FastQCFastQC Report
Thu 26 May 2016
SRR1545715_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1545715_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences409912
Sequences flagged as poor quality0
Sequence length51
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT11060.2698140088604383No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATAC10940.2668865512597826No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT9700.2366361560530065No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTAGGAT1350.045.0000045
ATAGGCG453.8380676E-1045.05
TAGACAC406.7957444E-945.043
GTCTCGA207.02797E-445.035
TACGGGT253.886399E-545.04
GTTACGG253.886399E-545.02
CTACGGG253.886399E-545.03
GTATATC207.02797E-445.041
TTACGGG551.8189894E-1245.03
ATGCGAA253.886399E-545.01
GCTACGA253.886399E-545.010
AGTACGG502.1827873E-1145.02
TAGTACG207.02797E-445.01
CGAAGGT207.02797E-445.04
CGACGGT600.044.99999628
ACTAGGT302.1620726E-644.9999964
GACGGTC600.044.99999629
ACGCTCC302.1620726E-644.99999614
GCGATAT302.1620726E-644.9999969
ACGACGG600.044.99999627
Produced by FastQC (version 0.11.5)