##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1545710_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	363882
Sequences flagged as poor quality	0
Sequence length	50
%GC	44
>>END_MODULE
>>Per base sequence quality	fail
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.809561890942668	31.0	31.0	33.0	31.0	34.0
2	32.05236587685019	33.0	31.0	34.0	31.0	34.0
3	32.03779247118572	33.0	31.0	34.0	30.0	34.0
4	35.945012394127765	37.0	35.0	37.0	35.0	37.0
5	26.925800122017577	37.0	0.0	37.0	0.0	37.0
6	31.381508291149327	37.0	19.0	37.0	17.0	37.0
7	15.001951181976574	0.0	0.0	32.0	0.0	37.0
8	25.139264926542122	17.0	17.0	35.0	17.0	37.0
9	33.39611742268098	32.0	32.0	37.0	28.0	39.0
10	36.3723734617266	37.0	35.0	39.0	34.0	39.0
11	37.42455521295365	39.0	37.0	39.0	35.0	39.0
12	37.601285581589636	39.0	37.0	39.0	35.0	39.0
13	37.26172495479304	39.0	37.0	39.0	35.0	39.0
14	38.66811493835914	40.0	38.0	41.0	35.0	41.0
15	38.886160898313186	40.0	38.0	41.0	35.0	41.0
16	38.909281030663784	40.0	38.0	41.0	35.0	41.0
17	38.953259573158334	40.0	38.0	41.0	35.0	41.0
18	38.89886281816633	40.0	38.0	41.0	35.0	41.0
19	38.842536866346784	40.0	38.0	41.0	35.0	41.0
20	38.85295782698787	40.0	38.0	41.0	35.0	41.0
21	38.736601425736914	40.0	38.0	41.0	35.0	41.0
22	38.569080086401634	40.0	37.0	41.0	35.0	41.0
23	38.433761494110726	40.0	37.0	41.0	35.0	41.0
24	38.229423274578025	40.0	35.0	41.0	34.0	41.0
25	38.05371246722839	40.0	35.0	41.0	34.0	41.0
26	37.85402410671591	40.0	35.0	41.0	34.0	41.0
27	37.65268686002605	40.0	35.0	41.0	33.0	41.0
28	37.67778565578951	40.0	35.0	41.0	33.0	41.0
29	37.558406296546686	39.0	35.0	41.0	33.0	41.0
30	37.33012899786194	39.0	35.0	40.0	33.0	41.0
31	37.10392105132983	39.0	35.0	40.0	33.0	41.0
32	36.808822640306474	39.0	35.0	40.0	33.0	41.0
33	36.49869737991986	39.0	35.0	40.0	31.0	41.0
34	36.12759630869348	39.0	35.0	40.0	31.0	41.0
35	35.78418278452905	39.0	35.0	40.0	29.0	41.0
36	35.563490362260296	38.0	35.0	40.0	27.0	41.0
37	35.44988210463832	38.0	35.0	40.0	27.0	41.0
38	35.25425000412221	38.0	35.0	40.0	26.0	41.0
39	35.157570311254744	38.0	35.0	40.0	25.0	41.0
40	34.92101010767227	38.0	35.0	40.0	24.0	41.0
41	34.692018291644	38.0	34.0	40.0	23.0	41.0
42	34.67444116499305	38.0	34.0	40.0	23.0	41.0
43	34.56238011223419	38.0	34.0	40.0	23.0	41.0
44	34.31528077783457	37.0	34.0	40.0	23.0	40.0
45	34.11617502377144	37.0	34.0	40.0	23.0	40.0
46	34.06302317784337	37.0	34.0	40.0	23.0	40.0
47	33.92298052665424	36.0	33.0	39.0	23.0	40.0
48	33.81855931318395	36.0	33.0	39.0	23.0	40.0
49	33.66716133251988	36.0	33.0	39.0	22.0	40.0
50	33.76919715732023	36.0	33.0	39.0	22.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
11	2.0
12	0.0
13	2.0
14	2.0
15	9.0
16	12.0
17	31.0
18	80.0
19	147.0
20	288.0
21	598.0
22	1057.0
23	1825.0
24	3244.0
25	5337.0
26	6898.0
27	7126.0
28	6357.0
29	5790.0
30	6241.0
31	8135.0
32	11803.0
33	22288.0
34	26119.0
35	37189.0
36	55421.0
37	102549.0
38	43312.0
39	12020.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	33.36988364359875	21.199179953941112	25.572575725097696	19.85836067736244
2	39.96790168241353	21.83565001841256	26.467096476330237	11.729351822843668
3	29.31994437757295	22.71258265041964	35.663759130707206	12.303713841300201
4	26.09499782896653	25.076260985704156	34.131119428825826	14.697621756503482
5	16.355027179140492	47.216680132570445	25.518162481243923	10.91013020704514
6	19.917445765385484	37.85183108809999	32.37395639245689	9.856766754057634
7	36.50798885352944	55.93544060986804	6.499359682534448	1.057210854068077
8	81.03121341533794	3.425561033521856	13.529660714187566	2.0135648369526384
9	75.85288637525352	5.294298701227321	15.262640086621488	3.590174836897676
10	40.747550029954766	24.06411968715133	21.96453795461166	13.223792328282247
11	33.559230739635375	23.142667128354795	27.975827328639504	15.322274803370323
