##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1545709_1.fastq.gz File type Conventional base calls Encoding Illumina 1.5 Total Sequences 128132 Sequences flagged as poor quality 0 Sequence length 51 %GC 44 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.33366372178691 31.0 31.0 33.0 30.0 34.0 2 31.53551805950114 31.0 31.0 34.0 30.0 34.0 3 31.402670683357787 31.0 31.0 34.0 30.0 34.0 4 35.34654887147629 37.0 35.0 37.0 33.0 37.0 5 33.81338775637624 37.0 35.0 37.0 32.0 37.0 6 34.70210408016733 37.0 35.0 37.0 32.0 37.0 7 15.481417600599382 0.0 0.0 35.0 0.0 37.0 8 25.033957169169295 17.0 17.0 35.0 17.0 37.0 9 32.97011675459682 32.0 32.0 37.0 27.0 39.0 10 35.908804982361936 37.0 35.0 38.0 32.0 39.0 11 36.936198607685824 37.0 37.0 39.0 34.0 39.0 12 36.99161801891799 39.0 37.0 39.0 33.0 39.0 13 36.90069615708801 39.0 37.0 39.0 33.0 39.0 14 38.03216214528767 40.0 37.0 41.0 33.0 41.0 15 38.25412855492773 40.0 37.0 41.0 33.0 41.0 16 38.21775981019574 40.0 37.0 41.0 33.0 41.0 17 38.175834295882375 40.0 37.0 41.0 34.0 41.0 18 38.07176973745825 40.0 37.0 41.0 33.0 41.0 19 38.00595479661599 40.0 37.0 41.0 34.0 41.0 20 38.08586457715481 40.0 36.0 41.0 34.0 41.0 21 38.00289545156557 40.0 36.0 41.0 34.0 41.0 22 37.99417007461056 40.0 36.0 41.0 34.0 41.0 23 37.93986669996566 40.0 36.0 41.0 34.0 41.0 24 37.71653451128524 40.0 35.0 41.0 33.0 41.0 25 37.455249274185995 39.0 35.0 41.0 33.0 41.0 26 37.39846408391347 39.0 35.0 41.0 33.0 41.0 27 37.3538772515843 39.0 35.0 41.0 33.0 41.0 28 37.22692223644367 39.0 35.0 41.0 33.0 41.0 29 37.13507164486623 39.0 35.0 41.0 33.0 41.0 30 36.83403053101489 39.0 35.0 40.0 32.0 41.0 31 36.66469734336466 39.0 35.0 40.0 31.0 41.0 32 36.49954734180376 39.0 35.0 40.0 31.0 41.0 33 36.230808853369965 39.0 35.0 40.0 30.0 41.0 34 35.93470015296725 39.0 35.0 40.0 29.0 41.0 35 35.64396091530609 39.0 35.0 40.0 26.0 41.0 36 35.44424499734649 39.0 35.0 40.0 25.0 41.0 37 35.31348140979615 38.0 35.0 40.0 25.0 41.0 38 35.15620609995942 38.0 35.0 40.0 24.0 41.0 39 35.01119158367933 38.0 34.0 40.0 23.0 41.0 40 34.835630443605034 38.0 34.0 40.0 23.0 41.0 41 34.63920019979396 38.0 34.0 40.0 22.0 41.0 42 34.60113788905191 38.0 34.0 40.0 22.0 41.0 43 34.54827833796397 38.0 34.0 40.0 22.0 41.0 44 34.352886086223585 38.0 34.0 40.0 21.0 41.0 45 34.24585583616895 38.0 34.0 40.0 21.0 41.0 46 34.229864514719196 37.0 34.0 40.0 22.0 41.0 47 34.15643242905753 37.0 33.0 40.0 21.0 41.0 48 34.02168076670933 37.0 33.0 40.0 20.0 41.0 49 33.918755658227454 37.0 33.0 40.0 20.0 41.0 50 33.804248743483285 37.0 33.0 40.0 20.0 41.0 51 33.69538444728873 36.0 33.0 40.0 20.0 41.0 >>END_MODULE >>Per sequence quality scores pass #Quality Count 12 1.0 13 0.0 14 2.0 15 6.0 16 7.0 17 19.0 18 65.0 19 94.0 20 182.0 21 310.0 22 527.0 23 909.0 24 1501.0 25 2102.0 26 2480.0 27 2475.0 28 2379.0 29 2367.0 30 2623.0 31 3423.0 32 4478.0 33 7235.0 34 10114.0 35 12308.0 36 16738.0 37 26829.0 38 23532.0 39 5426.