##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1545714_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	890969
Sequences flagged as poor quality	0
Sequence length	50
%GC	44
>>END_MODULE
>>Per base sequence quality	fail
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.539995218688865	31.0	31.0	33.0	30.0	34.0
2	31.722680587091133	31.0	31.0	34.0	30.0	34.0
3	31.760432742328856	31.0	31.0	34.0	30.0	34.0
4	35.656852258608325	37.0	35.0	37.0	33.0	37.0
5	27.315788764816734	35.0	28.0	37.0	0.0	37.0
6	31.447472358746488	35.0	28.0	37.0	17.0	37.0
7	14.791515754195713	0.0	0.0	32.0	0.0	37.0
8	24.990505842515283	17.0	17.0	35.0	17.0	37.0
9	33.203958835829305	32.0	32.0	37.0	28.0	39.0
10	36.08370324893459	37.0	35.0	39.0	32.0	39.0
11	37.0828199409856	39.0	37.0	39.0	34.0	39.0
12	36.91231120274667	39.0	35.0	39.0	33.0	39.0
13	36.36216074857823	39.0	35.0	39.0	32.0	39.0
14	37.675197453558994	40.0	36.0	41.0	33.0	41.0
15	38.00635375641577	40.0	36.0	41.0	33.0	41.0
16	38.08792449568953	40.0	36.0	41.0	33.0	41.0
17	38.11156617121359	40.0	36.0	41.0	34.0	41.0
18	38.086018705476846	40.0	36.0	41.0	34.0	41.0
19	37.992361125920205	40.0	36.0	41.0	34.0	41.0
20	37.998916909567	40.0	36.0	41.0	34.0	41.0
21	37.836123366806255	40.0	35.0	41.0	34.0	41.0
22	37.733168045128394	39.0	35.0	41.0	33.0	41.0
23	37.639455469269976	39.0	35.0	41.0	33.0	41.0
24	37.49830016532562	39.0	35.0	41.0	33.0	41.0
25	37.32043202400981	39.0	35.0	41.0	33.0	41.0
26	37.05939039405411	39.0	35.0	40.0	33.0	41.0
27	36.83006030512846	38.0	35.0	40.0	32.0	41.0
28	36.87326270610986	38.0	35.0	40.0	32.0	41.0
29	36.86189194012362	38.0	35.0	40.0	32.0	41.0
30	36.66141695165601	38.0	35.0	40.0	32.0	41.0
31	36.41617946303407	38.0	35.0	40.0	31.0	41.0
32	36.14073778100024	38.0	35.0	40.0	31.0	41.0
33	35.92557653521054	38.0	35.0	40.0	30.0	41.0
34	35.67632543893222	38.0	35.0	40.0	30.0	41.0
35	35.49030774359153	38.0	35.0	40.0	29.0	41.0
36	35.26923495654731	38.0	34.0	40.0	27.0	41.0
37	35.08168073187731	38.0	34.0	40.0	27.0	41.0
38	34.92550694805319	38.0	34.0	40.0	26.0	41.0
39	34.890943455945155	38.0	34.0	40.0	26.0	41.0
40	34.623930798939135	37.0	34.0	40.0	24.0	41.0
41	34.46585795914336	37.0	34.0	40.0	24.0	40.0
42	34.454272819817525	37.0	34.0	40.0	24.0	41.0
43	34.316578915764744	37.0	33.0	40.0	23.0	41.0
44	34.090540748331314	36.0	33.0	40.0	23.0	40.0
45	33.84755025146779	36.0	33.0	39.0	23.0	40.0
46	33.814783679342376	36.0	33.0	39.0	23.0	40.0
47	33.68241880469466	36.0	33.0	39.0	23.0	40.0
48	33.6700412696738	36.0	33.0	39.0	23.0	40.0
49	33.57056642823712	36.0	33.0	39.0	23.0	40.0
50	33.59002052821142	36.0	33.0	39.0	23.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
12	3.0
13	3.0
14	8.0
15	24.0
16	51.0
17	147.0
18	361.0
19	750.0
20	1423.0
21	2375.0
22	3904.0
23	5823.0
24	8605.0
25	12377.0
26	15566.0
27	16649.0
28	16986.0
29	18476.0
30	22488.0
31	29951.0
32	41174.0
33	79404.0
34	77585.0
35	92784.0
36	112647.0
37	212072.0
38	97384.0
39	21949.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	31.101867741750834	22.16743792432733	30.38512002101083	16.345574312910998
2	33.363001406334	25.420413055897566	29.240074570495718	11.976510967272711
3	28.526020546169395	23.510133349196213	35.3082991664132	12.655546938221196
4	26.10764235343766	27.066598276707722	32.69047520171858	14.135284168136042
5	17.16277446241115	47.10511813542334	25.001655500920904	10.730451901244601
6	19.674983080219402	40.15717718573823	29.71820568392391	10.449634050118467
7	38.02960596833335	56.501965837195236	4.551673515015674	0.9167546794557385
8	86.65946851125011	3.138717508689977	8.566628019605622	1.635185960454292
9	83.22601571996331	4.354248015363049	9.986430504316088	2.4333057603575434
10	53.523298790418075	20.1758983758133	15.45541988554035	10.845382948228277