12	29.667859361001643	21.034840964928193	33.05687008425808	16.240429589812084
13	22.9109986204319	26.794125568178696	33.276996388939274	17.017879422450136
14	18.93031257385636	29.3518228436691	35.2399953831187	16.477869199355837
15	18.04321181042206	22.497128189907716	41.639047823195426	17.82061217647479
16	21.082383849709522	19.36122149488021	39.900572163503554	19.655822491906715
17	21.19863032521532	19.401619206226194	33.728241572817566	25.671508895740924
18	22.48283784303703	22.174496127865627	35.45325132872744	19.8894147003699
19	26.234878339681543	23.338334954738073	30.88308847373599	19.543698231844388
20	26.742460467953897	23.318823134972327	30.544242364282926	19.39447403279085
21	24.49337972199779	23.443588855727956	33.34899775201851	18.71403367025574
22	23.120681979322967	21.17142370328843	32.55835682996136	23.149537487427242
23	21.756778296260876	24.699490494171187	31.304653706421316	22.239077503146625
24	21.08128459225793	23.94567469674235	35.94242089468564	19.03061981631408
25	22.225886413727526	22.858509077118406	34.2633051373797	20.652299371774365
26	19.71353350811527	26.055974189435037	31.785578841492573	22.44491346095712
27	19.637959558318354	25.06032175265608	33.94809306313585	21.35362562588971
28	19.5035753348613	24.415882071660594	36.02871260463557	20.051829988842538
29	20.14911427330838	24.428248717990996	35.20729247393386	20.215344534766764
30	22.212970138671327	24.2221379458176	34.50101956128635	19.06387235422472
31	25.707234762917647	23.92423917643632	30.838018918220744	19.53050714242529
32	25.576697940541166	24.41615688602349	30.341154550101407	19.665990623333936
33	24.95644192348069	24.5178382002957	29.666210474824258	20.859509401399354
34	21.272555388834842	25.816061250625204	31.33955513050934	21.571828230030615
35	21.867803298871614	25.603080119379356	31.176040584585113	21.353075997163913
36	26.220587992810856	25.809465705915652	27.92855925822107	20.04138704305242
37	21.553965296442254	28.93025761098378	28.842591829219362	20.673185263354604
38	23.019550293776554	28.34545264673713	27.5130399415195	21.121957117966815
39	22.210771623768146	27.069214745439456	27.564430227381404	23.155583403410994
40	23.536476110387433	24.889936847659406	29.34385322714506	22.229733814808096
41	20.863081988117028	24.908624224336464	29.620041661857417	24.6082521256891
42	22.21406939612292	26.910097229321593	27.95329255088188	22.92254082367361
43	22.020874899005722	26.02354609461309	28.98989232773262	22.965686678648574
44	22.190160546550803	26.57619777840069	29.948444825520358	21.28519684952814
45	21.105468256192943	28.748605317108293	28.031065015581973	22.11486141111679
46	22.272330041057266	27.140391665430002	29.7247459341215	20.86253235939123
47	21.67405917302862	27.60098053764682	29.300157743444306	21.424802545880258
48	21.651524395270993	27.479237774883064	29.79152582430568	21.07771200554026
49	22.41715721030444	25.913895163816843	30.47334025865528	21.19560736722344
50	21.26156281431893	27.08515397848753	29.660989001929195	21.992294205264344
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	327.0
1	556.5
2	786.0
3	5941.0
4	11096.0
5	7496.0
6	3896.0
7	3882.0
8	3868.0
9	3836.5
10	3805.0
11	3763.5
12	3722.0
13	3481.0
14	3240.0
15	3051.5
16	2863.0
17	2733.5
18	2604.0
19	2527.5
20	2451.0
21	2501.0
22	2551.0
23	2658.0
24	2765.0
25	2985.0
26	3205.0
27	3647.0
28	4089.0
29	4838.0
30	5587.0
31	6436.5
32	7286.0
33	8203.0
34	9120.0
35	9770.5
36	10421.0
37	11412.0
38	12403.0
39	14698.0
40	16993.0
41	18793.0
42	20593.0
43	21522.0
44	22451.0
45	23396.0
46	24341.0
47	24770.5
48	25200.0
49	24350.0
50	23500.0
51	22089.5
52	20679.0
53	19827.5
54	18976.0
55	18997.5
56	19019.0
57	18410.0
58	17801.0
59	16450.5
60	15100.0
61	13708.0
62	12316.0
63	10825.5
64	9335.0
65	8120.5
66	6906.0
