FastQCFastQC Report
Thu 26 May 2016
SRR1545709_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1545709_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences128132
Sequences flagged as poor quality0
Sequence length51
%GC44

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT8760.6836699653482347No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT8550.6672806168638592No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTAGGTT253.8713602E-545.0000045
TAGGTTC253.8713602E-545.0000046
GCGATTG502.1827873E-1145.0000049
AGGTACC207.009812E-445.07
CGGCGCA207.009812E-445.022
CACGCCA207.009812E-445.027
ACTTCCC207.009812E-445.044
AACGCCG207.009812E-445.013
AGCGAAG207.009812E-445.02
CGTAAGG207.009812E-445.03
TAGGATA406.7338988E-945.06
GCGTCAT207.009812E-445.037
CGTCATA207.009812E-445.038
GATAGGT207.009812E-445.04
GTACGGG207.009812E-445.03
CTAGCAG207.009812E-445.02
TCACGAC302.1503765E-644.99999625
CTCACGA302.1503765E-644.99999624
AAGGTAC302.1503765E-644.9999966
CGGTCTA302.1503765E-644.99999631