##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1545715_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	409912
Sequences flagged as poor quality	0
Sequence length	51
%GC	45
>>END_MODULE
>>Per base sequence quality	fail
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.06388200394231	31.0	31.0	33.0	30.0	33.0
2	31.217349089560685	31.0	31.0	33.0	30.0	34.0
3	31.151222701457876	31.0	31.0	34.0	28.0	34.0
4	35.130942738929335	35.0	35.0	37.0	33.0	37.0
5	32.92982396221628	35.0	35.0	37.0	28.0	37.0
6	34.09178311442456	35.0	35.0	37.0	29.0	37.0
7	14.71486075060013	0.0	0.0	35.0	0.0	37.0
8	24.60987480239661	17.0	17.0	35.0	17.0	37.0
9	32.63107203497336	32.0	32.0	37.0	27.0	39.0
10	35.547934678662735	37.0	35.0	37.0	32.0	39.0
11	36.551955053767635	37.0	35.0	39.0	33.0	39.0
12	36.330932004918125	37.0	35.0	39.0	32.0	39.0
13	35.87827387341673	37.0	35.0	39.0	31.0	39.0
14	36.93359062432913	39.0	35.0	40.0	31.0	41.0
15	37.32582359140498	39.0	35.0	41.0	32.0	41.0
16	37.422019848162535	39.0	35.0	41.0	32.0	41.0
17	37.39884170260934	39.0	35.0	41.0	32.0	41.0
18	37.31321600733816	39.0	35.0	41.0	32.0	41.0
19	37.223147895157986	39.0	35.0	40.0	32.0	41.0
20	37.31498955873456	39.0	35.0	41.0	32.0	41.0
21	37.143828431468215	39.0	35.0	40.0	32.0	41.0
22	37.205183063681964	39.0	35.0	41.0	32.0	41.0
23	37.19102880618279	39.0	35.0	41.0	32.0	41.0
24	37.029811276566676	39.0	35.0	40.0	32.0	41.0
25	36.79459005835399	38.0	35.0	40.0	32.0	41.0
26	36.65339877827436	38.0	35.0	40.0	31.0	41.0
27	36.545470735182185	38.0	35.0	40.0	31.0	41.0
28	36.48285729620016	38.0	35.0	40.0	31.0	41.0
29	36.50562559768926	38.0	35.0	40.0	31.0	41.0
30	36.271014266476705	38.0	35.0	40.0	30.0	41.0
31	36.123897324303755	38.0	35.0	40.0	30.0	41.0
32	35.99027108257382	38.0	35.0	40.0	30.0	41.0
33	35.78916450360077	38.0	34.0	40.0	29.0	41.0
34	35.63377261460997	38.0	34.0	40.0	29.0	41.0
35	35.448415757528444	38.0	34.0	40.0	27.0	41.0
36	35.25526698413318	38.0	34.0	40.0	26.0	41.0
37	35.09116590878042	38.0	34.0	40.0	26.0	41.0
38	35.04044526630106	38.0	34.0	40.0	26.0	41.0
39	34.940953180194775	38.0	34.0	40.0	25.0	41.0
40	34.76336384394699	37.0	34.0	40.0	24.0	41.0
41	34.65102509806983	37.0	34.0	40.0	24.0	41.0
42	34.605554362887645	37.0	33.0	40.0	24.0	41.0
43	34.513524854115026	37.0	33.0	40.0	23.0	41.0
44	34.28053826187084	37.0	33.0	40.0	23.0	41.0
45	34.18208786276079	37.0	33.0	40.0	23.0	41.0
46	34.17831388200394	36.0	33.0	40.0	23.0	41.0
47	34.12889351860887	36.0	33.0	40.0	23.0	41.0
48	34.11088965436484	36.0	33.0	40.0	23.0	41.0
49	34.023910009953354	36.0	33.0	40.0	23.0	41.0
50	33.87326548137161	36.0	33.0	40.0	23.0	41.0
51	33.66810681316966	36.0	33.0	40.0	23.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
12	3.0
13	1.0
14	5.0
15	14.0
16	50.0
17	106.0
18	228.0
19	435.0
20	806.0
21	1299.0
22	2028.0
23	3096.0
24	4277.0
25	5927.0
26	7071.0
27	7613.0
28	8088.0
29	9793.0
30	11890.0
