##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1545712_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	447301
Sequences flagged as poor quality	0
Sequence length	50
%GC	44
>>END_MODULE
>>Per base sequence quality	fail
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.547874920914552	31.0	31.0	33.0	30.0	34.0
2	31.73514255501329	31.0	31.0	34.0	30.0	34.0
3	31.737022720718265	31.0	31.0	34.0	30.0	34.0
4	35.658726450421526	37.0	35.0	37.0	33.0	37.0
5	27.108676260504673	35.0	25.0	37.0	0.0	37.0
6	31.346878276596744	35.0	26.0	37.0	17.0	37.0
7	14.999246592339386	0.0	0.0	32.0	0.0	37.0
8	25.091195861399818	17.0	17.0	35.0	17.0	37.0
9	33.150518331056716	32.0	32.0	37.0	28.0	39.0
10	36.024457803581925	37.0	35.0	39.0	32.0	39.0
11	37.04050292755885	39.0	37.0	39.0	34.0	39.0
12	37.089742701223564	39.0	35.0	39.0	34.0	39.0
13	36.681740036351364	39.0	35.0	39.0	33.0	39.0
14	38.01350097585295	40.0	37.0	41.0	33.0	41.0
15	38.258564143608	40.0	37.0	41.0	33.0	41.0
16	38.29125354068066	40.0	37.0	41.0	34.0	41.0
17	38.29898435281835	40.0	37.0	41.0	34.0	41.0
18	38.26475460595885	40.0	37.0	41.0	34.0	41.0
19	38.196241457094885	40.0	37.0	41.0	34.0	41.0
20	38.20244756886302	40.0	36.0	41.0	34.0	41.0
21	38.07662401827852	40.0	36.0	41.0	34.0	41.0
22	37.9523162255394	40.0	36.0	41.0	34.0	41.0
23	37.84010990362194	40.0	35.0	41.0	34.0	41.0
24	37.66322454007481	39.0	35.0	41.0	33.0	41.0
25	37.48188356386415	39.0	35.0	41.0	33.0	41.0
26	37.23567798864747	39.0	35.0	40.0	33.0	41.0
27	37.027621221504084	39.0	35.0	40.0	33.0	41.0
28	37.037990078269445	39.0	35.0	40.0	33.0	41.0
29	36.972968985090574	39.0	35.0	40.0	33.0	41.0
30	36.72250229711089	38.0	35.0	40.0	32.0	41.0
31	36.51381955327621	38.0	35.0	40.0	31.0	41.0
32	36.21959262331182	38.0	35.0	40.0	31.0	41.0
33	35.934594378282185	38.0	35.0	40.0	30.0	41.0
34	35.64108508588177	38.0	35.0	40.0	29.0	41.0
35	35.401418731458236	38.0	35.0	40.0	27.0	41.0
36	35.20699260676815	38.0	35.0	40.0	26.0	41.0
37	35.0452983561405	38.0	34.0	40.0	25.0	41.0
38	34.887874160799996	38.0	34.0	40.0	25.0	41.0
39	34.83092146004592	38.0	34.0	40.0	24.0	41.0
40	34.58857011274287	38.0	34.0	40.0	23.0	41.0
41	34.41195526055162	37.0	34.0	40.0	23.0	40.0
42	34.39399643640412	37.0	34.0	40.0	23.0	41.0
43	34.283690400871	37.0	33.0	40.0	23.0	41.0
44	34.06143961225215	37.0	33.0	40.0	23.0	40.0
45	33.86641657407428	37.0	33.0	40.0	23.0	40.0
46	33.81195213066816	36.0	33.0	39.0	23.0	40.0
47	33.633716445972624	36.0	33.0	39.0	22.0	40.0
48	33.52446339265953	36.0	33.0	39.0	22.0	40.0
49	33.41773213116	36.0	33.0	39.0	21.0	40.0
50	33.497251291635834	36.0	33.0	39.0	21.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
12	2.0
13	2.0
14	8.0
15	11.0
16	23.0
17	88.0
18	149.0
19	365.0
20	687.0
21	1168.0
22	1942.0
23	2982.0
24	4511.0
25	6703.0
26	8829.0
27	8966.0
28	8547.0
29	9092.0
30	10656.0
31	14043.0
32	19260.0
33	34033.0
34	36286.0
35	45587.0
36	61137.0
37	111941.0
38	47835.0
39	12448.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	35.11952801357475	21.64806249035884	26.13676249326516	17.095647002801247
2	37.259921171649516	23.21322778173981	27.061866617780865	12.464984428829803
3	30.198233404351882	23.232230645583176	33.832698786723036	12.73683716334191
4	26.42337039264388	25.870945962562125	32.648261461521436	15.057422183272562
5	16.888180442252533	47.00660181846229	24.542086872150968	11.563130867134213
6	20.018734588118512	39.13896906110203	31.04397262693354	9.798323723845911
7	37.167813172785216	55.962986892495216	5.690351687118965	1.178848247600609
8	82.44224806114899	3.958631883228519	11.38539819942276	2.2137218561997405
9	77.571255150335	5.6244005714272935	13.300886874833726	3.503457403403972
10	45.20356538438322	23.156666316417805	19.411760760651106	12.228007538547867
