FastQCFastQC Report
Thu 26 May 2016
SRR1545714_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1545714_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences890969
Sequences flagged as poor quality0
Sequence length50
%GC44

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT36640.41123765248847044No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATA25220.2830625981375334No Hit
CGTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT15630.17542697894090592No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT15570.17475355483748595No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GACCGTT302.5270729E-644.0000049
TCCCTAG207.8555266E-444.0000042
TAGCCGT207.8555266E-444.00000444
TACGCTT207.8555266E-444.00000423
TACGCAA302.5270729E-644.0000041
GCCGAAC207.8555266E-444.00000431
AAGTTCG207.8555266E-444.00000431
TACGAGT207.8555266E-444.00000427
TGTTACG207.8555266E-444.0000041
TCATCGG207.8555266E-444.00000444
TATAGCG207.8555266E-444.0000041
CGTAAAT600.044.00000416
TTACGCT207.8555266E-444.00000422
CGAGACA600.044.00000422
GTCCAAT207.8555266E-444.0000049
GCGTAAA302.5270729E-644.0000041
TCGCCGA254.4420318E-544.043
TTTACGG900.044.02
GCGAATA254.4420318E-544.01
ACGTTAG502.7284841E-1144.02
Produced by FastQC (version 0.11.5)