.737534205298935	22.781916348290597
2	41.73316673517279	20.980560090788355	25.807703581915035	11.478569592123815
3	27.961143107929075	21.40043985844615	39.12562167260841	11.512795361016357
4	24.360886987821196	24.82596442157632	37.30019274511955	13.512955845482935
5	19.283846255881016	33.593656605819035	34.63369420503692	12.488802933263026
6	18.264442373794523	37.47590686005591	35.25991114400861	8.999739622140961
7	35.08681718362862	54.22196966843582	10.006534010424941	0.6846791375106239
8	79.17124511382116	2.754273717720924	16.479298322609807	1.5951828458481194
9	74.66245040129304	4.5025718454567345	17.862084889732433	2.9728928635177865
10	34.01386389279637	30.116220230540847	24.263613749916075	11.606302126746701
11	27.425157741492278	23.299724392492603	34.39902661258231	14.876091253432813
12	26.151271348118648	20.903429291035305	37.488352594198645	15.456946766647398
13	22.412310796183093	24.344674781503674	36.50088184576788	16.74213257654536
14	18.168479213167586	27.203590921819497	37.1577218411171	17.470208023895808
15	17.594010326684145	23.51965361556765	41.32458638403933	17.561749673708878
16	20.165692023758254	21.15185269491084	40.09065734765029	18.591797933680613
17	19.853402352571273	21.85127020180103	36.960227691430944	21.33509975419675
18	21.18591470414361	22.843981259344538	37.43594950307131	18.534154533440542
19	23.37505383598815	24.72656230809415	33.64949864980161	18.248885206116093
20	25.01265043371746	23.38111294339975	33.91495305829353	17.691283564589266
21	22.77700355849741	24.83284232728678	35.2623675389052	17.127786575310612
22	21.491326469620127	21.85913066547013	35.62427638700338	21.025266477906364
23	20.333381915365734	24.643208641269727	35.352599111440085	19.670810331924454
24	19.988831591203486	24.15061958467275	38.77419344273571	17.08635538138806
25	20.904903127973263	24.236429646393766	35.80506705139269	19.05360017424028
26	19.34705748455337	25.957216151287728	34.71082500478997	19.984901359368937
27	18.92472132199898	24.551503231796886	36.26719681127191	20.25657863493223
28	18.63126401168589	24.90538785656619	37.77771591301742	18.685632218730504
29	19.501482843719245	23.75170105346589	37.145767385953675	19.601048716861186
30	20.539555327019855	24.298985836427025	36.99068698814871	18.170771848404407
31	23.331166247169005	24.440310422811066	33.73252479730648	18.495998532713447
32	23.58794139369296	24.398387949909196	33.37045218954853	18.643218466849312
33	23.217025764307273	25.073569027152985	32.12669757357312	19.582707634966617
34	20.44211832945496	25.946080494423164	33.49736592587255	20.114435250249326
35	21.504591002061733	26.105091124062678	32.12096598548107	20.269351888394517
36	23.174284493106537	28.05006460318578	29.27547813726662	19.50017276644106
37	21.247160816898685	29.575322074310854	29.582855018660414	19.59466209013004
38	21.70437778698471	28.754886178848476	29.68847999921395	19.852256034952863
39	21.46086717290236	28.51776219149727	28.5272602517641	21.494110383836265
40	22.38938444381488	26.388559095129626	30.918970082747755	20.303086378307743
41	20.453581505639065	26.38986917240781	30.484679465029945	22.671869856923184
42	21.01363954206249	28.064475453245798	28.447509297454683	22.47437570723703
43	21.050976744490715	27.196385496789492	29.34343839607239	22.409199362647403
44	21.971142272754815	27.110411675408702	30.051862684250086	20.866583367586394
45	20.783884739401064	28.53086296427911	29.024107059515174	21.661145236804654
46	21.692914610800603	28.076921187388542	29.958519678179517	20.271644523631338
47	20.523998159341424	28.084290372078325	30.213493468445968	21.178218000134283
48	20.640758796759524	27.2332314202384	30.80908735104012	21.316922431961956
49	21.28662689490396	26.6040668073908	31.15494775248055	20.954358545224686
50	20.209252093257852	27.182957204688112	31.473787810058447	21.13400289199559
51	19.784787055126415	27.619540457642742	30.90390419404865	21.691768293182196
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	360.0
1	679.5
2	999.0
3	10331.0
4	19663.0
5	14210.0
6	8757.0
7	8627.0
8	8497.0
9	8400.0
10	8303.0
11	8262.5
12	8222.0
13	8008.0
14	7794.0
15	7334.0