31	15390.0
32	20737.0
33	31853.0
34	41330.0
35	41989.0
36	47279.0
37	71804.0
38	65140.0
39	11660.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	32.67286637131872	23.609457639688518	28.370479517554987	15.34719647143777
2	32.49551123167899	24.24764339663147	31.064472374558445	12.192372997131091
3	30.212582212767618	23.965387692968246	33.363746365073474	12.458283729190656
4	27.539569468568864	27.146802240480884	30.449218368820624	14.864409922129628
5	20.902535178282168	37.076494467105135	28.64663635121685	13.374334003395852
6	20.195066258123695	41.40205702687406	28.089199633091983	10.313677081910264
7	36.35365639454322	58.61745935713031	4.269452955756357	0.7594312925701125
8	87.25824079314584	3.4390308163703427	7.506733152481508	1.7959952380023028
9	83.23932941704561	4.8720213118913325	8.794570541969984	3.0940787290930736
10	54.428023575791876	20.968646929096977	13.642196373855853	10.961133121255294
11	49.36010655945666	18.206590682878275	19.236811803509045	13.196490954156015
12	43.703282655789536	19.78424637483167	23.427711313647805	13.084759655730988
13	21.55072308202736	40.849255449950235	22.864419680321628	14.735601787700775
14	14.995657604559026	43.3651613029138	26.886990378422688	14.75219071410449
15	13.728556373075197	22.130115732157147	49.27106305743672	14.870264837330941
16	15.342561330236734	15.571390932687992	48.97563379457054	20.11041394250473
17	15.656287203107007	16.47817092449111	29.673686059446908	38.19185581295498
18	21.195036983547688	22.150607935361734	35.51493979195535	21.13941528913523
19	31.06666796775893	23.307197642420814	25.2651788676594	20.360955522160854
20	33.35154862507075	21.3558031967837	24.18250746501688	21.110140713128672
21	22.618757196666603	27.485167548156674	27.607876812584166	22.28819844259256
22	22.191104432170807	23.70142859930912	24.82728000156131	29.28018696695876
23	20.33143699135424	30.070600519135816	24.336198989051308	25.261763500458635
24	20.668826479829818	23.097884423973927	39.15620913757099	17.077079958625266
25	18.13999102246336	22.999814594351957	36.69763266262027	22.162561720564415
26	16.920948886590292	33.887029411190696	27.76425183941919	21.42776986279982
27	17.655740744354887	33.1861472706337	29.67246628544663	19.485645699564785
28	15.954399968773785	27.537129920568315	39.80683658931673	16.701633521341165
29	16.048322566794823	23.819502722535567	38.52656179862995	21.605612912039657
30	19.0901949686762	29.57195690782412	31.963201857959756	19.374646265539923
31	30.9834793809403	25.092946778820817	24.15030543140967	19.77326840882921
32	31.374782880227954	25.29201389566541	26.233679423876342	17.099523800230294
33	29.75955814906614	26.17878959386405	24.172017408614533	19.88963484845528
34	20.37217744296337	25.875065867796014	28.130915903901325	25.621840785339295
35	20.62125529381916	24.235689611428796	32.02419055797342	23.118864536778624