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 33.035463428339526 22.23878500296569 26.222177129834854 18.50357443885993 2 38.99572316049074 21.75334810976181 27.19461180657447 12.056316923172977 3 30.271126650641527 22.25829613211376 34.95223675584553 12.518340461399182 4 26.572597009334125 25.81634564355508 32.56719632878594 15.043861018324852 5 20.99787718914869 32.826304123872255 32.016202041644554 14.1596166453345 6 20.127680829144946 38.55321075141261 31.653295039490526 9.665813379951924 7 35.45718477819748 57.013860706146794 6.693097742952581 0.8358567727031498 8 82.15824306184247 3.6009739955670717 12.247525988824025 1.9932569537664286 9 76.82390035276121 5.5528673555396 13.977772921674521 3.645459370024662 10 41.038148159710296 25.175600162332596 20.742671619891986 13.043580058065121 11 33.69728092904192 24.11107295601411 26.68107888739737 15.510567227546593 12 30.172790559735276 21.54340836012862 32.24409203009397 16.039709050042145 13 23.212000124871228 27.787750132675676 31.938937970218213 17.061311772234884 14 19.15992882340087 30.528673555395997 33.86273530421753 16.448662316985608 15 17.997065526176133 23.279118409140573 40.9210813848219 17.80273467986139 16 21.27337433271938 19.911497518184373 39.06050010926233 19.75462803983392 17 21.23123029375956 20.027783847906846 32.78572097524428 25.955264883089313 18 22.581400430805733 22.711734773514813 34.71732276090282 19.989542034776637 19 26.710735803702434 23.733337495707552 29.88480629350982 19.6711204070802 20 27.11110417382075 23.870695844909935 29.62803983392127 19.39016014734805 21 24.98751287734524 23.901913651546842 32.34242812100022 18.7681453501077 22 23.16517341491587 21.74476321293666 31.69699996878219 23.39306340336528 23 21.97577498204976 25.29891049854837 30.314051134767272 22.411263384634594 24 21.348297068647955 24.516904442293885 35.07398620172947 19.060812287328694 25 22.349608216526708 23.371210938719443 33.623919083445195 20.655261761308648 26 19.817844098273653 26.820778572097524 30.677738582087223 22.683638747541597 27 19.72497112352886 26.20188555552087 33.09477726094965 20.978366060000624 28 19.56185808385103 25.29813005338245 35.18793119595417 19.95208066681235 29 20.183092435925452 25.040583148627977 34.27715168732245 20.499172728124123 30 22.063184840633095 25.05228982611682 33.96653451128523 18.917990821964846 31 26.032528954515655 24.62382543002529 29.975337932756847 19.368307682702213 32 26.20266600068679 24.850154528142852 29.489120594387035 19.45805887678332 33 25.359785221490338 25.08584896825149 28.614241563387754 20.940124246870415 34 21.70730184497237 26.13164549058783 30.259419973152685 21.90163269128711 35 22.17088627353042 25.71488777198514 30.638716323791094 21.47550963069335 36 26.31817188524334 