FastQCFastQC Report
Thu 26 May 2016
SRR1545716_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1545716_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences1085503
Sequences flagged as poor quality0
Sequence length50
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT41970.3866410318534357No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATA29820.27471135501237676No Hit
CGTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT18120.1669272217580237No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT17300.1593731201111374No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGAAATC207.856286E-444.00000414
CGCATCC207.856286E-444.00000422
TGCGGTC207.856286E-444.00000428
TATGCGT207.856286E-444.00000420
TCGATAT207.856286E-444.00000416
ATCCGGC207.856286E-444.00000444
GTATATC207.856286E-444.00000441
GTTATCA207.856286E-444.00000437
TGCCGTA207.856286E-444.00000425
TTGATCG254.4426757E-544.040
GGTCGTA351.4459874E-744.08
CACCTCG254.4426757E-544.035
TACGCTA302.5275858E-644.021
GTTAACG302.5275858E-644.01
CGAGGTA254.4426757E-544.05
TCGCTAG302.5275858E-644.02
ACTACGG351.4459874E-744.02
GTCGTTA254.4426757E-544.010
TCGTACA254.4426757E-544.034
ATGCGAA600.044.01
Produced by FastQC (version 0.11.5)