##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1545716_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	1085503
Sequences flagged as poor quality	0
Sequence length	50
%GC	45
>>END_MODULE
>>Per base sequence quality	fail
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.56158849860387	31.0	31.0	33.0	30.0	34.0
2	31.7455925962434	31.0	31.0	34.0	30.0	34.0
3	31.794238247153622	31.0	31.0	34.0	30.0	34.0
4	35.69063927045803	37.0	35.0	37.0	35.0	37.0
5	27.35992807021261	35.0	28.0	37.0	0.0	37.0
6	31.48548276697531	36.0	29.0	37.0	17.0	37.0
7	14.729854270324449	0.0	0.0	32.0	0.0	37.0
8	24.95002316898249	17.0	17.0	35.0	17.0	37.0
9	33.173187913805855	32.0	32.0	37.0	28.0	39.0
10	36.10260773116242	37.0	35.0	39.0	32.0	39.0
11	37.095058235675076	39.0	37.0	39.0	34.0	39.0
12	36.975640785884515	39.0	35.0	39.0	33.0	39.0
13	36.4184069505105	39.0	35.0	39.0	33.0	39.0
14	37.73557143554647	40.0	36.0	41.0	33.0	41.0
15	38.02549325059442	40.0	36.0	41.0	33.0	41.0
16	38.12388173961749	40.0	36.0	41.0	34.0	41.0
17	38.15410920098793	40.0	36.0	41.0	34.0	41.0
18	38.13880385406581	40.0	36.0	41.0	34.0	41.0
19	38.05529786651903	40.0	36.0	41.0	34.0	41.0
20	38.05015186508006	40.0	36.0	41.0	34.0	41.0
21	37.90373587175715	40.0	35.0	41.0	34.0	41.0
22	37.81708387724401	40.0	35.0	41.0	33.0	41.0
23	37.723208503339	39.0	35.0	41.0	33.0	41.0
24	37.58675747556663	39.0	35.0	41.0	33.0	41.0
25	37.4146566154124	39.0	35.0	41.0	33.0	41.0
26	37.157748988257055	39.0	35.0	40.0	33.0	41.0
27	36.953160884861674	39.0	35.0	40.0	33.0	41.0
28	37.00146291627015	39.0	35.0	40.0	33.0	41.0
29	36.9679365234366	38.0	35.0	40.0	33.0	41.0
30	36.79545611573621	38.0	35.0	40.0	32.0	41.0
31	36.593442855524124	38.0	35.0	40.0	32.0	41.0
32	36.33084293640828	38.0	35.0	40.0	31.0	41.0
33	36.116383833117	38.0	35.0	40.0	31.0	41.0
34	35.900288621956825	38.0	35.0	40.0	30.0	41.0
35	35.74794726500065	38.0	35.0	40.0	30.0	41.0
36	35.547816081576926	38.0	35.0	40.0	29.0	41.0
37	35.377709688503856	38.0	34.0	40.0	29.0	41.0
38	35.221125137378706	38.0	34.0	40.0	28.0	41.0
39	35.17501748037546	38.0	34.0	40.0	27.0	41.0
40	34.943959620563	37.0	34.0	40.0	27.0	41.0
41	34.792583714646575	37.0	34.0	40.0	26.0	40.0
42	34.78612864266612	37.0	34.0	40.0	26.0	41.0
43	34.65700417225931	37.0	34.0	40.0	26.0	41.0
44	34.42852576178969	37.0	34.0	40.0	25.0	40.0
45	34.20386585757939	36.0	33.0	39.0	24.0	40.0
46	34.17627496193009	36.0	33.0	39.0	24.0	40.0
47	34.021198467438595	36.0	33.0	39.0	24.0	40.0
48	33.995499782128654	36.0	33.0	39.0	24.0	40.0
49	33.883754351669225	36.0	33.0	39.0	24.0	40.0
50	33.92476575375655	36.0	33.0	39.0	24.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
12	1.0
13	3.0
14	6.0
15	13.0
16	47.0
17	150.0
18	368.0
19	771.0
20	1393.0
21	2376.0
22	3713.0
23	5990.0
24	9028.0
25	12924.0
26	16864.0
27	18739.0
28	19053.0
29	21304.0
30	26251.0
31	35361.0
32	50647.0
33	97906.0
34	95001.0
35	114487.0
36	141964.0
37	263210.0
38	120256.0
39	27677.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	33.094335068627174	22.971378245845475	28.15994059896656	15.774346086560792
2	33.01907042173076	24.34078947732065	30.565461357545765	12.074678743402828
3	29.6336352824451	24.26008956216611	33.64974578605494	12.456529369333849
4	26.857502927214387	26.433552003080603	31.99521327900522	14.713731790699796
5	17.14467854994413	48.074118634402666	23.88634577702687	10.894857038626332
6	20.223067094241102	40.68049558591731	28.6358490027204	10.460588317121188
7	38.15917597648279	56.715181809723234	4.0828076937604045	1.0428345200335696
8	86.6404791142908	3.2364719397366937	8.28731012258833	1.8357388233841823
9	82.72846781630267	4.743607341481323	9.57786390272528	2.9500609394907245
10	54.03301510912453	20.580873567369228	14.309126736637301	11.076984586868944