26.279149626947213 27.561421034558116 19.841257453251334 37 21.57696750226329 29.586676240127368 28.304404832516468 20.531951425092874 38 22.852214903380887 28.995098804358005 26.905847095183095 21.246839197078014 39 22.235663222302 27.7955545843349 27.122030406143665 22.84675178721943 40 23.876158961071393 25.22788998844941 29.03177972715637 21.86417132332282 41 20.7465738457216 25.38788124746355 29.300252864233762 24.56529204258109 42 22.208347641494708 27.414697343364658 27.35694440108638 23.020010614054257 43 21.93753316891955 26.217494458839326 28.73599100927169 23.108981362969438 44 21.997627446695596 26.819998126931598 29.964411700433928 21.217962725938875 45 21.367808197796023 29.32990978053882 27.33977460743608 21.962507414229076 46 22.309805513064653 27.899353791402614 28.993537914026163 20.79730278150657 47 21.711204070801983 28.029688134111698 28.51122280148597 21.74788499360035 48 21.534043018137545 28.087441076389975 29.535166859176478 20.84334904629601 49 22.446383417101114 26.558548996347515 29.96909437142946 21.025973215121905 50 21.11572440920301 27.979739643492646 28.973246339712173 21.93128960759217 51 20.85895794961446 29.23001279930072 28.65404426684981 21.256984984235007 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 142.0 1 191.5 2 241.0 3 1675.5 4 3110.0 5 2149.0 6 1188.0 7 1192.0 8 1196.0 9 1200.0 10 1204.0 11 1184.0 12 1164.0 13 1112.0 14 1060.0 15 990.0 16 920.0 17 894.5 18 869.0 19 874.5 20 880.0 21 866.0 22 852.0 23 889.0 24 926.0 25 1002.5 26 1227.5 27 1376.0 28 1586.0 29 1796.0 30 2081.5 31 2367.0 32 2713.0 33 3059.0 34 3398.5 35 3738.0 36 4057.5 37 4377.0 38 4922.5 39 5468.0 40 6249.0 41 7030.0 42 7456.0 43 7882.0 44 8132.5 45 8383.0 46 8507.5 47 8632.0 48 8503.0 49 8374.0 50 7980.5 51 7587.0 52 7225.0 53 6863.0 54 6807.0 55 6751.0 56 6599.0 57 6447.0 58 6030.0 59 5613.0 60 5052.5 61 4492.0 62 4096.0 63 3700.0 64 3277.0 65 2854.0 66 2455.0 67 2056.0 68 1784.0 69 1512.0 70 1335.5 71 1159.0 72 986.5 73 814.0 74 655.0 75 360.5 76 225.0 77 167.0 78 109.0 79 90.0 80 71.0 81 53.0 82 35.0 83 28.0 84 21.0 85 13.0 86 5.0 87 5.5 88 6.0 89 3.0 90 0.0 91 1.5 92 3.0 93 1.5 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10 0.0 11 0.0 12 0.0 13 0.0 14 0.0 15 0.0 16 0.0 17 0.0 18 0.0 19 0.0 20 0.0 21 0.0 22 0.0 23 0.0 24 0.0 25 0.0 26 0.0 27 0.0 28 0.0 29 0.0 30 0.0 31 0.0 32 0.0 33 0.0 34 0.0 35 0.0 36 0.0 37 0.0 38 0.0 39 0.0 40 0.0 41 0.0 42 0.0 43 0.0 44 0.0 45 0.0 46 0.0 47 0.0 48 0.0 49 0.0 50 0.0 51 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 51 128132.