##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1545711_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	171241
Sequences flagged as poor quality	0
Sequence length	51
%GC	44
>>END_MODULE
>>Per base sequence quality	fail
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.01946963636045	31.0	31.0	33.0	30.0	33.0
2	31.1884186614187	31.0	31.0	33.0	30.0	34.0
3	31.074625819751112	31.0	31.0	34.0	28.0	34.0
4	35.04672946315427	35.0	35.0	37.0	32.0	37.0
5	32.830303490402414	35.0	35.0	37.0	28.0	37.0
6	34.01637458318977	35.0	35.0	37.0	28.0	37.0
7	15.364206002067261	0.0	0.0	35.0	0.0	37.0
8	24.943634993955886	17.0	17.0	35.0	17.0	37.0
9	32.64729241244795	32.0	32.0	37.0	27.0	39.0
10	35.47940621696907	37.0	34.0	37.0	32.0	39.0
11	36.49144188599693	37.0	35.0	39.0	32.0	39.0
12	36.41393708282479	37.0	35.0	39.0	32.0	39.0
13	36.178987508832584	38.0	35.0	39.0	32.0	39.0
14	37.2319713152808	39.0	36.0	40.0	32.0	41.0
15	37.53718443597036	39.0	36.0	41.0	32.0	41.0
16	37.55270641960745	39.0	36.0	41.0	32.0	41.0
17	37.499494863963655	39.0	36.0	41.0	32.0	41.0
18	37.3866246985243	39.0	36.0	40.0	32.0	41.0
19	37.315917332881725	39.0	36.0	40.0	32.0	41.0
20	37.392879041818254	39.0	35.0	40.0	32.0	41.0
21	37.27298368965376	39.0	35.0	40.0	32.0	41.0
22	37.28800345711599	39.0	35.0	40.0	32.0	41.0
23	37.26010710051915	39.0	35.0	40.0	32.0	41.0
24	37.074520704737765	39.0	35.0	40.0	32.0	41.0
25	36.82214539742235	38.0	35.0	40.0	31.0	41.0
26	36.70286321616903	38.0	35.0	40.0	31.0	41.0
27	36.60745382239067	38.0	35.0	40.0	31.0	41.0
28	36.50193002843945	38.0	35.0	40.0	31.0	41.0
29	36.503711143943335	38.0	35.0	40.0	31.0	41.0
30	36.2015697175326	38.0	35.0	40.0	30.0	41.0
31	36.05009314358127	38.0	35.0	40.0	30.0	41.0
32	35.88367271856623	38.0	35.0	40.0	30.0	41.0
33	35.63883649359674	38.0	34.0	40.0	28.0	41.0
34	35.40023709275232	38.0	34.0	40.0	26.0	41.0
35	35.15754404611045	38.0	34.0	40.0	25.0	41.0
36	34.94508908497381	38.0	34.0	40.0	24.0	41.0
37	34.80691539993343	38.0	34.0	40.0	24.0	41.0
38	34.68738211059267	38.0	34.0	40.0	23.0	41.0
39	34.6092582967864	38.0	34.0	40.0	23.0	41.0
40	34.463492971893416	38.0	33.0	40.0	23.0	41.0
41	34.31602244789507	38.0	33.0	40.0	23.0	41.0
42	34.26012461968804	38.0	33.0	40.0	22.0	41.0
43	34.17242366022156	37.0	33.0	40.0	22.0	41.0
44	33.86256795977599	37.0	33.0	40.0	20.0	41.0
45	33.82612808848348	37.0	33.0	40.0	20.0	41.0
46	33.80330645114196	37.0	33.0	40.0	20.0	41.0
47	33.74818530608908	37.0	33.0	40.0	20.0	41.0
48	33.71710046075414	37.0	33.0	40.0	20.0	41.0
49	33.62004426510006	36.0	33.0	40.0	20.0	41.0
50	33.48816580141438	36.0	33.0	40.0	20.0	41.0
51	33.32814571276739	36.0	32.0	40.0	20.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
13	2.0
14	1.0
15	5.0
16	27.0
17	53.0
18	106.0
19	227.0
20	401.0
21	628.0
22	1002.0
23	1470.0
24	2145.0
25	2878.0
26	3416.0
27	3644.0
28	3662.0
29	4063.0
30	4828.0
31	6298.0
32	8196.0
33	12108.0
34	15335.0
35	17110.0
36	20650.0
37	30833.0
38	26504.0
39	5649.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	34.958917548951476	22.259855992431717	26.726076114949105	16.055150343667695
2	36.52104344169913	23.220490419934478	27.71882901875135	12.539637119615046
3	30.933596510181555	23.013180254728717	33.15035534714233	12.902867887947394
4	27.027405819867905	26.42766627151208	31.144410509165443	15.40051739945457
5	20.88693712370285	35.093815149409316	29.589876256270404	14.429371470617433
6	20.249239376084	39.9366974030752	30.20479908433144	9.609264136509363
7	36.262343714414186	56.90868425201908	5.943670032293668	0.8853020012730596
8	83.34277421879106	4.222119702641307	10.350908952879276	2.0841971256883576
9	78.1588521440543	5.912719500586892	12.308384090258759	3.6200442651000633
