##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1545706_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	1580007
Sequences flagged as poor quality	0
Sequence length	50
%GC	41
>>END_MODULE
>>Per base sequence quality	fail
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.555258299488546	31.0	31.0	33.0	30.0	34.0
2	31.74127899433357	31.0	31.0	34.0	30.0	34.0
3	31.74324290968331	31.0	31.0	34.0	30.0	34.0
4	35.65376292636678	37.0	35.0	37.0	33.0	37.0
5	27.120964653954065	35.0	25.0	37.0	0.0	37.0
6	31.35752120085544	35.0	26.0	37.0	17.0	37.0
7	14.997184822598888	0.0	0.0	32.0	0.0	37.0
8	25.111942542026714	17.0	17.0	35.0	17.0	37.0
9	33.21619651052179	32.0	32.0	37.0	28.0	39.0
10	36.031425177230226	37.0	35.0	39.0	32.0	39.0
11	36.968617860553785	39.0	37.0	39.0	34.0	39.0
12	37.179430850622815	39.0	37.0	39.0	34.0	39.0
13	36.91881111919125	39.0	37.0	39.0	33.0	39.0
14	38.318762511811656	40.0	38.0	41.0	34.0	41.0
15	38.522116041258045	40.0	38.0	41.0	34.0	41.0
16	38.514054051659265	40.0	38.0	41.0	34.0	41.0
17	38.50957052721918	40.0	38.0	41.0	34.0	41.0
18	38.45859037333379	40.0	37.0	41.0	34.0	41.0
19	38.422782937037624	40.0	37.0	41.0	34.0	41.0
20	38.44651511037609	40.0	37.0	41.0	34.0	41.0
21	38.34156684115956	40.0	37.0	41.0	34.0	41.0
22	38.21588954985643	40.0	37.0	41.0	34.0	41.0
23	38.08425025965075	40.0	36.0	41.0	34.0	41.0
24	37.93578762625735	40.0	35.0	41.0	34.0	41.0
25	37.76715609487806	40.0	35.0	41.0	33.0	41.0
26	37.51426291149343	39.0	35.0	41.0	33.0	41.0
27	37.33086182529571	39.0	35.0	40.0	33.0	41.0
28	37.33066815526767	39.0	35.0	40.0	33.0	41.0
29	37.275077262315925	39.0	35.0	40.0	33.0	41.0
30	37.02794291417696	39.0	35.0	40.0	32.0	41.0
31	36.83957412846905	39.0	35.0	40.0	32.0	41.0
32	36.58932017389797	39.0	35.0	40.0	31.0	41.0
33	36.322547938078756	39.0	35.0	40.0	30.0	41.0
34	36.03418972194427	39.0	35.0	40.0	30.0	41.0
35	35.797183809945146	38.0	35.0	40.0	29.0	41.0
36	35.62936746482769	38.0	35.0	40.0	27.0	41.0
37	35.495448437886665	38.0	35.0	40.0	27.0	41.0
38	35.341588992960155	38.0	35.0	40.0	27.0	41.0
39	35.25455773297207	38.0	35.0	40.0	26.0	41.0
40	35.06918007325284	38.0	34.0	40.0	25.0	41.0
41	34.854086722400595	38.0	34.0	40.0	24.0	41.0
42	34.86297086025568	38.0	34.0	40.0	24.0	41.0
43	34.75441944244551	38.0	34.0	40.0	24.0	41.0
44	34.54411151343	38.0	34.0	40.0	23.0	40.0
45	34.387685624177614	38.0	34.0	40.0	23.0	40.0
46	34.28269621590284	37.0	34.0	40.0	23.0	40.0
47	34.12179249838766	37.0	33.0	40.0	23.0	40.0
48	34.01349930728155	37.0	33.0	39.0	23.0	40.0
49	33.86795817993212	37.0	33.0	39.0	23.0	40.0
50	34.01976889975804	37.0	33.0	39.0	23.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
9	1.0
10	0.0
11	3.0
12	3.0
13	6.0
14	13.0
15	22.0
16	81.0
17	202.0
18	574.0
19	1161.0
20	2247.0
21	3805.0
22	6195.0
23	9926.0
24	15204.0
25	22482.0
26	27744.0
27	28183.0
28	27644.0
29	28958.0
30	33511.0
31	43946.0
32	60106.0
33	100612.0
34	115030.0
35	153051.0
36	224960.0
37	439879.0
38	182315.0
39	52143.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	34.292379717305046	21.892308072052845	26.056023802426193	17.759288408215912
2	36.788001572144935	23.144581005020864	27.704054475708016	12.363362947126182
3	29.355819309661285	23.184390955229944	35.06009783500959	12.399691900099178
4	26.021846738653693	27.011082862291115	32.80928502215497	14.157785376900229
5	16.895558057654174	47.885800505947124	24.653181916282648	10.56545952011605
6	19.994341797219885	39.81298817030557	30.529168541658358	9.66350149081618
7	38.42622216230688	55.62988012078427	5.10168625835202	0.8422114585568291
8	85.61708903821312	2.783975007705662	10.125145015180312	1.4737909389009036
9	80.5043901704233	5.25890075170553	11.624695333628269	2.6120137442429052
