FastQCFastQC Report
Thu 26 May 2016
SRR1545710_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1545710_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences363882
Sequences flagged as poor quality0
Sequence length50
%GC44

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT37821.0393479204797158No Hit
CGTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT17420.4787266201680765No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT15050.41359561616128304No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATTTACG207.849382E-444.01
CGCATCG207.849382E-444.021
CGCATAG207.849382E-444.02
GTTAGGC302.5229292E-644.04
TAACGGG454.783942E-1044.03
GCGTTAA207.849382E-444.01
TCGATAG207.849382E-444.02
ACGGGGC551.8189894E-1244.05
TACTCGG207.849382E-444.044
CATCGTT207.849382E-444.036
CGTAAAG254.4368247E-544.02
TAGGCGA502.7284841E-1144.06
GTAGTCC207.849382E-444.033
CGTTTTA17750.043.6281741
CGTTATT9750.043.548721
TAAGGAT1100.042.05
GTTTTAT19750.040.8810122
CGTTAGG603.6379788E-1240.3333323
CGGTCTA603.6379788E-1240.33333231
GTTATTT10700.040.2990652
Produced by FastQC (version 0.11.5)