FastQCFastQC Report
Thu 26 May 2016
SRR1545711_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1545711_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences171241
Sequences flagged as poor quality0
Sequence length51
%GC44

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT7550.440899083747467No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT6830.39885307840996026No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATAC2830.16526415986825585No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGGCGAT1200.045.0000047
GTATAAG302.154653E-645.0000042
GTTGATC700.045.016
ATGGGCG351.2038436E-745.05
TACGGGA207.016458E-445.04
CTGGTTG700.045.013
CAAGCGG453.8016879E-1045.021
CTACATA207.016458E-445.039
GCCAGTA700.045.025
TAGGAAC351.2038436E-745.06
CTAGGCG207.016458E-445.05
ACAAGCC207.016458E-445.045
GTTATAA207.016458E-445.01
CTCGTCC253.8768638E-544.99999637
CATAGGC253.8768638E-544.9999964
TATAGGC253.8768638E-544.9999964
CGGTCTA253.8768638E-544.99999631
ACGACGG253.8768638E-544.99999627
CGTTTTA4650.044.032261
AAGGCGA800.042.18756