36	31.59312242627686	23.78339741212748	25.756503834969458	18.866976326626204
37	21.085013368723043	32.363531684849434	27.906721442651104	18.64473350377642
38	21.273590429165285	32.78874490134468	23.349889732430377	22.587774937059663
39	21.70539042526201	30.96323113253576	26.074620894240713	21.256757547961513
40	24.169333905813932	24.687493901129997	26.441041003922795	24.70213118913328
41	18.010451023634342	23.54700521087453	27.05361150686001	31.38893225863112
42	21.94397821971545	25.699418411756668	22.781719003103102	29.574884365424776
43	22.294785222194033	25.560608130525576	24.534046331895627	27.610560315384763
44	19.874753605651946	29.5790315970257	28.413659517164657	22.132555280157693
45	18.113643904057454	37.39095220437557	22.63510216827026	21.860301723296708
46	22.345771775405453	32.151778918401995	25.7928531001776	19.709596206014947
47	22.644128495872284	26.93700111243389	26.008265188625852	24.410605203067977
48	22.952975272741465	23.175462050391303	30.970061866937293	22.901500809929935
49	21.280421163566814	23.588477526883818	32.78606139854408	22.345039911005287
50	19.56688264798298	32.30083530123539	26.773307441597222	21.35897460918441
51	19.523458693573254	33.56232557231797	24.340834130252347	22.573381603856436
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	320.0
1	511.0
2	702.0
3	2327.0
4	3952.0
5	3039.0
6	2126.0
7	2200.0
8	2274.0
9	2293.5
10	2313.0
11	2334.5
12	2356.0
13	2308.5
14	2261.0
15	2103.0
16	1945.0
17	1796.5
18	1648.0
19	1581.5
20	1515.0
21	1557.5
22	1600.0
23	1682.0
24	1764.0
25	1999.0
26	2685.0
27	3136.0
28	3939.0
29	4742.0
30	5331.0
31	5920.0
32	6812.5
33	7705.0
34	8126.5
35	8548.0
36	9957.5
37	11367.0
38	16262.0
39	21157.0
40	27320.0
41	33483.0
42	37015.0
43	40547.0
44	39281.0
45	38015.0
46	36351.5
47	34688.0
48	32006.5
49	29325.0
50	28202.0
51	27079.0
52	25122.5
53	23166.0
54	21172.5
55	19179.0
56	18198.5
57	17218.0
58	16182.0
59	15146.0
60	13895.5
61	12645.0
62	11034.0
63	9423.0
64	7973.5
65	6524.0
66	5490.5
67	4457.0
68	3829.5
69	3202.0
70	2875.0
71	2548.0
72	2208.0
73	1868.0
74	1401.5
75	669.0
76	403.0
77	316.0
78	229.0
79	189.5
80	150.0
81	98.0
82	46.0
83	36.0
84	26.0
85	15.0
86	4.0
87	6.5
88	9.0
89	7.5
90	6.0
91	4.0
92	2.0
93	2.5
94	3.0
95	2.0
96	1.0
97	0.5
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	409912.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	29.401145811120386
#Duplication Level	Percentage of deduplicated	Percentage of total
1	68.22642903955976	20.05935188364155
2	7.543042614801916	4.4354819155457195
3	4.072022315434029	3.591663655266358
4	2.889504558570316	3.398189793936917
5	2.1790422164295093	3.2033168966915473
6	1.820233479741203	3.2110169968892497
7	1.4277695929177807	2.9384643390241774
8	1.274331535568581	2.9973445831168637
9	1.093577006820595	2.8937175329898817
>10	9.317349046333426	49.21523827921561
>50	0.13680035968664433	2.5517097023259807
