FastQCFastQC Report
Thu 26 May 2016
SRR1545713_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1545713_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences341731
Sequences flagged as poor quality0
Sequence length51
%GC44

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATAC10090.2952614775949504No Hit
CGTTTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT9840.28794578191618553No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT9200.26921760097854747No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTTAGGC453.8380676E-1045.0000044
ATAAGGT453.8380676E-1045.0000044
TATAGAG453.8380676E-1045.0000042
GTTGGTA302.1610085E-645.00000427
TGCACGG302.1610085E-645.0000042
TCACGAG207.026321E-445.02
CTAGAGC207.026321E-445.040
TGATATC207.026321E-445.05
GTACCTT253.8850332E-545.032
TCACCAC253.8850332E-545.011
GATCCAT253.8850332E-545.09
GATTTCG207.026321E-445.011
AAGGTCC406.7902874E-945.06
CAATTAG406.7902874E-945.02
TACGGGA253.8850332E-545.04
AGGCGAT2800.045.07
GTAGCCG253.8850332E-545.043
ATTCAGC207.026321E-445.041
CGAGGTT207.026321E-445.05
GTGTAAG850.045.02