FastQCFastQC Report
Thu 26 May 2016
SRR1545712_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1545712_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences447301
Sequences flagged as poor quality0
Sequence length50
%GC44

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT28830.6445324289460564No Hit
CGTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT11640.2602274531020498No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT11420.2553090648131795No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATA7230.1616361242206031No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCATCG207.8513176E-444.00000421
CGTTGAT207.8513176E-444.00000425
AGACGCA207.8513176E-444.00000428
TATTACG207.8513176E-444.0000041
ATGCGAA207.8513176E-444.0000041
CGGTCTA650.044.00000431
TAGGCCG207.8513176E-444.0000046
TGTAACG207.8513176E-444.0000041
AGTCGAC207.8513176E-444.00000412
CGAACGG254.4384666E-544.02
ATTACGG351.4434227E-744.02
ATTCGGC302.524237E-644.017
CCGCTCG302.524237E-644.019
ACGACGG600.044.027
TCACGTC302.524237E-644.039
CGTTTTA14400.042.6249961
CGTTATT9750.042.4205131
AGGCGAT3350.042.029857
CGTTTTT8250.041.0666661
CGACGGT700.040.85714328
Produced by FastQC (version 0.11.5)