##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1545707_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	429214
Sequences flagged as poor quality	0
Sequence length	51
%GC	46
>>END_MODULE
>>Per base sequence quality	fail
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	30.966771820117703	31.0	31.0	33.0	30.0	33.0
2	31.161094465697765	31.0	31.0	33.0	30.0	34.0
3	31.07513734407545	31.0	31.0	34.0	28.0	34.0
4	35.068795519251466	35.0	35.0	37.0	32.0	37.0
5	32.82246152269031	35.0	35.0	37.0	28.0	37.0
6	34.00893493688463	35.0	35.0	37.0	28.0	37.0
7	15.43155162692736	0.0	0.0	35.0	0.0	37.0
8	24.94677713215319	17.0	17.0	35.0	17.0	37.0
9	32.60486843392806	32.0	32.0	37.0	27.0	39.0
10	35.40772435195497	37.0	34.0	37.0	32.0	39.0
11	36.39382219592092	37.0	35.0	39.0	32.0	39.0
12	36.409704716062386	37.0	35.0	39.0	32.0	39.0
13	36.29457566621778	38.0	35.0	39.0	32.0	39.0
14	37.386261864710846	39.0	36.0	40.0	32.0	41.0
15	37.6339564879058	39.0	36.0	41.0	33.0	41.0
16	37.59480585442227	39.0	36.0	41.0	32.0	41.0
17	37.54189751499252	39.0	36.0	41.0	32.0	41.0
18	37.46349140521978	39.0	36.0	40.0	32.0	41.0
19	37.380134385178486	39.0	36.0	40.0	32.0	41.0
20	37.47933431807909	39.0	36.0	41.0	32.0	41.0
21	37.36882767104521	39.0	35.0	41.0	32.0	41.0
22	37.40597464202006	39.0	35.0	41.0	32.0	41.0
23	37.34961114968291	39.0	35.0	41.0	32.0	41.0
24	37.158210123621316	39.0	35.0	41.0	32.0	41.0
25	36.891795235010974	39.0	35.0	40.0	31.0	41.0
26	36.789517583303436	39.0	35.0	40.0	31.0	41.0
27	36.723287218031096	39.0	35.0	40.0	31.0	41.0
28	36.5875018056261	38.0	35.0	40.0	31.0	41.0
29	36.52092895385519	38.0	35.0	40.0	31.0	41.0
30	36.222860857287976	38.0	35.0	40.0	30.0	41.0
31	36.08062178773292	38.0	35.0	40.0	30.0	41.0
32	35.9609495496419	38.0	35.0	40.0	29.0	41.0
33	35.73163503520389	38.0	35.0	40.0	28.0	41.0
34	35.52186787942611	38.0	34.0	40.0	27.0	41.0
35	35.306283112852796	38.0	34.0	40.0	25.0	41.0
36	35.09114567558374	38.0	34.0	40.0	24.0	41.0
37	34.96031117344728	38.0	34.0	40.0	24.0	41.0
38	34.82849813845774	38.0	34.0	40.0	24.0	41.0
39	34.70153350077118	38.0	34.0	40.0	23.0	41.0
40	34.525481461462114	38.0	33.0	40.0	23.0	41.0
41	34.34332524102196	38.0	33.0	40.0	23.0	41.0
42	34.28134683398025	38.0	33.0	40.0	22.0	41.0
43	34.2006877688053	37.0	33.0	40.0	22.0	41.0
44	33.865482020623745	37.0	33.0	40.0	21.0	41.0
45	33.83107028195725	37.0	33.0	40.0	20.0	41.0
46	33.800409585894215	37.0	33.0	40.0	21.0	41.0
47	33.73567497798301	37.0	33.0	40.0	20.0	41.0
48	33.68526655700886	36.0	33.0	40.0	20.0	41.0
49	33.54292264464812	36.0	32.0	40.0	20.0	41.0
50	33.43902342421263	36.0	32.0	40.0	20.0	41.0
51	33.26450674954685	36.0	32.0	40.0	20.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
10	1.0
11	2.0
12	0.0
13	7.0
14	3.0
15	18.0
16	54.0
17	133.0
18	338.0
19	617.0
20	1016.0
21	1610.0
22	2469.0
23	3788.0
24	5249.0
25	7044.0
26	8177.0
27	8716.0
28	9170.0
29	10363.0
30	12545.0
31	15539.0
32	20083.0
33	28838.0
34	37031.0
35	42773.0
36	53700.0
37	78358.0
38	66643.0
39	14929.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	34.91055743754863	23.34616298629588	25.35891187146738	16.384367704688106
2	36.19849305940626	22.664451765319864	28.63746289729599	12.499592277977884
3	31.165106450395374	23.26368664582236	32.81603116394153	12.755175739840732
4	26.995624560242675	27.212066707982498	30.378319439720048	15.413989292054781
5	21.482290885199458	35.82129194294688	28.905394511828597	13.791022660025067
6	20.379111585363013	41.044560522256965	28.702931404847	9.873396487533025
7	37.43587115052165	56.380267186065694	5.340459537666526	0.8434021257461313
8	85.26725596089597	3.636880437264395	9.280918143397	1.8149454584426417
9	80.669782439529	5.132404814381637	10.824670211130112	3.373142534959251