11	48.55748901661258	18.082216262875367	19.851442142490626	13.508852578021433
12	43.022174973261244	19.46305077001169	24.140237290914904	13.374536965812162
13	21.427946306919466	39.78883522201228	23.763269194097113	15.019949276971136
14	15.187337114683238	42.22300629293517	27.582512438933836	15.007144153447758
15	13.979325713517143	21.71619977098175	49.41312921290867	14.891345302592438
16	15.456152585483412	15.240584318974706	49.114742197856664	20.18852089768522
17	15.84187238542869	16.215063431423037	30.354038634623766	37.58902554852451
18	21.304040615272367	21.782620591559855	35.57060643775282	21.342732355414956
19	30.32124277869338	23.33010595088176	25.748339709793523	20.600311560631337
20	32.67545091998825	21.24508177315033	24.73056269766182	21.348904609199607
21	22.65309262157728	26.660267175678005	28.066159190716196	22.620481012028527
22	22.23319511783938	23.47381812855423	25.601034727679238	28.691952025927154
23	20.303582763014013	29.430043030742432	24.948526167131735	25.317848039111823
24	21.16640856819373	22.81338697359657	38.782297239160094	17.237907219049603
25	18.451538134855454	22.54005746644643	36.59105502241818	22.417349376279937
26	17.1333473974738	32.95983521003627	28.327512683060295	21.579304709429636
27	17.905892475654145	32.046802265862	30.460072427252623	19.587232831231237
28	16.186873735033437	26.907525819827306	40.022643880302496	16.882956564836764
29	16.269323990813476	23.386301097279325	38.6096583795715	21.734716532335703
30	19.176087030620828	28.526498775222176	32.679412217193324	19.618001976963676
31	30.210050087378846	24.785191749815525	24.836412243909045	20.168345918896584
32	30.515346341742035	25.371095243403293	26.633090834387374	17.480467580467305
33	28.885226480258464	26.091867088345218	24.930838514495125	20.092067916901197
34	20.314545422721082	25.83843619041127	28.669934583322203	25.177083803545454
35	20.638819054392297	24.3128761505035	31.819718600501336	23.228586194602872
36	30.9503520487737	23.520616709488596	26.26349259283484	19.265538648902858
37	21.27566667250114	31.25537193356444	28.54823984825468	18.920721545679744
38	21.524952026848382	31.687245452108375	23.873080037549414	22.914722483493826
39	21.8098890560413	30.03520027121067	26.625720979122118	21.529189693625906
40	23.626558378926635	24.69233157347331	26.84617177474406	24.834938272855993
41	18.124592930650582	23.61983338599709	27.35552089676399	30.90005278658834
42	22.265069741861605	25.810983479548188	23.091599009859944	28.832347768730255
43	22.64986830989873	25.74502327492416	24.657324760963352	26.947783654213758
44	20.194140412324977	29.444414248509677	27.882557671420532	22.478887667744814
45	18.146518250064716	36.49626025906883	23.103390778284354	22.253830712582094
46	22.62536354114176	31.215298345559617	26.308356586762084	19.850981526536547
47	22.9092872152357	26.707710618948084	26.40683627774405	23.976165888072167
48	23.236600912203837	22.991921717397375	30.485774797490194	23.285702572908594
49	21.372396022857608	23.20896395495913	32.53606853228411	22.882571489899153
50	19.81542197488169	31.11930598072967	27.19946421152222	21.86580783286642
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	886.0
1	1370.5
2	1855.0
3	7441.5
4	13028.0
5	9612.5
6	6197.0
7	6448.0
8	6699.0
9	7012.5
10	7326.0
11	7318.0
12	7310.0
13	7067.5
14	6825.0
15	6184.5
16	5544.0
17	5178.5
18	4813.0
19	4454.5
20	4096.0
21	4291.5
22	4487.0
23	4755.0
24	5023.0
25	5947.5
26	6872.0
27	7813.0
28	8754.0
29	10908.5
30	13063.0
31	14975.5
32	16888.0
33	18474.0
34	20060.0
35	21025.5
36	21991.0
37	27383.5
38	32776.0
39	48054.0
40	63332.0
41	80838.5
42	98345.0
43	104004.0
44	109663.0
45	105299.5
46	100936.0
47	94541.5
48	88147.0
49	84240.0
50	80333.0
51	75084.0
52	69835.0
53	62883.5
54	55932.0
55	51005.5
56	46079.0
