FastQCFastQC Report
Thu 26 May 2016
SRR1545704_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1545704_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences733936
Sequences flagged as poor quality0
Sequence length50
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT50040.6818033180004796No Hit
CGTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT21450.29225981556974995No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT20640.2812234309258573No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATA8310.11322513134660243No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGTTAG254.4412634E-544.02
GACGTAA207.854619E-444.01
ATTAACG254.4412634E-544.01
GTTCGAT351.4451325E-744.039
CGTAAAT207.854619E-444.016
CGACTTA302.5264617E-644.017
GATTACG207.854619E-444.01
AGTACGG551.8189894E-1244.02
TAGTCCA254.4412634E-544.029
CGTTTTA28650.043.0785331
CGTTATT16850.042.8249241
AGGCGAT4700.042.595747
TAACGGG1850.041.6216243
GTTTTAT31750.041.366932
GTTATTT19200.040.6770822
ACGGGAT1300.040.6153875
ACTAACC1850.040.43243414
GTTGATC5250.040.2285716
ACGGTCT1750.040.2285730
AGGTACC3150.039.111117
Produced by FastQC (version 0.11.5)