FastQCFastQC Report
Thu 26 May 2016
SRR1545706_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1545706_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences1580007
Sequences flagged as poor quality0
Sequence length50
%GC41

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT87670.5548709594324582No Hit
CGTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT36130.2286698729815754No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATA17010.10765775088338217No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGCGAC207.857379E-444.022
CGTTTTA56050.043.4897421
CGTTATT31250.042.87361
AGGCGAT11750.041.7531937
CGACGGT1650.041.33333228
TGCGATA650.040.6153831
TTGCGAA650.040.6153831
CGTTTTT27600.040.4927521
GACGGTC1650.040.029
GACCGAT5400.039.5185179
ATGGTCG452.3519533E-839.1111144
GTTTTAT65950.039.0962872
CGTTCGA404.12585E-738.514
TAGGCGA3400.038.176476
CGACCCG3400.037.5294132
GGACCGA6000.037.48
AACGGGA6150.037.203254
AGTACGG1600.037.1252
ACGGGAC3500.037.0857125
GTTATTT38450.037.019512
Produced by FastQC (version 0.11.5)