57	44992.5
58	43906.0
59	41312.0
60	38718.0
61	34731.0
62	30744.0
63	26485.0
64	22226.0
65	18368.5
66	14511.0
67	12083.0
68	9655.0
69	8531.0
70	7407.0
71	6494.5
72	5582.0
73	4192.5
74	2803.0
75	2107.5
76	1412.0
77	1149.5
78	887.0
79	602.0
80	317.0
81	217.5
82	118.0
83	94.5
84	71.0
85	51.5
86	32.0
87	19.5
88	7.0
89	5.0
90	3.0
91	3.0
92	3.0
93	4.0
94	5.0
95	3.0
96	1.0
97	0.5
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
50	1085503.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	20.546329486893118
#Duplication Level	Percentage of deduplicated	Percentage of total
1	72.12030372333787	14.818075229945043
2	6.105674062579356	2.5089838205866544
3	2.7764489038179154	1.7113750194409834
4	1.8045284934843038	1.4830574798246146
5	1.413115255671467	1.4517165822990583
6	1.17070109097958	1.4432166207559014
7	0.9557776548078326	1.3746405828324206
8	0.877667602190843	1.4426278187667598
9	0.7994576681277822	1.4783328594158998
>10	10.425768728721854	47.90524197861028
>50	1.4087375953042978	19.079498723508
>100	0.1394555672939648	4.20791164035452
>500	4.727307365897112E-4	0.0540036209873996
>1k	0.0018909229463588448	1.041318022672502
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CGTTTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	4197	0.3866410318534357	No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATA	2982	0.27471135501237676	No Hit
CGTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1812	0.1669272217580237	No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1730	0.1593731201111374	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.011607522042776482	0.0
2	0.0	0.0	0.0	0.040442080768086315	0.0
3	0.0	0.0	0.0	0.052786588337388286	0.0
4	0.0	0.0	0.0	0.07148759607297263	0.0
5	0.0	0.0	0.0	0.11321940151247854	0.0
6	0.0	0.0	0.0	0.18221967143342763	0.0
7	0.0	0.0	0.0	0.22616243345251003	0.0
8	0.0	0.0	0.0	0.47425018631915344	0.0
9	0.0	0.0	0.0	0.589496298029577	0.0
10	0.0	0.0	0.0	0.8063542892097028	0.0
11	0.0	0.0	0.0	0.9855338953462127	0.0
12	0.0	0.0	0.0	1.1551326896378913	0.0
13	0.0	0.0	0.0	1.2244093291312874	0.0
14	0.0	0.0	0.0	1.2511250544678365	0.0
15	0.0	0.0	0.0	1.2917513816175543	0.0
16	0.0	0.0	0.0	1.3896783334546288	0.0
17	0.0	0.0	0.0	1.4996734232885585	0.0
18	0.0	0.0	0.0	1.6761814568914135	0.0
19	0.0	0.0	0.0	1.7399307049358685	0.0
20	0.0	0.0	0.0	1.803219337026245	0.0
21	0.0	0.0	0.0	1.888709658103202	0.0
22	0.0	0.0	0.0	1.9714362834556882	0.0
23	0.0	0.0	0.0	2.076640967367202	0.0
24	0.0	0.0	0.0	2.1508922591646455	0.0
25	0.0	0.0	0.0	2.209482608523422	0.0
26	0.0	0.0	0.0	2.269362682553618	0.0
27	0.0	0.0	0.0	2.325465705760371	0.0
28	0.0	0.0	0.0	2.38506941021812	0.0
29	0.0	0.0	0.0	2.452042969941124	0.0
30	0.0	0.0	0.0	2.540389109933367	0.0
31	0.0	0.0	0.0	2.6210890250879086	0.0
32	0.0	0.0	0.0	2.697919766228191	0.0
33	0.0	0.0	0.0	2.772447427598081	0.0
34	0.0	0.0	0.0	2.8434744077169753	0.0
35	0.0	0.0	0.0	2.948494845246858	0.0
36	0.0	0.0	0.0	3.0339851663238147	0.0
37	0.0	0.0	0.0	3.1183700091109836	0.0
38	0.0	0.0	0.0	3.2008202648910227	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGAAATC	20	7.856286E-4	44.000004	14
CGCATCC	20	7.856286E-4	44.000004	22
TGCGGTC	20	7.856286E-4	44.000004	28
TATGCGT	20	7.856286E-4	44.000004	20
TCGATAT	20	7.856286E-4	44.000004	16
ATCCGGC	20	7.856286E-4	44.000004	44
GTATATC	20	7.856286E-4	44.000004	41
GTTATCA	20	7.856286E-4	44.000004	37
TGCCGTA	20	7.856286E-4	44.000004	25
TTGATCG	25	4.4426757E-5	44.0	40
GGTCGTA	35	1.4459874E-7	44.0	8
CACCTCG	25	4.4426757E-5	44.0	35
TACGCTA	30	2.5275858E-6	44.0	21
GTTAACG	30	2.5275858E-6	44.0	1
CGAGGTA	25	4.4426757E-5	44.0	5
TCGCTAG	30	2.5275858E-6	44.0	2
ACTACGG	35	1.4459874E-7	44.0	2
GTCGTTA	25	4.4426757E-5	44.0	10
TCGTACA	25	4.4426757E-5	44.0	34
ATGCGAA	60	0.0	44.0	1
>>END_MODULE