##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1545708_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	1128518
Sequences flagged as poor quality	0
Sequence length	50
%GC	46
>>END_MODULE
>>Per base sequence quality	fail
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.498895010979002	31.0	31.0	33.0	30.0	34.0
2	31.706409645216116	31.0	31.0	34.0	30.0	34.0
3	31.74052695659263	31.0	31.0	34.0	30.0	34.0
4	35.67734231975033	37.0	35.0	37.0	35.0	37.0
5	27.128705080468368	35.0	25.0	37.0	0.0	37.0
6	31.355189726703518	35.0	26.0	37.0	17.0	37.0
7	14.952821310781042	0.0	0.0	32.0	0.0	37.0
8	25.053867993244236	17.0	17.0	35.0	17.0	37.0
9	33.121214725861705	32.0	32.0	37.0	28.0	39.0
10	35.98228650318382	37.0	35.0	39.0	32.0	39.0
11	36.98195775344301	38.0	37.0	39.0	34.0	39.0
12	37.12946271127266	39.0	37.0	39.0	34.0	39.0
13	36.80265799925212	39.0	35.0	39.0	33.0	39.0
14	38.16362610077996	40.0	37.0	41.0	33.0	41.0
15	38.38401957257217	40.0	37.0	41.0	34.0	41.0
16	38.39526086424851	40.0	37.0	41.0	34.0	41.0
17	38.408431234592626	40.0	37.0	41.0	34.0	41.0
18	38.377665221113	40.0	37.0	41.0	34.0	41.0
19	38.30298674899293	40.0	37.0	41.0	34.0	41.0
20	38.321103429453494	40.0	37.0	41.0	34.0	41.0
21	38.20800908802518	40.0	37.0	41.0	34.0	41.0
22	38.09841934289041	40.0	36.0	41.0	34.0	41.0
23	37.9809023870244	40.0	36.0	41.0	34.0	41.0
24	37.813725611820104	40.0	35.0	41.0	33.0	41.0
25	37.62682828275668	39.0	35.0	41.0	33.0	41.0
26	37.384955313074315	39.0	35.0	41.0	33.0	41.0
27	37.196612725716385	39.0	35.0	40.0	33.0	41.0
28	37.1950682222171	39.0	35.0	40.0	33.0	41.0
29	37.0793704663993	39.0	35.0	40.0	32.0	41.0
30	36.85564962189349	39.0	35.0	40.0	32.0	41.0
31	36.670925940038174	39.0	35.0	40.0	31.0	41.0
32	36.41926136756348	38.0	35.0	40.0	31.0	41.0
33	36.156678050327955	38.0	35.0	40.0	30.0	41.0
34	35.86541021055933	38.0	35.0	40.0	29.0	41.0
35	35.63274577809127	38.0	35.0	40.0	28.0	41.0
36	35.4077817101721	38.0	35.0	40.0	27.0	41.0
37	35.31258695031892	38.0	35.0	40.0	26.0	41.0
38	35.11773405475145	38.0	34.0	40.0	26.0	41.0
39	35.017355505184675	38.0	34.0	40.0	25.0	41.0
40	34.773786505842175	38.0	34.0	40.0	24.0	41.0
41	34.54734262103042	38.0	34.0	40.0	23.0	41.0
42	34.52149987860185	38.0	34.0	40.0	23.0	41.0
43	34.39451209462322	37.0	34.0	40.0	23.0	40.0
44	34.16733627642625	37.0	33.0	40.0	23.0	40.0
45	33.939996526417836	37.0	33.0	40.0	23.0	40.0
46	33.887269853028485	36.0	33.0	39.0	23.0	40.0
47	33.73075484839409	36.0	33.0	39.0	23.0	40.0
48	33.62824872974999	36.0	33.0	39.0	23.0	40.0
49	33.456219572926614	36.0	33.0	39.0	22.0	40.0
50	33.57069625827856	36.0	33.0	39.0	22.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
11	1.0
12	2.0
13	3.0
14	12.0
15	18.0
16	64.0
17	184.0
18	429.0
19	892.0
20	1608.0
21	2731.0
22	4498.0
23	7008.0
24	10799.0
25	15759.0
26	19944.0
27	21437.0
28	21386.0
29	22714.0
30	26943.0
31	34900.0
32	47744.0
33	79056.0
34	88939.0
35	115751.0
36	164134.0
37	285365.0
38	123529.0
39	32668.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	35.033380061283914	22.608943765185845	25.29600768441443	17.061668489115814
2	36.78541237268701	22.671149241748914	28.10943201614861	12.434006369415464
3	30.55086405356405	23.481149613918433	33.36836452763713	12.599621804880384
4	26.435112244554364	26.50591306474509	31.96112069103018	15.097853999670363
5	17.514031676942682	47.37842019356359	23.870155371912542	11.237392757581182
6	20.16520782123103	40.31818721544539	29.387922921920605	10.128682041402973
7	38.18317474776654	55.80805977396904	4.948259575833084	1.0605059024313304
8	84.65119741111793	3.3299424555035895	10.147290517297908	1.8715696160805588
9	80.22565878435258	4.913789589532466	11.679033918820966	3.1815177072939913
10	42.94313426990088	25.961393615343308	17.929000689399725	13.166471425356086