10	39.1135608892872	33.817191949149596	17.133784850320282	9.935462311242924
11	30.803660996438627	24.413056397851403	31.1761909915589	13.607091614151075
12	29.040820705224725	24.105715987334232	32.0958704613334	14.757592846107645
13	20.857692402628597	31.089039478939018	32.4232740741022	15.629994044330184
14	16.767710522801483	34.114975439982224	33.23801729992336	15.879296737292936
15	16.366573059486445	26.196592799905318	42.15974992515856	15.27708421544968
16	18.788904099791964	24.07464017564479	40.668427418359535	16.468028306203706
17	19.38466095403375	22.74154481594069	33.497699693735534	24.37609453629003
18	20.90016056890887	24.68267545650114	36.1251564075349	18.292007567055084
19	24.41008172748602	25.343558604487193	31.312582792354714	18.93377687567207
20	25.74937959135624	25.603684034311243	31.186570692408328	17.460365681924195
21	22.488318089729983	26.739818241311593	33.02846126631084	17.743402402647586
22	22.061357956009058	24.848244343221264	32.00992147503144	21.08047622573824
23	19.533521054020646	28.603354288936693	32.02625051661164	19.836874140431025
24	18.853777230100878	25.7114683669123	37.818440044885875	17.616314358100947
25	18.69865133508902	27.12703171568227	35.40199505445229	18.772321894776415
26	18.157008165153698	31.261886814425505	31.807707181044137	18.77339783937666
27	18.47428524050843	29.745058091514785	33.74447075234477	18.036185915632018
28	17.147582257546958	27.816205877568894	37.72806069846526	17.308151166418885
29	18.26726084124944	25.74229101516639	37.119139345585175	18.871308797998996
30	19.50301486006075	27.97993932938272	34.175038465019455	18.342007345537077
31	23.73717331632075	27.023297998046846	30.809167301157526	18.430361384474878
32	24.274639289572768	27.29576514534429	31.147203778211107	17.28239178687183
33	23.30628914935187	27.73816824862168	30.2638532614096	18.691689340616847
34	20.284213930697774	27.857091772378222	32.224287613915635	19.634406683008365
35	20.123581730967015	27.898294121481744	32.19004725928429	19.78807688826695
36	24.156285383545768	27.993420282315206	29.66138757613099	18.188906758008034
37	20.852819006498073	30.8256229244554	30.319106181175147	18.00245188787138
38	20.746490363650288	30.910306093580598	28.729999297471466	19.61320424529765
39	20.55687095057174	30.309865715784806	29.362274977262757	19.770988356380702
40	22.028320127695636	28.18468525772354	29.30607269461464	20.48092191996618
41	18.95846031061888	27.732788525620457	29.998474690302007	23.310276473458664
42	21.137881034704275	28.61740485959872	28.517152139199382	21.727561966497618
43	21.205855417096252	29.329490312384692	28.43993729141706	21.02471697910199
44	20.797059759861824	30.093094524264767	29.381768561784856	19.72807715408856
45	19.13864938573057	32.21719903772578	28.205507950281234	20.438643626262415
46	20.784654751529583	31.201254171658732	29.268224761029536	18.745866315782145
47	21.033577699339308	28.891327696649444	29.76170358738917	20.313391016622077
48	21.41275323463757	27.28715758854233	31.021887877711933	20.27820129910817
49	20.434656302155624	27.573042397913426	31.272772842145635	20.719528457785312
50	19.143649363578767	30.822774835807685	29.69271655125579	20.340859249357756
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	833.0
1	1655.5
2	2478.0
3	15428.0
4	28378.0
5	20842.0
6	13306.0
7	13215.0
8	13124.0
9	13182.5
10	13241.0
11	13145.0
12	13049.0
13	12547.5
14	12046.0
15	11639.5
16	11233.0
17	10778.5
18	10324.0
19	10472.5
20	10621.0
21	11225.5
22	11830.0
23	13339.0
24	14848.0
25	18019.5
26	21191.0
27	24965.5
28	28740.0
29	33528.0
30	38316.0
31	44933.5
32	51551.0
33	57157.0
34	62763.0
35	67070.0
36	71377.0
37	77678.5
38	83980.0
39	94350.5
40	104721.0
41	115225.0
42	125729.0
43	130726.5
44	135724.0
45	134177.0