11	38.92658411226445	22.554387314135223	24.91342518796068	13.605603385639647
12	34.85684136632827	21.67377224732339	29.124906941857944	14.3444794444904
13	22.3513920156673	33.32297490951283	28.936890371360676	15.38874270345919
14	16.68987996896944	36.679998479770894	31.42581840863311	15.204303142626555
15	16.323236478344562	22.969543998336693	45.14476828802082	15.56245123529793
16	18.070158573309694	19.219720054281122	44.18724751341937	18.522873858989808
17	18.528686499694835	18.88929378651065	32.401447794661756	30.180571919132753
18	21.65812283004062	22.1682938334589	36.326545212284344	19.847038124216134
19	27.7198128329693	23.284097285720353	29.29570915334417	19.70038072796618
20	29.19354081479809	23.03214166746777	28.666379015472803	19.10793850226134
21	23.181481820966194	25.644700101274086	31.63060221193335	19.54321586582637
22	22.807907874116086	22.97177962937709	29.968410533399208	24.251901963107617
23	20.35229073934554	27.334613604709133	29.138097164996278	23.174998490949047
24	20.905162295635378	23.420023652976408	37.88902774641684	17.785786304971374
25	19.95121853069857	23.678462601246142	36.1132660110306	20.25705285702469
26	18.001971826577627	30.163133997017667	31.226847246037902	20.6080469303668
27	18.946302378040738	29.258150551865526	32.58901723895095	19.206529831142788
28	17.323010679609478	26.611386963141154	38.24404595563167	17.821556401617702
29	18.39477220037514	24.058296315009358	37.3768446750622	20.170086809553297
30	20.15533164468669	26.839644892365545	33.88367117444405	19.121352288503715
31	27.209418266446978	25.206739980460586	28.628596850890116	18.95524490220232
32	27.17342460669661	25.71556960525463	29.456003898940537	17.65500188910823
33	26.64313292391477	26.142798697074234	28.088021265322453	19.126047113688546
34	20.65879575498378	27.173871732904686	30.17610065705196	21.991231855059567
35	20.854189907914357	25.857755739423787	31.470307466336983	21.81774688632487
36	27.747087531662125	25.82600977865017	27.877424821317188	18.549477868370516
37	21.53516312281886	30.26753796660414	28.85685478011451	19.340444130462487
38	21.115535176536604	30.702144640857053	26.032358523678685	22.14996165892766
39	21.430088463920267	29.143462679493226	28.11127182814257	21.315177028443934
40	23.299970266107163	25.55035647136939	28.26955450580258	22.880118756720865
41	19.273151636146576	25.442375492118284	28.837628353167105	26.446844518568035
42	22.395881073371175	26.736805864507346	26.27581874397777	24.59149431814371
43	22.230891502589976	26.549012857114114	27.817957035642664	23.402138604653242
44	20.872522082445602	28.715562898361508	29.39944243361853	21.01247258557437
45	19.448648672817633	32.65452122843455	25.874970098434837	22.021860000312987
46	22.406388539261034	29.787324419127163	27.79694210386295	20.009344937748853
47	21.31718909638029	28.505637143668356	28.202038448382634	21.97513531156872
48	22.391409811290384	26.26888828775254	30.214777074050808	21.124924826906266
49	21.844127332601538	25.171864136230415	31.134068557861482	21.849939973306565
50	20.273820089827655	30.135188609012726	28.81504847965911	20.77594282150051
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	365.0
1	593.5
2	822.0
3	4729.5
4	8637.0
5	6139.5
6	3642.0
7	3766.0
8	3890.0
9	3956.5
10	4023.0
11	3974.0
12	3925.0
13	3705.5
14	3486.0
15	3325.0
16	3164.0
17	3029.5
18	2895.0
19	2843.5
20	2792.0
21	2847.0
22	2902.0
23	2987.5
24	3073.0
25	3434.5
26	3796.0
27	4570.0
28	5344.0
29	6175.0
30	7006.0
31	7904.0
32	8802.0
33	9888.0
34	10974.0
35	11967.0
36	12960.0
37	15325.0
38	17690.0
39	21840.0
40	25990.0
41	30112.5
42	34235.0
43	35684.5
44	37134.0
45	37217.5
46	37301.0
47	35827.0
48	34353.0
49	33040.5
50	31728.0
51	28860.5
52	25993.0
53	23856.0
54	21719.0