11	35.49788306433748	22.592727807620257	25.40517740966471	16.504211718377555
12	31.696791721532136	21.518132630582766	30.29442153337386	16.490654114511244
13	23.07619373372866	29.249245470608354	29.927214275713816	17.747346519949172
14	19.035761946198466	31.78682130014763	31.870470829884855	17.30694592376905
15	17.744954001619824	22.514394985281584	41.09194536551477	18.64870564758382
16	19.90114468710291	19.315952426102196	39.56445532991055	21.21844755688434
17	20.377964728963118	19.5907375868174	31.61207885031519	28.419218833904285
18	22.975264904946133	22.293042733921833	33.966671333554274	20.765021027577763
19	27.100941234433122	23.97516034303396	28.0095665288458	20.91433189368712
20	28.775349617817348	22.97880937654517	27.726097412712956	20.519743592924524
21	24.66039531491744	24.2913272096679	30.13217334592802	20.916104129486637
22	23.61575092289179	22.44323971793095	28.844644037578487	25.096365321598768
23	21.98901568251459	26.120629001929963	28.543009504500596	23.347345811054854
24	21.60895971530804	23.729262625850893	35.14494230486355	19.51683535397752
25	22.045106945569323	24.07821585477591	32.37830499823662	21.498372201418142
26	20.6076464885806	27.07976301662889	29.216104661157377	23.09648583363314
27	20.596924461993517	26.086867909949152	31.374776476759784	21.941431151297543
28	19.862244111303497	25.160520257541307	34.56090199713252	20.416333634022674
29	20.149169087245397	24.480779216636332	34.079828589353475	21.290223106764802
30	21.572097210678077	25.303539686562377	32.69819356004955	20.426169542709996
31	26.98893593190361	24.628672294106075	27.585470502021238	20.79692127196908
32	26.432896949804967	24.83735305949927	28.02480775672165	20.704942233974112
33	25.739243857873777	25.255069037445573	27.706602818918263	21.29908428576239
34	21.38999998227764	26.394616656535387	29.471395228077885	22.743988133109085
35	22.0580442669058	25.4493060810727	30.123312166930432	22.369337485091066
36	27.013835844886835	26.239900471237497	26.581321698014566	20.164941985861105
37	22.08409613315871	29.463951837719915	27.176615703072528	21.275336326048855
38	22.489317848718407	29.227978641014147	26.51902760966152	21.76367590060593
39	22.63641342007837	27.922106692139604	26.060638820116296	23.380841067665735
40	24.208918244990333	24.98533474875899	27.78475841767699	23.020988588573687
41	21.1212404232808	24.68059880303194	28.206196090802273	25.991964682884987
42	22.937339058836457	26.878880088753572	25.316033948948974	24.867746903461
43	22.51342025559185	26.007914805080645	27.033153215101574	24.44551172422593
44	22.45573398031755	26.430149984315715	28.488779089035354	22.625336946331384
45	20.87082350480896	29.532360139581293	26.625893428372432	22.970922927237314
46	22.690821059123557	27.677538151806175	28.298884023117044	21.332756765953224
47	22.32130989492414	27.436159635911878	27.816658662068306	22.425871807095675
48	22.645451822655907	25.96954590002109	28.900026406313412	22.484975871009592
49	22.12795896919677	25.22511825243372	30.176213405546036	22.47070937282347
50	21.22642261798217	28.14656035614851	28.897368052614137	21.729648973255188
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	597.0
1	1218.0
2	1839.0
3	11372.5
4	20906.0
5	14841.5
6	8777.0
7	8630.5
8	8484.0
9	8556.0
10	8628.0
11	8482.5
12	8337.0
13	7808.0
14	7279.0
15	6919.5
16	6560.0
17	6284.5
18	6009.0
19	5878.0
20	5747.0
21	5982.0
22	6217.0
23	6653.5
24	7090.0
25	8354.5
26	9619.0
27	10773.0
28	11927.0
29	13830.0
30	15733.0
31	18260.0
32	20787.0
33	23938.5
34	27090.0
35	29989.5
36	32889.0
37	36469.0
38	40049.0
39	47112.5
40	54176.0
41	62669.5
42	71163.0
43	74545.0
44	77927.0
45	79588.0
46	81249.0
47	82023.0
48	82797.0
49	80707.5
50	78618.0
51	74340.0
52	70062.0
53	66141.0
54	62220.0