10	45.516552694740156	23.798622993325196	18.44301306346027	12.241811248474372
11	39.55711541044493	23.034203257397472	24.031043967274194	13.37763736488341
12	35.22462494379266	22.33518841866142	28.367622240000934	14.072564397544982
13	22.6686365998797	34.31070829999825	27.729340520085728	15.291314580036323
14	16.717374927733427	37.68490022833317	30.660881447784117	14.936843396149287
15	16.375751134366183	23.518900263371506	44.5010248713801	15.604323730882207
16	18.189569086842518	19.67519460876776	43.46914582372212	18.666090480667595
17	18.545208215322265	19.384960377479693	31.64020298877021	30.42962841842783
18	22.0519618549296	22.40935290029841	35.60537488101564	19.933310363756345
19	28.378717713631662	23.480358091812125	28.582524045059305	19.558400149496908
20	29.77032369584387	23.382834718320964	27.83445553342949	19.012386052405674
21	23.40444169328607	26.278169363645386	30.757820848978923	19.559568094089617
22	22.755064499740133	23.642118417902257	29.37555842350839	24.227258658849223
23	20.267342517270983	27.871829760396167	28.48792053304991	23.37290718928294
24	20.70415379494397	23.98140632208408	37.56752179676596	17.74691808620599
25	19.814180015300074	24.227258658849223	35.70348222680316	20.255079099047542
26	17.90867841229612	31.053894803230538	30.47751414672888	20.559912637744464
27	18.91895048498899	30.061725871724644	31.987666505100997	19.031657138185366
28	17.186888654002257	27.34625469367733	37.7789197680462	17.68793688427421
29	18.193072920620644	24.735898528973784	36.83755642632314	20.233472124082432
30	20.32865960838818	27.62948125740915	33.13166823365899	18.91019090054368
31	27.794745417277404	25.65799078491716	27.75036352275448	18.79690027505095
32	27.7807300821649	26.02063758095316	28.769395179892665	17.42923715698927
33	27.353262361233583	26.388540127656345	27.220116677664812	19.03808083344526
34	20.758463218504914	27.36786166864244	29.47308179699955	22.400593315853097
35	21.006651444455475	26.03465291606566	31.31376247510818	21.644933164370684
36	28.199438218650908	25.932457764203665	27.50509515828569	18.363008858859736
37	21.517627203765453	30.97389059863	28.52704667690565	18.9814355206989
38	20.864162204145035	31.347632868296728	25.62061655795049	22.167588369607746
39	21.658948499483184	29.63951390146051	27.786569805128448	20.914967793927854
40	23.531163681594943	25.594337804614547	27.94073849136597	22.933760022424536
41	19.18407390753383	25.57389877424215	28.525878732312936	26.716148585911085
42	22.130798114937427	26.68811791568608	25.78938455159687	25.391699417779623
43	22.169924258793163	26.476135972109482	27.655176038448737	23.69876373064862
44	20.493923768256433	29.10167541651824	29.276283133128167	21.128117682097162
45	19.308460006657285	33.543952674885105	25.499150320308807	21.648436998148807
46	22.30949363762183	30.34028065708563	27.35968605649348	19.99053964879906
47	21.111182485502887	29.078900496960426	27.455457513095578	22.35445950444111
48	22.120870585899404	26.6840301096116	30.32976915575125	20.865330148737744
49	21.716177784525904	25.722811709812486	31.118131755829502	21.442878749832108
50	20.206025426153783	31.118715728125856	28.255499559100915	20.419759286619442
51	19.94265392049801	31.599324928025414	26.716148585911085	21.74187256556549
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	159.0
1	228.5
2	298.0
3	1546.0
4	2794.0
5	2031.0
6	1268.0
7	1273.0
8	1278.0
9	1298.0
10	1318.0
11	1295.0
12	1272.0
13	1225.5
14	1179.0
15	1124.5
16	1070.0
17	1052.5
18	1035.0
19	1034.0
20	1033.0
21	1019.5
22	1006.0
23	1091.0
24	1176.0
25	1262.5
26	1623.0
27	1897.0
28	2193.0
29	2489.0
30	2847.0
31	3205.0
32	3670.0
33	4135.0
34	4467.5
35	4800.0
36	5572.0
37	6344.0
38	7656.5
39	8969.0
40	10643.0
41	12317.0