55	20523.0
56	19327.0
57	18436.5
58	17546.0
59	16355.5
60	15165.0
61	13452.0
62	11739.0
63	9775.5
64	7812.0
65	6754.0
66	5696.0
67	4924.0
68	4152.0
69	3571.5
70	2991.0
71	2497.0
72	2003.0
73	1534.0
74	1065.0
75	808.0
76	551.0
77	439.5
78	328.0
79	250.5
80	173.0
81	122.5
82	72.0
83	45.5
84	19.0
85	14.5
86	10.0
87	7.5
88	5.0
89	4.0
90	3.0
91	2.5
92	2.0
93	1.5
94	1.0
95	0.5
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
50	447301.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	36.84328983788677
#Duplication Level	Percentage of deduplicated	Percentage of total
1	65.75720939004411	24.22711924488005
2	9.526055029941585	7.019424129595938
3	5.569164124565488	6.155589839883817
4	3.9082917392812604	5.759773012854323
5	2.9793512987218245	5.4884551713846275
6	2.2649726276008346	5.006942579614652
7	1.8175113678357853	4.687416867617951
8	1.3786682996184267	4.063574060251858
9	1.1599032174677484	3.8461185382555487
>10	5.5981654889871395	31.23694486799358
>50	0.03243872207384219	0.8218408592854202
>100	0.005724480365972152	0.30136508270346457
>500	6.360533739969058E-4	0.16942998040015933
>1k	0.0019081601219907175	1.2160057652785985
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CGTTTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	2883	0.6445324289460564	No Hit
CGTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1164	0.2602274531020498	No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1142	0.2553090648131795	No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATA	723	0.1616361242206031	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.010954592097938524	0.0
2	0.0	0.0	0.0	0.03755860147864637	0.0
3	0.0	0.0	0.0	0.052537329449297	0.0
4	0.0	0.0	0.0	0.06505686327551247	0.0
5	0.0	0.0	0.0	0.10060339681780278	0.0
6	0.0	0.0	0.0	0.16007118249232619	0.0
7	0.0	0.0	0.0	0.19673553155481432	0.0
8	0.0	0.0	0.0	0.3760331409945428	0.0
9	0.0	0.0	0.0	0.45226815947203336	0.0
10	0.0	0.0	0.0	0.5917715363927198	0.0
11	0.0	0.0	0.0	0.7630208740870241	0.0
12	0.0	0.0	0.0	0.8893340278693765	0.0
13	0.0	0.0	0.0	0.9543908911448891	0.0
14	0.0	0.0	0.0	0.9865839781265859	0.0
15	0.0	0.0	0.0	1.0230247640850345	0.0
16	0.0	0.0	0.0	1.1126735688048988	0.0
17	0.0	0.0	0.0	1.2061229462934355	0.0
18	0.0	0.0	0.0	1.3422728766535286	0.0
19	0.0	0.0	0.0	1.4100124971775159	0.0
20	0.0	0.0	0.0	1.477304991493424	0.0
21	0.0	0.0	0.0	1.5669537962132882	0.0
22	0.0	0.0	0.0	1.6523549019564008	0.0
23	0.0	0.0	0.0	1.756311745334797	0.0
24	0.0	0.0	0.0	1.8381358414132765	0.0
25	0.0	0.0	0.0	1.9038633940009078	0.0
26	0.0	0.0	0.0	1.9738386455652905	0.0
27	0.0	0.0	0.0	2.047167343690267	0.0
28	0.0	0.0	0.0	2.1222845466475593	0.0
29	0.0	0.0	0.0	2.194495429252338	0.0
30	0.0	0.0	0.0	2.2861563019085582	0.0
31	0.0	0.0	0.0	2.378040737668818	0.0
32	0.0	0.0	0.0	2.4634418434119305	0.0
33	0.0	0.0	0.0	2.541912492929817	0.0
34	0.0	0.0	0.0	2.6284314141931273	0.0
35	0.0	0.0	0.0	2.744907791397739	0.0
36	0.0	0.0	0.0	2.8363451009499197	0.0
37	0.0	0.0	0.0	2.9329243618950103	0.0
38	0.0	0.0	0.0	3.036657642169367	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGCATCG	20	7.8513176E-4	44.000004	21
CGTTGAT	20	7.8513176E-4	44.000004	25
AGACGCA	20	7.8513176E-4	44.000004	28
TATTACG	20	7.8513176E-4	44.000004	1
ATGCGAA	20	7.8513176E-4	44.000004	1
CGGTCTA	65	0.0	44.000004	31
TAGGCCG	20	7.8513176E-4	44.000004	6
TGTAACG	20	7.8513176E-4	44.000004	1
AGTCGAC	20	7.8513176E-4	44.000004	12
CGAACGG	25	4.4384666E-5	44.0	2
ATTACGG	35	1.4434227E-7	44.0	2
ATTCGGC	30	2.524237E-6	44.0	17
CCGCTCG	30	2.524237E-6	44.0	19
ACGACGG	60	0.0	44.0	27
TCACGTC	30	2.524237E-6	44.0	39
CGTTTTA	1440	0.0	42.624996	1
CGTTATT	975	0.0	42.420513	1
AGGCGAT	335	0.0	42.02985	7
CGTTTTT	825	0.0	41.066666	1
CGACGGT	70	0.0	40.857143	28
>>END_MODULE