11	48.83649150531612	17.668067014677277	20.466256401737883	13.029185078268716
12	42.0075221472352	20.423942920572994	24.48457802684493	13.083956905346875
13	20.365018311523745	41.5548689123864	24.851032976455972	13.22907979963388
14	13.972652247160115	42.48812248237593	29.264093363517695	14.275131906946257
15	12.695615672374686	20.880412225341175	51.28023533927667	15.143736763007468
16	14.17411829143326	15.536679727353029	50.03383956119686	20.25536242001686
17	14.905569105097932	15.976537904236848	30.752921818828717	38.3649711718365
18	20.590054199416592	21.020933388254807	36.86289870915823	21.526113703170367
19	28.904372654940858	23.13043439221791	26.71260167300995	21.252591279831286
20	30.99647686956561	21.589640043592986	25.506835815836464	21.907047271004938
21	21.726008424535532	27.12978790507863	29.165548969717243	21.9786547006686
22	21.91591402169997	23.288464581820467	26.16432221547551	28.631299181004056
23	18.795491201152903	29.18900657598637	25.64410209558357	26.371400127277155
24	19.166435644786745	22.19415041376299	41.379105221393786	17.26030872005648
25	17.57580791250874	23.065448966237884	38.03544231056299	21.323300810690384
26	16.17082075807351	33.3674909003568	29.065882202411082	21.3958061391586
27	17.0814023832479	32.38047563944424	31.206809664533786	19.331312312774067
28	15.02442845935156	26.757496613237947	40.79614442253322	17.421930504877274
29	14.820942142768153	22.66027213068019	40.098813763441825	22.419971963109827
30	18.950603219640637	28.490441306038704	33.464800683300986	19.094154791019665
31	28.850610964017825	25.610767602464286	26.05556422277318	19.483057210744708
32	29.16162066244729	25.838272712069667	27.646865379154605	17.353241246328434
33	27.8028752964469	26.12324334516689	25.907186445319645	20.16669491306656
34	19.143763699971604	26.771301807358057	29.362749994668725	24.722184498001614
35	19.929537391312156	24.374136473884054	32.340182430589614	23.356143704214176
36	30.53091633940126	23.518438913138393	27.060874171828647	18.8897705756317
37	19.560164270586295	31.6960522756684	28.771708106567118	19.972075347178183
38	21.768097430999283	31.748579355735163	24.04730130902422	22.436021904241336
39	20.67131404122927	29.808893463184464	27.16390805965191	22.35588443593436
40	23.554803814723073	24.554389658899467	26.310567483268215	25.580239043109245
41	17.69365713060724	23.322360261692605	28.193349039079923	30.790633568620233
42	22.78956955853683	25.438258794638198	24.03304716550183	27.739124481323145
43	22.417278266696147	26.352880964433105	25.40357745331207	25.826263315558677
44	19.899570018709966	29.98342254332081	28.116803165991183	22.00020427197804
45	17.560543632831223	36.41394930687824	23.759636979513317	22.265870080777223
46	22.45858161170591	30.381416188442024	27.55022902031384	19.609773179538234
47	22.767122088422827	26.497442671967264	27.14954167877895	23.58589356083096
48	23.097099899098623	23.414170414458866	30.01888954610093	23.46984014034158
49	21.06560385378167	23.50990887449507	32.832343212839056	22.5921440588842
50	19.840869884361858	31.880570480005478	27.145052184756146	21.133507450876518
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	848.0
1	1132.5
2	1417.0
3	6349.0
4	11281.0
5	8430.5
6	5580.0
7	5857.5
8	6135.0
9	6568.5
10	7002.0
11	6981.5
12	6961.0
13	6567.5
14	6174.0
15	5797.0
16	5420.0
17	5001.5
18	4583.0
19	4405.5
20	4228.0
21	4376.0
22	4524.0
23	4534.5
24	4545.0
25	5208.5
26	5872.0
27	6970.5
28	8069.0
29	9892.5
30	11716.0
31	13649.0
32	15582.0
33	16803.0
34	18024.0
35	19148.5
36	20273.0
37	24656.0
38	29039.0
39	41656.0
40	54273.0
41	67181.5
42	80090.0
43	84623.5
44	89157.0
45	87096.5
46	85036.0
47	80099.5
48	75163.0
49	72311.0
50	69459.0
51	61769.5
52	54080.0
53	48173.5
54	42267.0
55	39473.0
56	36679.0
57	34567.0
58	32455.0