42	13232.5
43	14148.0
44	13978.0
45	13808.0
46	13530.5
47	13253.0
48	12674.5
49	12096.0
50	11442.0
51	10788.0
52	9893.5
53	8999.0
54	8421.0
55	7843.0
56	7395.5
57	6948.0
58	6550.0
59	6152.0
60	5499.0
61	4846.0
62	4268.0
63	3690.0
64	3087.5
65	2485.0
66	2158.5
67	1832.0
68	1615.5
69	1399.0
70	1239.0
71	1079.0
72	891.5
73	704.0
74	535.0
75	278.5
76	191.0
77	159.0
78	127.0
79	94.5
80	62.0
81	40.5
82	19.0
83	15.0
84	11.0
85	6.0
86	1.0
87	1.5
88	2.0
89	1.5
90	1.0
91	0.5
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	171241.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	50.453454488119085
#Duplication Level	Percentage of deduplicated	Percentage of total
1	66.952556223017	33.77987748261223
2	12.68678310589488	12.801840680678108
3	7.029179253909279	10.639391267278281
4	4.389041286155769	8.85769179110143
5	2.905193467365765	7.328852319245975
6	1.901686401148188	5.756798897460304
7	1.2546731946711112	4.431181784736133
8	0.9271155248446126	3.7420944750381038
9	0.6053450930009143	2.7487575989395063
>10	1.3403243168165562	8.682500102195153
>50	0.0023148951931201314	0.07942023230417948
>100	0.0034723427896801973	0.31184120625317535
>500	0.0023148951931201314	0.8397521621574273
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CGTTTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	755	0.440899083747467	No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	683	0.39885307840996026	No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATAC	283	0.16526415986825585	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.008175612148959654	0.0
2	0.0	0.0	0.0	0.030950531706775832	0.0
3	0.0	0.0	0.0	0.03971011615208975	0.0
4	0.0	0.0	0.0	0.05489339585730053	0.0
5	0.0	0.0	0.0	0.08993173363855618	0.0
6	0.0	0.0	0.0	0.13256171127241723	0.0
7	0.0	0.0	0.0	0.15183279705210784	0.0
8	0.0	0.0	0.0	0.2902342312880677	0.0
9	0.0	0.0	0.0	0.35855898996151625	0.0
10	0.0	0.0	0.0	0.47360153234330565	0.0
11	0.0	0.0	0.0	0.6073311882084313	0.0
12	0.0	0.0	0.0	0.7147820907376154	0.0
13	0.0	0.0	0.0	0.7661716528167903	0.0
14	0.0	0.0	0.0	0.7860267108928353	0.0
15	0.0	0.0	0.0	0.8204810763777366	0.0
16	0.0	0.0	0.0	0.8893898073475395	0.0
17	0.0	0.0	0.0	0.9658901781699476	0.0
18	0.0	0.0	0.0	1.085020526626217	0.0
19	0.0	0.0	0.0	1.1510093961142482	0.0
20	0.0	0.0	0.0	1.2111585426387372	0.0
21	0.0	0.0	0.0	1.277731384423123	0.0
22	0.0	0.0	0.0	1.3419683370220916	0.0
23	0.0	0.0	0.0	1.4336519875497107	0.0
24	0.0	0.0	0.0	1.5072324968903474	0.0
25	0.0	0.0	0.0	1.5632938373403567	0.0
26	0.0	0.0	0.0	1.61993915008672	0.0
27	0.0	0.0	0.0	1.6824241857966258	0.0
28	0.0	0.0	0.0	1.7384855262466348	0.0
29	0.0	0.0	0.0	1.8033064511419579	0.0
30	0.0	0.0	0.0	1.8868144895206171	0.0
31	0.0	0.0	0.0	1.9592270542685455	0.0
32	0.0	0.0	0.0	2.032223591312828	0.0
33	0.0	0.0	0.0	2.1087239621352363	0.0
34	0.0	0.0	0.0	2.198071723477438	0.0
35	0.0	0.0	0.0	2.3014348199321426	0.0
36	0.0	0.0	0.0	2.379103135347259	0.0
37	0.0	0.0	0.0	2.4579393953550843	0.0
38	0.0	0.0	0.0	2.5484551012899948	0.0
39	0.0	0.0	0.0	2.66700147744991	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
AGGCGAT	120	0.0	45.000004	7
GTATAAG	30	2.154653E-6	45.000004	2
GTTGATC	70	0.0	45.0	16
ATGGGCG	35	1.2038436E-7	45.0	5
TACGGGA	20	7.016458E-4	45.0	4
CTGGTTG	70	0.0	45.0	13
CAAGCGG	45	3.8016879E-10	45.0	21
CTACATA	20	7.016458E-4	45.0	39
GCCAGTA	70	0.0	45.0	25
TAGGAAC	35	1.2038436E-7	45.0	6
CTAGGCG	20	7.016458E-4	45.0	5
ACAAGCC	20	7.016458E-4	45.0	45
GTTATAA	20	7.016458E-4	45.0	1
CTCGTCC	25	3.8768638E-5	44.999996	37
CATAGGC	25	3.8768638E-5	44.999996	4
TATAGGC	25	3.8768638E-5	44.999996	4
CGGTCTA	25	3.8768638E-5	44.999996	31
ACGACGG	25	3.8768638E-5	44.999996	27
CGTTTTA	465	0.0	44.03226	1
AAGGCGA	80	0.0	42.1875	6
>>END_MODULE