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 66.29569506446477 #Duplication Level Percentage of deduplicated Percentage of total 1 74.2577637557978 49.22970062123435 2 14.834129917830152 19.66877907158243 3 5.966143196854472 11.865888302687853 4 2.507475337273091 6.649392813660912 5 1.1642690650530925 3.859301345487466 6 0.5933181079744779 2.36006618175007 7 0.2566336260683258 1.1909593231979523 8 0.14362065312080616 0.7617144819404988 9 0.07534198196501307 0.44953641557144197 >10 0.1907093918489393 2.132176193300659 >50 0.004708873872813317 0.21540286579464926 >100 0.0035316554046099877 0.266131801579621 >500 0.0023544369364066585 1.3509505822120937 >1k 0.0 0.0 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source CGTTTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 876 0.6836699653482347 No Hit CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 855 0.6672806168638592 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0031217806636905693 0.0 2 0.0 0.0 0.0 0.015608903318452846 0.0 3 0.0 0.0 0.0 0.024974245309524554 0.0 4 0.0 0.0 0.0 0.03199825180282833 0.0 5 0.0 0.0 0.0 0.053070271282739674 0.0 6 0.0 0.0 0.0 0.08819030374925858 0.0 7 0.0 0.0 0.0 0.11316454905878313 0.0 8 0.0 0.0 0.0 0.26691224674554365 0.0 9 0.0 0.0 0.0 0.34807854400149846 0.0 10 0.0 0.0 0.0 0.48621733836980613 0.0 11 0.0 0.0 0.0 0.6235756875721912 0.0 12 0.0 0.0 0.0 0.7203508881465989 0.0 13 0.0 0.0 0.0 0.7640558174382668 0.0 14 0.0 0.0 0.0 0.7921518434114819 0.0 15 0.0 0.0 0.0 0.8163456435550838 0.0 16 0.0 0.0 0.0 0.8678550245059782 0.0 17 0.0 0.0 0.0 0.9326319732775575 0.0 18 0.0 0.0 0.0 1.0184809415290481 0.0 19 0.0 0.0 0.0 1.055942309493335 0.0 20 0.0 0.0 0.0 1.1051103549464614 0.0 21 0.0 0.0 0.0 1.1698873037180408 0.0 22 0.0 0.0 0.0 1.2276402459963163 0.0 23 0.0 0.0 0.0 1.2939780850997409 0.0 24 0.0 0.0 0.0 1.3408047950550994 0.0 25 0.0 0.0 0.0 1.3751443823556957 0.0 26 0.0 0.0 0.0 1.4235319826428996 0.0 27 0.0 0.0 0.0 1.481284924921175 0.0 28 0.0 0.0 0.0 1.5304529703743015 0.0 29 0.0 0.0 0.0 1.581962351325196 0.0 30 0.0 0.0 0.0 1.651421971092311 0.0 31 0.0 0.0 0.0 1.7177598101957356 0.0 32 0.0 0.0 0.0 1.8004869977835358 0.0 33 0.0 0.0 0.0 1.869946617550651 0.0 34 0.0 0.0 0.0 1.939406237317766 0.0 35 0.0 0.0 0.0 2.0369618830580962 0.0 36 0.0 0.0 0.0 2.109543283488902 0.0 37 0.0 0.0 0.0 2.1797833484219398 0.0 38 0.0 0.0 0.0 2.2781194393281927 0.0 39 0.0 0.0 0.0 2.4115755627009645 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position TTAGGTT 25 3.8713602E-5 45.000004 5 TAGGTTC 25 3.8713602E-5 45.000004 6 GCGATTG 50 2.1827873E-11 45.000004 9 AGGTACC 20 7.009812E-4 45.0 7 CGGCGCA 20 7.009812E-4 45.0 22 CACGCCA 20 7.009812E-4 45.0 27 ACTTCCC 20 7.009812E-4 45.0 44 AACGCCG 20 7.009812E-4 45.0 13 AGCGAAG 20 7.009812E-4 45.0 2 CGTAAGG 20 7.009812E-4 45.0 3 TAGGATA 40 6.7338988E-9 45.0 6 GCGTCAT 20 7.009812E-4 45.0 37 CGTCATA 20 7.009812E-4 45.0 38 GATAGGT 20 7.009812E-4 45.0 4 GTACGGG 20 7.009812E-4 45.0 3 CTAGCAG 20 7.009812E-4 45.0 2 TCACGAC 30 2.1503765E-6 44.999996 25 CTCACGA 30 2.1503765E-6 44.999996 24 AAGGTAC 30 2.1503765E-6 44.999996 6 CGGTCTA 30 2.1503765E-6 44.999996 31 >>END_MODULE