46	132630.0
47	125138.5
48	117647.0
49	111043.5
50	104440.0
51	95605.5
52	86771.0
53	77022.5
54	67274.0
55	59694.0
56	52114.0
57	47530.5
58	42947.0
59	37973.5
60	33000.0
61	27680.5
62	22361.0
63	18372.5
64	14384.0
65	11788.0
66	9192.0
67	7623.5
68	6055.0
69	5408.0
70	4761.0
71	3937.0
72	3113.0
73	2553.5
74	1994.0
75	1479.0
76	964.0
77	739.5
78	515.0
79	388.0
80	261.0
81	189.5
82	118.0
83	77.0
84	36.0
85	28.5
86	21.0
87	12.0
88	3.0
89	2.5
90	2.0
91	1.0
92	0.0
93	0.5
94	1.0
95	0.5
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
50	1580007.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	28.313600241226766
#Duplication Level	Percentage of deduplicated	Percentage of total
1	70.0114122813701	19.822751396584273
2	7.321022084107769	4.145689852932404
3	3.7868678628795913	3.2165958850776453
4	2.6035414573716418	2.948625281419264
5	1.959707194846014	2.774318305236297
6	1.5798911034959444	2.683944307743288
7	1.358279865166207	2.692045518261636
8	1.1249291381687871	2.5480635134255034
9	0.9742938256488403	2.4827189307225077
>10	9.001388873226665	50.342430953572645
>50	0.2637191205473358	4.618279922901683
>100	0.013819103133712362	0.5515289689693609
>500	2.820225093173298E-4	0.04862909205993663
>1k	5.640450186346596E-4	0.4243267573177393
>5k	2.820225093173298E-4	0.7000513137758037
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CGTTTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	8767	0.5548709594324582	No Hit
CGTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	3613	0.2286698729815754	No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATA	1701	0.10765775088338217	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.004367069259819735	0.0
2	0.0	0.0	0.0	0.017974603909982678	0.0
3	0.0	0.0	0.0	0.02531634353518687	0.0
4	0.0	0.0	0.0	0.037721351867428435	0.0
5	0.0	0.0	0.0	0.07360726882855582	0.0
6	0.0	0.0	0.0	0.13537914705441179	0.0
7	0.0	0.0	0.0	0.1829738729005631	0.0
8	0.0	0.0	0.0	0.4494283886084049	0.0
9	0.0	0.0	0.0	0.6048707379144522	0.0
10	0.0	0.0	0.0	0.8595531538784322	0.0
11	0.0	0.0	0.0	1.0053120017822705	0.0
12	0.0	0.0	0.0	1.1613872596766976	0.0
13	0.0	0.0	0.0	1.2306907501042716	0.0
14	0.0	0.0	0.0	1.2598045451697366	0.0
15	0.0	0.0	0.0	1.293981608942239	0.0
16	0.0	0.0	0.0	1.3619559913342156	0.0
17	0.0	0.0	0.0	1.438284767092804	0.0
18	0.0	0.0	0.0	1.550056423800654	0.0
19	0.0	0.0	0.0	1.6025245457773287	0.0
20	0.0	0.0	0.0	1.664043260567833	0.0
21	0.0	0.0	0.0	1.7318277703832958	0.0
22	0.0	0.0	0.0	1.8072704741181527	0.0
23	0.0	0.0	0.0	1.8879030282777227	0.0
24	0.0	0.0	0.0	1.9535989397515328	0.0
25	0.0	0.0	0.0	2.0097379315408097	0.0
26	0.0	0.0	0.0	2.064864269588679	0.0
27	0.0	0.0	0.0	2.126193111802669	0.0
28	0.0	0.0	0.0	2.188787771193419	0.0
29	0.0	0.0	0.0	2.2552431729732842	0.0
30	0.0	0.0	0.0	2.3475212451590406	0.0
31	0.0	0.0	0.0	2.422141167729004	0.0
32	0.0	0.0	0.0	2.4962547634282632	0.0
33	0.0	0.0	0.0	2.573532902069421	0.0
34	0.0	0.0	0.0	2.6543553288055053	0.0
35	0.0	0.0	0.0	2.747203018720803	0.0
36	0.0	0.0	0.0	2.8311266975399474	0.0
37	0.0	0.0	0.0	2.9150503763590923	0.0
38	0.0	0.0	0.0	2.997961401436829	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TCGCGAC	20	7.857379E-4	44.0	22
CGTTTTA	5605	0.0	43.489742	1
CGTTATT	3125	0.0	42.8736	1
AGGCGAT	1175	0.0	41.753193	7
CGACGGT	165	0.0	41.333332	28
TGCGATA	65	0.0	40.615383	1
TTGCGAA	65	0.0	40.615383	1
CGTTTTT	2760	0.0	40.492752	1
GACGGTC	165	0.0	40.0	29
GACCGAT	540	0.0	39.518517	9
ATGGTCG	45	2.3519533E-8	39.11111	44
GTTTTAT	6595	0.0	39.096287	2
CGTTCGA	40	4.12585E-7	38.5	14
TAGGCGA	340	0.0	38.17647	6
CGACCCG	340	0.0	37.52941	32
GGACCGA	600	0.0	37.4	8
AACGGGA	615	0.0	37.20325	4
AGTACGG	160	0.0	37.125	2
ACGGGAC	350	0.0	37.085712	5
GTTATTT	3845	0.0	37.01951	2
>>END_MODULE