>100	0.017410954869209277	0.7317761297652459
>500	8.290930890099656E-4	0.23644998196944988
>1k	0.0016581861780199311	0.5362783096214327
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CGTTTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1106	0.2698140088604383	No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATAC	1094	0.2668865512597826	No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	970	0.2366361560530065	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.010490056402349773	0.0
2	0.0	0.0	0.0	0.03927672280879799	0.0
3	0.0	0.0	0.0	0.050742598411366344	0.0
4	0.0	0.0	0.0	0.06903920841546478	0.0
5	0.0	0.0	0.0	0.1068522024239349	0.0
6	0.0	0.0	0.0	0.1478366088331154	0.0
7	0.0	0.0	0.0	0.1761353656394543	0.0
8	0.0	0.0	0.0	0.3844727648861219	0.0
9	0.0	0.0	0.0	0.4796151369074338	0.0
10	0.0	0.0	0.0	0.6552625929467788	0.0
11	0.0	0.0	0.0	0.8050508401803314	0.0
12	0.0	0.0	0.0	0.9428853022112063	0.0
13	0.0	0.0	0.0	0.9943597650227366	0.0
14	0.0	0.0	0.0	1.0197310642284199	0.0
15	0.0	0.0	0.0	1.0585198774371085	0.0
16	0.0	0.0	0.0	1.1363414586545404	0.0
17	0.0	0.0	0.0	1.2229454126739399	0.0
18	0.0	0.0	0.0	1.3851753547102792	0.0
19	0.0	0.0	0.0	1.43982122992252	0.0
20	0.0	0.0	0.0	1.5008099299361815	0.0
21	0.0	0.0	0.0	1.5722886863521928	0.0
22	0.0	0.0	0.0	1.6466949003688596	0.0
23	0.0	0.0	0.0	1.7323230351880403	0.0
24	0.0	0.0	0.0	1.7969710572025215	0.0
25	0.0	0.0	0.0	1.8482015652139971	0.0
26	2.4395480005464588E-4	0.0	0.0	1.8996760280255274	0.0
27	2.4395480005464588E-4	0.0	0.0	1.9452955756357462	0.0
28	2.4395480005464588E-4	0.0	0.0	1.9977458576474951	0.0
29	2.4395480005464588E-4	0.0	0.0	2.058246648061047	0.0
30	2.4395480005464588E-4	0.0	0.0	2.1346045004781513	0.0
31	2.4395480005464588E-4	0.0	0.0	2.200960206093015	0.0
32	2.4395480005464588E-4	0.0	0.0	2.2646324089072776	0.0
33	2.4395480005464588E-4	0.0	0.0	2.333915572122797	0.0
34	2.4395480005464588E-4	0.0	0.0	2.408321786139464	0.0
35	2.4395480005464588E-4	0.0	0.0	2.513710259763071	0.0
36	2.4395480005464588E-4	0.0	0.0	2.5834813325786996	0.0
37	2.4395480005464588E-4	0.0	0.0	2.657155682195203	0.0
38	2.4395480005464588E-4	0.0	0.0	2.7283904838111597	0.0
39	2.4395480005464588E-4	0.0	0.0	2.8006011046273347	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TTAGGAT	135	0.0	45.000004	5
ATAGGCG	45	3.8380676E-10	45.0	5
TAGACAC	40	6.7957444E-9	45.0	43
GTCTCGA	20	7.02797E-4	45.0	35
TACGGGT	25	3.886399E-5	45.0	4
GTTACGG	25	3.886399E-5	45.0	2
CTACGGG	25	3.886399E-5	45.0	3
GTATATC	20	7.02797E-4	45.0	41
TTACGGG	55	1.8189894E-12	45.0	3
ATGCGAA	25	3.886399E-5	45.0	1
GCTACGA	25	3.886399E-5	45.0	10
AGTACGG	50	2.1827873E-11	45.0	2
TAGTACG	20	7.02797E-4	45.0	1
CGAAGGT	20	7.02797E-4	45.0	4
CGACGGT	60	0.0	44.999996	28
ACTAGGT	30	2.1620726E-6	44.999996	4
GACGGTC	60	0.0	44.999996	29
ACGCTCC	30	2.1620726E-6	44.999996	14
GCGATAT	30	2.1620726E-6	44.999996	9
ACGACGG	60	0.0	44.999996	27
>>END_MODULE