16	6874.0
17	6677.5
18	6481.0
19	6049.5
20	5618.0
21	5538.0
22	5458.0
23	5507.5
24	5557.0
25	5871.5
26	6999.0
27	7812.0
28	9007.0
29	10202.0
30	11647.0
31	13092.0
32	15002.5
33	16913.0
34	18355.5
35	19798.0
36	21071.0
37	22344.0
38	24203.0
39	26062.0
40	28381.5
41	30701.0
42	32669.0
43	34637.0
44	36153.0
45	37669.0
46	39254.5
47	40840.0
48	40039.0
49	39238.0
50	37455.5
51	35673.0
52	33566.0
53	31459.0
54	30027.5
55	28596.0
56	27526.5
57	26457.0
58	24688.5
59	22920.0
60	20798.5
61	18677.0
62	16585.5
63	14494.0
64	12342.5
65	10191.0
66	8803.5
67	7416.0
68	6636.0
69	5856.0
70	5191.0
71	4526.0
72	3787.0
73	3048.0
74	2323.5
75	1209.5
76	820.0
77	639.0
78	458.0
79	341.5
80	225.0
81	157.0
82	89.0
83	59.5
84	30.0
85	25.5
86	21.0
87	14.5
88	8.0
89	8.0
90	8.0
91	5.0
92	2.0
93	1.5
94	1.0
95	0.5
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	610651.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	52.89469221063495
#Duplication Level	Percentage of deduplicated	Percentage of total
1	71.49351890432094	37.816276774992666
2	11.787903357195036	12.470350397750837
3	5.881261350904099	9.332625268991263
4	3.456982227482647	7.314240436013193
5	2.205689900078756	5.833464418838597
6	1.515290324387591	4.809048919094089
7	1.0357645246838119	3.8350512015091383
8	0.7087975304757334	2.9993301771337655
9	0.5248959975650294	2.498779101041678
>10	1.3579676122831903	9.740584857829324
>50	0.020231780037150267	0.7464321493785387
>100	0.011064247851598654	0.8614732574079313
>500	0.0	0.0
>1k	3.161213671878894E-4	0.8297030868113551
>5k	3.161213671878894E-4	0.9126399532076532
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CGTTTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	5458	0.8938002230406565	No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	4962	0.8125754317932828	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.007369184689781889	0.0
2	0.0	0.0	0.0	0.030786816037311	0.0
3	0.0	0.0	0.0	0.04814533997324167	0.0
4	0.0	0.0	0.0	0.0658313832287182	0.0
5	0.0	0.0	0.0	0.11807071469628315	0.0
6	0.0	0.0	0.0	0.21092244178753494	0.0
7	0.0	0.0	0.0	0.27691758467602606	0.0
8	0.0	0.0	0.0	0.6558574373905881	0.0
9	0.0	0.0	0.0	0.8900337508658792	0.0
10	0.0	0.0	0.0	1.1987207095378538	0.0
11	0.0	0.0	0.0	1.3552749442807757	0.0
12	0.0	0.0	0.0	1.5124842176627893	0.0
13	0.0	0.0	0.0	1.5999318759815344	0.0
14	0.0	0.0	0.0	1.6546276023456934	0.0
15	0.0	0.0	0.0	1.693438641711878	0.0
16	0.0	0.0	0.0	1.7500994839933122	0.0
17	0.0	0.0	0.0	1.8152758285829385	0.0
18	0.0	0.0	0.0	1.9184444142398849	0.0
19	0.0	0.0	0.0	1.9695374280890394	0.0
20	0.0	0.0	0.0	2.0240693947934254	0.0
21	0.0	0.0	0.0	2.084824228569183	0.0
22	0.0	0.0	0.0	2.1516381697565383	0.0
23	0.0	0.0	0.0	2.223692420056628	0.0
24	0.0	0.0	0.0	2.287886206687617	0.0
25	0.0	0.0	0.0	2.34225441373223	0.0
26	0.0	0.0	0.0	2.394166225880249	0.0
27	0.0	0.0	0.0	2.4596700897894213	0.0
28	0.0	0.0	0.0	2.5218987605031353	0.0
29	0.0	0.0	0.0	2.5875663840720806	0.0
30	0.0	0.0	0.0	2.693518883945167	0.0
31	9.825579586375851E-4	0.0	0.0	2.763935537647527	0.0
32	9.825579586375851E-4	0.0	0.0	2.84679792549263	0.0
33	9.825579586375851E-4	0.0	0.0	2.927367678100912	0.0
34	9.825579586375851E-4	0.0	0.0	3.004989756833281	0.0
35	9.825579586375851E-4	0.0	0.0	3.0996428401820353	0.0
36	9.825579586375851E-4	0.0	0.0	3.1839790649650945	0.0
37	9.825579586375851E-4	0.0	0.0	3.26585889485156	0.0
38	9.825579586375851E-4	0.0	0.0	3.372466433363738	0.0
39	9.825579586375851E-4	0.0	0.0	3.516247414644371	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
AAATCGT	20	7.030687E-4	45.0	12
CGCATCG	25	3.8886505E-5	45.0	21
GTTACGG	25	3.8886505E-5	45.0	2
TTGACGA	25	3.8886505E-5	45.0	1
GCGATAC	20	7.030687E-4	45.0	9
CACGTTA	25	3.8886505E-5	45.0	1
GACGATA	25	3.8886505E-5	45.0	1
GCGCAAA	30	2.1638243E-6	44.999996	1
GCGTAAA	30	2.1638243E-6	44.999996	1
TGCACGG	60	0.0	44.999996	2
CGTTTTA	3365	0.0	44.39822	1
CGTTTTT	4370	0.0	44.227688	1
CGTTATT	680	0.0	42.683823	1
GTTTTAT	3630	0.0	42.210743	2
AGGCGAT	475	0.0	41.684208	7
ACGGGAT	140	0.0	40.178574	5
CGGTCTA	135	0.0	40.0	31
TAACGGG	85	0.0	39.705883	3
GTTATTT	755	0.0	39.63576	2
CTCACGA	160	0.0	39.375	24
>>END_MODULE