67	6042.0
68	5178.0
69	4488.0
70	3798.0
71	3201.5
72	2605.0
73	2039.0
74	1473.0
75	1118.5
76	764.0
77	568.0
78	372.0
79	283.5
80	195.0
81	155.5
82	116.0
83	80.5
84	45.0
85	31.0
86	17.0
87	11.0
88	5.0
89	5.0
90	5.0
91	5.5
92	6.0
93	6.0
94	6.0
95	4.0
96	2.0
97	1.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
50	363882.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	49.80201892908017
#Duplication Level	Percentage of deduplicated	Percentage of total
1	66.54172529595779	33.139122627629426
2	13.669336730442597	13.615211331949462
3	7.083456625769266	10.583113228796378
4	4.277207155564732	8.520542069001277
5	2.743108078558688	6.830616022644626
6	1.7491474869909442	5.226664575412561
7	1.1821381797819346	4.12110076044018
8	0.8093634877261912	3.2246348588996896
9	0.5843606067802645	2.6192104200251554
>10	1.3301439583342292	8.79728777695865
>50	0.018469165595907444	0.6292018740828138
>100	0.009811744222825745	0.6728924564768649
>500	0.0	0.0
>1k	0.0017314842746163077	2.0204019976829914
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CGTTTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	3782	1.0393479204797158	No Hit
CGTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1742	0.4787266201680765	No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1505	0.41359561616128304	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0024733292660807623	0.0
2	0.0	0.0	0.0	0.014015532507790987	0.0
3	0.0	0.0	0.0	0.022259963394726863	0.0
4	0.0	0.0	0.0	0.03132883737035633	0.0
5	0.0	0.0	0.0	0.050016214047410974	0.0
6	0.0	0.0	0.0	0.12064350531216163	0.0
7	0.0	0.0	0.0	0.16653750391610467	0.0
8	0.0	0.0	0.0	0.33609796582408585	0.0
9	0.0	0.0	0.0	0.4454740822574351	0.0
10	0.0	0.0	0.0	0.621630088874965	0.0
11	0.0	0.0	0.0	0.787617964065274	0.0
12	0.0	0.0	0.0	0.9063377688371506	0.0
13	0.0	0.0	0.0	0.965148042497293	0.0
14	0.0	0.0	0.0	1.001423538399811	0.0
15	0.0	0.0	0.0	1.03110348959278	0.0
16	0.0	0.0	0.0	1.0896389488900249	0.0
17	0.0	0.0	0.0	1.1457010789211888	0.0
18	0.0	0.0	0.0	1.24326017774993	0.0
19	0.0	0.0	0.0	1.2902534338054643	0.0
20	2.748143628978625E-4	0.0	0.0	1.344666677659241	0.0
21	2.748143628978625E-4	0.0	0.0	1.4059502805854645	0.0
22	2.748143628978625E-4	0.0	0.0	1.4754783143986236	0.0
23	2.748143628978625E-4	0.0	0.0	1.5524263360100252	0.0
24	2.748143628978625E-4	0.0	0.0	1.6115114240330657	0.0
25	2.748143628978625E-4	0.0	0.0	1.656855793911213	0.0
26	2.748143628978625E-4	0.0	0.0	1.7090705228618068	0.0
27	2.748143628978625E-4	0.0	0.0	1.7755755986830897	0.0
28	2.748143628978625E-4	0.0	0.0	1.8341110579803344	0.0
29	2.748143628978625E-4	0.0	0.0	1.9077613072369615	0.0
30	2.748143628978625E-4	0.0	0.0	1.9935033884610944	0.0
31	2.748143628978625E-4	0.0	0.0	2.061107721733969	0.0
32	2.748143628978625E-4	0.0	0.0	2.136681671530881	0.0
33	2.748143628978625E-4	0.0	0.0	2.2040111904408572	0.0
34	2.748143628978625E-4	0.0	0.0	2.280134768963565	0.0
35	2.748143628978625E-4	0.0	0.0	2.377968682155204	0.0
36	2.748143628978625E-4	0.0	0.0	2.4590389192100734	0.0
37	2.748143628978625E-4	0.0	0.0	2.541483228079432	0.0
38	2.748143628978625E-4	0.0	0.0	2.641790470537152	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
ATTTACG	20	7.849382E-4	44.0	1
CGCATCG	20	7.849382E-4	44.0	21
CGCATAG	20	7.849382E-4	44.0	2
GTTAGGC	30	2.5229292E-6	44.0	4
TAACGGG	45	4.783942E-10	44.0	3
GCGTTAA	20	7.849382E-4	44.0	1
TCGATAG	20	7.849382E-4	44.0	2
ACGGGGC	55	1.8189894E-12	44.0	5
TACTCGG	20	7.849382E-4	44.0	44
CATCGTT	20	7.849382E-4	44.0	36
CGTAAAG	25	4.4368247E-5	44.0	2
TAGGCGA	50	2.7284841E-11	44.0	6
GTAGTCC	20	7.849382E-4	44.0	33
CGTTTTA	1775	0.0	43.628174	1
CGTTATT	975	0.0	43.54872	1
TAAGGAT	110	0.0	42.0	5
GTTTTAT	1975	0.0	40.881012	2
CGTTAGG	60	3.6379788E-12	40.333332	3
CGGTCTA	60	3.6379788E-12	40.333332	31
GTTATTT	1070	0.0	40.299065	2
>>END_MODULE