55	60630.5
56	59041.0
57	57123.0
58	55205.0
59	51538.5
60	47872.0
61	43085.5
62	38299.0
63	33816.0
64	29333.0
65	25344.5
66	21356.0
67	18666.0
68	15976.0
69	13710.0
70	11444.0
71	9648.5
72	7853.0
73	6320.0
74	4787.0
75	3607.5
76	2428.0
77	1768.5
78	1109.0
79	837.5
80	566.0
81	413.0
82	260.0
83	189.0
84	118.0
85	85.5
86	53.0
87	38.0
88	23.0
89	18.0
90	13.0
91	9.0
92	5.0
93	3.0
94	1.0
95	0.5
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
50	1128518.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	32.160646923729956
#Duplication Level	Percentage of deduplicated	Percentage of total
1	67.14624828239268	21.594667832631398
2	8.683382901498133	5.585264231972705
3	4.829676513792361	4.659765633477213
4	3.4564442233115416	4.44645929109954
5	2.4533311906944184	3.94503591054486
6	1.9775292506252058	3.815917200642332
7	1.6114900355841975	3.627859343887268
8	1.3404483687788042	3.4487749366227907
9	1.048038049584734	3.033502350779629
>10	7.349653922820274	41.372669377853605
>50	0.07917503062824854	1.6228649461982598
>100	0.022666731825751397	1.354870044057869
>500	6.384994878816926E-4	0.10626633035710802
>1k	9.57749231822539E-4	0.7119330770011468
>5k	3.192497439408463E-4	0.6741494928741751
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CGTTTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	6566	0.5818250129816271	No Hit
CGTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	2919	0.25865781493959333	No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	2781	0.2464293879229219	No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATA	1234	0.10934694883023577	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0057597663484321915	0.0
2	0.0	0.0	0.0	0.016747628305441294	0.0
3	0.0	0.0	0.0	0.025431583723077522	0.0
4	0.0	0.0	0.0	0.03411553914071375	0.0
5	0.0	0.0	0.0	0.0554709805249008	0.0
6	0.0	0.0	0.0	0.11457504443881267	0.0
7	0.0	0.0	0.0	0.1584378804768732	0.0
8	0.0	0.0	0.0	0.388739922624185	0.0
9	0.0	0.0	0.0	0.5101380748911404	0.0
10	0.0	0.0	0.0	0.6938303155111394	0.0
11	0.0	0.0	0.0	0.8278113419546698	0.0
12	0.0	0.0	0.0	0.9539059190903468	0.0
13	0.0	0.0	0.0	1.0075160520257542	0.0
14	0.0	0.0	0.0	1.037023778087722	0.0
15	0.0	0.0	0.0	1.0652909390900278	0.0
16	0.0	0.0	0.0	1.123420273314205	0.0
17	0.0	0.0	0.0	1.1891702214763078	0.0
18	0.0	0.0	0.0	1.2903648856287626	0.0
19	0.0	0.0	0.0	1.333607439136992	0.0
20	0.0	0.0	0.0	1.382432535413702	0.0
21	0.0	0.0	0.0	1.440650481427855	0.0
22	0.0	0.0	0.0	1.5044509702104885	0.0
23	0.0	0.0	0.0	1.5834040750789975	0.0
24	0.0	0.0	0.0	1.6432170333127163	0.0
25	0.0	0.0	0.0	1.6934599182290402	0.0
26	0.0	0.0	0.0	1.7492853459138444	0.0
27	0.0	0.0	0.0	1.8090096923575876	0.0
28	0.0	0.0	0.0	1.8649237318323677	0.0
29	0.0	0.0	0.0	1.9278381027152425	0.0
30	0.0	0.0	0.0	2.0133484800419668	0.0
31	0.0	0.0	0.0	2.0859215360322123	0.0
32	0.0	0.0	0.0	2.157608474122699	0.0
33	0.0	0.0	0.0	2.2294726357931376	0.0
34	0.0	0.0	0.0	2.30727378739196	0.0
35	0.0	0.0	0.0	2.394467788728226	0.0
36	0.0	0.0	0.0	2.471116987057362	0.0
37	0.0	0.0	0.0	2.5540576224747853	0.0
38	0.0	0.0	0.0	2.6477202844792904	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TCGTACA	25	4.4427892E-5	44.0	34
CGTTTTA	3745	0.0	43.530037	1
CGTTATT	2040	0.0	41.519608	1
CGTTCGA	85	0.0	41.411762	14
TTACGGG	310	0.0	40.45161	3
GTTTTAT	4340	0.0	40.35023	2
CGAAATT	50	1.3478711E-9	39.6	37
CGGTCTA	250	0.0	39.6	31
ACGGTCT	250	0.0	39.6	30
TTGCGTA	45	2.3508619E-8	39.11111	1
TCGAATA	45	2.3508619E-8	39.11111	1
GTTATTT	2285	0.0	38.897156	2
TACGGGA	215	0.0	38.88372	4
AGGCGAT	860	0.0	38.627907	7
GCGAATA	40	4.1244493E-7	38.500004	1
TCGCTAA	40	4.1244493E-7	38.500004	12
GCGTAAA	40	4.1244493E-7	38.500004	1
ACGGGAT	235	0.0	38.382977	5
AGTACGG	115	0.0	38.260868	2
TATACGG	75	0.0	38.13333	2
>>END_MODULE