10	42.65121827340208	26.822284454840712	17.25596089596332	13.270536375793892
11	35.712954377070645	23.182841193437305	24.751522550522584	16.352681878969467
12	31.989403887105265	22.18520365132545	29.493912127749795	16.331480333819492
13	23.306788688160218	30.061694166546292	28.948962522191728	17.682554623101762
14	19.102825164137236	32.67414390024557	31.04442073184938	17.178610203767818
15	17.659955173875968	23.106888405317626	40.4499853220072	18.7831710987992
16	19.811795514591786	19.90265927952024	38.998261939265724	21.287283266622246
17	20.257959898791743	20.08275592128868	31.09707511870535	28.562209061214222
18	22.894174001779998	22.646744980359447	33.6785379787239	20.780543039136653
19	27.440624024379446	24.249907971315007	27.27194359923022	21.037524405075324
20	29.258831258999006	23.265783501936095	27.168032729594096	20.3073525094708
21	24.72193358091768	25.13315036322207	29.33082331890386	20.81409273695639
22	23.43166811893368	22.887883433438798	28.224848210915766	25.455600236711756
23	22.050072923995955	26.783376124730324	27.817825140838835	23.348725810434885
24	21.409832857269333	24.317473334979752	34.97555997707437	19.29713383067654
25	22.03632686725037	24.629438927900768	32.03343786549367	21.300796339355195
26	20.68595153000601	27.788003187221292	28.47577199252587	23.05027329024682
27	20.545462170385868	26.902663939200494	30.64135838998728	21.91051550042636
28	19.859324253169746	25.9425368231232	33.91315287944941	20.284986044257643
29	20.12981869184137	25.001514396082143	33.614234391236074	21.254432520840417
30	21.66168857492999	26.06555238179556	32.00944051219206	20.263318531082398
31	27.282194895786247	25.12942261901988	26.901499019137308	20.68688346605656
32	26.808305414082483	25.094009049099053	27.523100364852965	20.574585171965502
33	26.157814050799832	25.546464001640206	27.07507210855191	21.220649839008047
34	21.345063301756234	26.585339713988827	28.98554101217574	23.084055972079195
35	22.171224610567222	25.555084410107774	29.993429850843633	22.280261128481364
36	27.189933226781974	26.74167198646829	26.0991020796153	19.969292707134436
37	21.83293182421822	30.25460492900977	26.878433601886236	21.03402964488577
38	22.456630026047613	29.633469551319386	26.127759113169652	21.782141309463345
39	22.41469290377294	28.590400126743305	25.73355016378776	23.261356805695993
40	24.24967498730237	25.145032547866563	27.664521660523654	22.94077080430741
41	21.038223357113235	24.939074680695413	27.93827787537219	26.084424086819162
42	22.81356153340758	27.223249940589078	24.995456811753577	24.96773171424977
43	22.332682531324703	26.108887408146053	26.862590689026923	24.69583937150233
44	22.128588536254643	26.84138914387695	28.605311103552072	22.424711216316336
45	20.82434403351242	30.177021252801634	26.344434244922116	22.654200468763833
46	22.62134972298201	28.202248761689972	27.904961161565094	21.271440353762923
47	22.046578163806398	28.02541389609845	27.31178386539116	22.616224074703993
48	22.326158978970863	26.450441970671974	28.92263532876374	22.300763721593427
49	21.975751023964733	25.782942774466814	30.227578783543873	22.013727418024576
50	21.188731029276774	28.957815914671936	28.37698677116776	21.47646628488353
51	21.32246385253044	29.12300157963161	27.457398873289314	22.09713569454864
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	253.0
1	477.0
2	701.0
3	3600.5
4	6500.0
5	4692.0
6	2884.0
7	2838.0
8	2792.0
9	2846.0
10	2900.0
11	2781.0
12	2662.0
13	2626.5
14	2591.0
15	2403.0
16	2215.0
17	2156.0
18	2097.0
19	2124.5
20	2152.0
21	2223.5
22	2295.0
23	2440.0
24	2585.0
25	3053.5
26	3926.5
27	4331.0
28	4912.5
29	5494.0
30	6645.5
31	7797.0
32	8951.5
33	10106.0
34	11311.5
35	12517.0
36	13590.5
37	14664.0
38	16630.0
39	18596.0
40	21972.5
41	25349.0
42	27344.0
43	29339.0
