FastQCFastQC Report
Thu 26 May 2016
SRR1545707_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1545707_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences429214
Sequences flagged as poor quality0
Sequence length51
%GC46

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT17260.40213040581155324No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT16540.3853555569016854No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATAC5230.12185063860917864No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGTTAG253.8867074E-545.02
GACGTAA207.028342E-445.01
TAACGGG502.1827873E-1145.03
TGCGGGT207.028342E-445.04
TAACGCC207.028342E-445.012
CGGTCTA1000.045.031
ATAACGC207.028342E-445.011
AAGGCGC551.8189894E-1245.06
TCGACGG207.028342E-445.02
CGTTTTA9100.043.5164831
CGTTTTT14250.042.01
CGTTATT2400.041.2499961
CGTTGAT556.002665E-1140.90909225
CACGACG1100.040.90909226
GGTACCT2050.040.6097568
GTTGATC2000.040.516
GTAGCAT1950.040.38461729
ACATACG950.040.26315717
TTACACG1400.040.17857434
CGTATGG451.9233994E-840.0000042
Produced by FastQC (version 0.11.5)