FastQCFastQC Report
Thu 26 May 2016
SRR1545705_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1545705_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences612396
Sequences flagged as poor quality0
Sequence length51
%GC42

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT24260.39614889711885776No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT21580.3523863643786047No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATAC6730.10989621094847125No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGTTAA406.8066583E-945.01
GCGACTA207.030702E-445.01
GTGCGTA207.030702E-445.01
ATCGAAG351.210683E-745.02
ACGTTCG253.8886647E-544.99999613
CGTTCGA253.8886647E-544.99999614
TCGATAG302.1638334E-644.9999962
TCGTCGC253.8886647E-544.99999626
CGGGAGT253.8886647E-544.9999966
CCGCAAC253.8886647E-544.99999629
CGTTTTA15150.044.2574231
CGTTTTT21000.042.751
CGTTAAG900.042.52
CGTTATT3300.041.5909081
TCACGAC603.6379788E-1241.24999625
AGGCGAT4750.041.2105267
CGACGGT556.002665E-1140.90909228
CACGACG556.002665E-1140.90909226
GACGGTC556.002665E-1140.90909229
CGGTCTA556.002665E-1140.90909231
Produced by FastQC (version 0.11.5)