59	29574.0
60	26693.0
61	24155.0
62	21617.0
63	18684.5
64	15752.0
65	12904.0
66	10056.0
67	8905.0
68	7754.0
69	6635.5
70	5517.0
71	4613.0
72	3709.0
73	2803.5
74	1898.0
75	1440.5
76	983.0
77	790.0
78	597.0
79	430.5
80	264.0
81	184.5
82	105.0
83	80.0
84	55.0
85	39.0
86	23.0
87	16.5
88	10.0
89	5.0
90	0.0
91	1.0
92	2.0
93	1.5
94	1.0
95	1.0
96	1.0
97	0.5
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
50	890969.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	22.516316238631727
#Duplication Level	Percentage of deduplicated	Percentage of total
1	73.35217287739903	16.516207212983012
2	6.183249618280342	2.784480075751982
3	2.6712525865222823	1.8044030397419604
4	1.8007248011067984	1.6218275632187158
5	1.3727302830850796	1.5454414582145053
6	1.1314846746270777	1.5286120051841163
7	0.9238418525566816	1.4561060714654739
8	0.8213612335389111	1.4795223424411819
9	0.7553339289732272	1.530660384947644
>10	9.729736927828457	48.97285761849034
>50	1.1627290719705383	16.76536154009323
>100	0.09279023802156768	2.834320365069751
>500	5.183812179975848E-4	0.0740007046680485
>1k	0.002073524871990339	1.0861996177300624
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CGTTTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	3664	0.41123765248847044	No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATA	2522	0.2830625981375334	No Hit
CGTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1563	0.17542697894090592	No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1557	0.17475355483748595	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.007183190436479832	0.0
2	0.0	0.0	0.0	0.03557924013068917	0.0
3	0.0	0.0	0.0	0.05028233305535883	0.0
4	0.0	0.0	0.0	0.07183190436479833	0.0
5	0.0	0.0	0.0	0.11841040485134724	0.0
6	0.0	0.0	0.0	0.19933353461231534	0.0
7	0.0	0.0	0.0	0.2515239026273641	0.0
8	0.0	0.0	0.0	0.5502997298446972	0.0
9	0.0	0.0	0.0	0.6829642782184341	0.0
10	0.0	0.0	0.0	0.9155200685994687	0.0
11	0.0	0.0	0.0	1.1361786998200836	0.0
12	0.0	0.0	0.0	1.330686028357889	0.0
13	0.0	0.0	0.0	1.4131804810268371	0.0
14	0.0	0.0	0.0	1.4404541572153464	0.0
15	0.0	0.0	0.0	1.4870326577018953	0.0
16	0.0	0.0	0.0	1.6061164866566626	0.0
17	0.0	0.0	0.0	1.7374341868235594	0.0
18	0.0	0.0	0.0	1.9448488106769146	0.0
19	0.0	0.0	0.0	2.0201600729093827	0.0
20	0.0	0.0	0.0	2.1078174437045507	0.0
21	0.0	0.0	0.0	2.2111880435795186	0.0
22	0.0	0.0	0.0	2.3034471457480565	0.0
23	0.0	0.0	0.0	2.4179292433294535	0.0
24	0.0	0.0	0.0	2.5048009526706316	0.0
25	0.0	0.0	0.0	2.56361332436931	0.0
26	0.0	0.0	0.0	2.628149800947059	0.0
27	0.0	0.0	0.0	2.6927985148753772	0.0
28	0.0	0.0	0.0	2.755314719142866	0.0
29	0.0	0.0	0.0	2.828156759662794	0.0
30	0.0	0.0	0.0	2.923558507647292	0.0
31	0.0	0.0	0.0	3.0135728628044296	0.0
32	0.0	0.0	0.0	3.092587957605708	0.0
33	0.0	0.0	0.0	3.1703684415507163	0.0
34	0.0	0.0	0.0	3.2485978748980044	0.0
35	0.0	0.0	0.0	3.3617331242725617	0.0
36	0.0	0.0	0.0	3.4432174407863796	0.0
37	0.0	0.0	0.0	3.5346908815009277	0.0
38	0.0	0.0	0.0	3.6114612292908057	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GACCGTT	30	2.5270729E-6	44.000004	9
TCCCTAG	20	7.8555266E-4	44.000004	2
TAGCCGT	20	7.8555266E-4	44.000004	44
TACGCTT	20	7.8555266E-4	44.000004	23
TACGCAA	30	2.5270729E-6	44.000004	1
GCCGAAC	20	7.8555266E-4	44.000004	31
AAGTTCG	20	7.8555266E-4	44.000004	31
TACGAGT	20	7.8555266E-4	44.000004	27
TGTTACG	20	7.8555266E-4	44.000004	1
TCATCGG	20	7.8555266E-4	44.000004	44
TATAGCG	20	7.8555266E-4	44.000004	1
CGTAAAT	60	0.0	44.000004	16
TTACGCT	20	7.8555266E-4	44.000004	22
CGAGACA	60	0.0	44.000004	22
GTCCAAT	20	7.8555266E-4	44.000004	9
GCGTAAA	30	2.5270729E-6	44.000004	1
TCGCCGA	25	4.4420318E-5	44.0	43
TTTACGG	90	0.0	44.0	2
GCGAATA	25	4.4420318E-5	44.0	1
ACGTTAG	50	2.7284841E-11	44.0	2
>>END_MODULE