44	29721.5
45	30104.0
46	30130.5
47	30157.0
48	29549.0
49	28941.0
50	28276.5
51	27612.0
52	26633.0
53	25654.0
54	24411.0
55	23168.0
56	22204.5
57	21241.0
58	20168.0
59	19095.0
60	17517.0
61	15939.0
62	14051.0
63	12163.0
64	10779.0
65	9395.0
66	8152.0
67	6909.0
68	6043.5
69	5178.0
70	4473.0
71	3768.0
72	3185.5
73	2603.0
74	2048.0
75	1091.0
76	689.0
77	530.5
78	372.0
79	292.0
80	212.0
81	154.0
82	96.0
83	72.0
84	48.0
85	33.0
86	18.0
87	12.5
88	7.0
89	8.0
90	9.0
91	5.0
92	1.0
93	0.5
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	429214.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	45.84388425094084
#Duplication Level	Percentage of deduplicated	Percentage of total
1	67.62924494115227	31.003872770607106
2	11.233457539212687	10.299706543310503
3	6.263965786748956	8.614935674387182
4	4.019317728562185	7.37044546863837
5	2.8027185387100975	6.424375213829587
6	1.9872854280918961	5.466292988341578
7	1.4177524058775879	4.549669403408152
8	1.1096054193402993	4.0694897926762685
9	0.8224328820875442	3.3933166065530145
>10	2.6864809204905167	16.737671406150955
>50	0.01613871111333052	0.5005623459919217
>100	0.010086694445749705	0.6672623235675393
>500	5.043347222874853E-4	0.12092106556681324
>1k	0.0010086694445749706	0.7814783969709919
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CGTTTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1726	0.40213040581155324	No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1654	0.3853555569016854	No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATAC	523	0.12185063860917864	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.005125648278015163	0.0
2	0.0	0.0	0.0	0.017473800947778964	0.0
3	0.0	0.0	0.0	0.026560177440624024	0.0
4	0.0	0.0	0.0	0.03634550597138025	0.0
5	0.0	0.0	0.0	0.06150777933618195	0.0
6	0.0	0.0	0.0	0.09878522135811041	0.0
7	0.0	0.0	0.0	0.12418047873554916	0.0
8	0.0	0.0	0.0	0.30730591266827273	0.0
9	0.0	0.0	0.0	0.39863564562199744	0.0
10	0.0	0.0	0.0	0.5524050939624523	0.0
11	0.0	0.0	0.0	0.6570149156364891	0.0
12	0.0	0.0	0.0	0.7625566733610739	0.0
13	0.0	0.0	0.0	0.8173079163307814	0.0
14	0.0	0.0	0.0	0.8380434934554791	0.0
15	0.0	0.0	0.0	0.8627397987950067	0.0
16	0.0	0.0	0.0	0.9091036173097802	0.0
17	0.0	0.0	0.0	0.9659517163932211	0.0
18	0.0	0.0	0.0	1.0610091935491386	0.0
19	0.0	0.0	0.0	1.097587683533156	0.0
20	0.0	0.0	0.0	1.1381269017320033	0.0
21	0.0	0.0	0.0	1.1833258001835913	0.0
22	0.0	0.0	0.0	1.2422707553807657	0.0
23	0.0	0.0	0.0	1.3091371670076	0.0
24	0.0	0.0	0.0	1.3585297776866552	0.0
25	0.0	0.0	0.0	1.3993019798981394	0.0
26	0.0	0.0	0.0	1.4403071661222606	0.0
27	0.0	0.0	0.0	1.4876029206875825	0.0
28	0.0	0.0	0.0	1.53839343544246	0.0
29	0.0	0.0	0.0	1.5929116943995303	0.0
30	0.0	0.0	0.0	1.6653697223296537	0.0
31	0.0	0.0	0.0	1.7306052458680286	0.0
32	0.0	0.0	0.0	1.7932779452673957	0.0
33	0.0	0.0	0.0	1.8634061330711487	0.0
34	0.0	0.0	0.0	1.934932224950724	0.0
35	0.0	0.0	0.0	2.005759364792388	0.0
36	0.0	0.0	0.0	2.0775184406846003	0.0
37	0.0	0.0	0.0	2.156733004981198	0.0
38	0.0	0.0	0.0	2.243403057682182	0.0
39	0.0	0.0	0.0	2.368515472468279	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TCGTTAG	25	3.8867074E-5	45.0	2
GACGTAA	20	7.028342E-4	45.0	1
TAACGGG	50	2.1827873E-11	45.0	3
TGCGGGT	20	7.028342E-4	45.0	4
TAACGCC	20	7.028342E-4	45.0	12
CGGTCTA	100	0.0	45.0	31
ATAACGC	20	7.028342E-4	45.0	11
AAGGCGC	55	1.8189894E-12	45.0	6
TCGACGG	20	7.028342E-4	45.0	2
CGTTTTA	910	0.0	43.516483	1
CGTTTTT	1425	0.0	42.0	1
CGTTATT	240	0.0	41.249996	1
CGTTGAT	55	6.002665E-11	40.909092	25
CACGACG	110	0.0	40.909092	26
GGTACCT	205	0.0	40.609756	8
GTTGATC	200	0.0	40.5	16
GTAGCAT	195	0.0	40.384617	29
ACATACG	95	0.0	40.263157	17
TTACACG	140	0.0	40.178574	34
CGTATGG	45	1.9233994E-8	40.000004	2
>>END_MODULE