##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1545704_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	733936
Sequences flagged as poor quality	0
Sequence length	50
%GC	45
>>END_MODULE
>>Per base sequence quality	fail
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.504924135074447	31.0	31.0	33.0	30.0	34.0
2	31.70719654029779	31.0	31.0	34.0	30.0	34.0
3	31.726172309302175	31.0	31.0	34.0	30.0	34.0
4	35.64608494473632	37.0	35.0	37.0	33.0	37.0
5	26.995173966122387	35.0	22.0	37.0	0.0	37.0
6	31.28202050315014	35.0	25.0	37.0	17.0	37.0
7	15.113954077739749	0.0	0.0	32.0	0.0	37.0
8	25.151270683002334	17.0	17.0	35.0	17.0	37.0
9	33.201324093653945	32.0	32.0	37.0	28.0	39.0
10	36.05142410237405	37.0	35.0	39.0	32.0	39.0
11	37.009526716225935	39.0	37.0	39.0	34.0	39.0
12	37.05285065727802	39.0	35.0	39.0	34.0	39.0
13	36.613956802773	39.0	35.0	39.0	33.0	39.0
14	37.921559918031	40.0	36.0	41.0	33.0	41.0
15	38.19450469795731	40.0	37.0	41.0	33.0	41.0
16	38.232167382442064	40.0	37.0	41.0	34.0	41.0
17	38.25653190468924	40.0	37.0	41.0	34.0	41.0
18	38.20539938087245	40.0	36.0	41.0	34.0	41.0
19	38.137412526432826	40.0	36.0	41.0	34.0	41.0
20	38.13711413529245	40.0	36.0	41.0	34.0	41.0
21	37.99531975540101	40.0	36.0	41.0	34.0	41.0
22	37.889200148241805	40.0	35.0	41.0	34.0	41.0
23	37.762742255455514	39.0	35.0	41.0	33.0	41.0
24	37.57988162455582	39.0	35.0	41.0	33.0	41.0
25	37.40913921649844	39.0	35.0	41.0	33.0	41.0
26	37.15370413769048	39.0	35.0	40.0	33.0	41.0
27	36.94076050227813	39.0	35.0	40.0	32.0	41.0
28	36.946939515162086	39.0	35.0	40.0	32.0	41.0
29	36.850070033354406	38.0	35.0	40.0	32.0	41.0
30	36.606685051557626	38.0	35.0	40.0	31.0	41.0
31	36.37287856161845	38.0	35.0	40.0	31.0	41.0
32	36.07474766192148	38.0	35.0	40.0	30.0	41.0
33	35.79083326066578	38.0	35.0	40.0	29.0	41.0
34	35.465798107736916	38.0	35.0	40.0	27.0	41.0
35	35.20569777201282	38.0	34.0	40.0	26.0	41.0
36	34.984689400710685	38.0	34.0	40.0	25.0	41.0
37	34.84822246081402	38.0	34.0	40.0	24.0	41.0
38	34.676015347387235	38.0	34.0	40.0	24.0	41.0
39	34.58359175731944	38.0	34.0	40.0	23.0	41.0
40	34.33348820605612	37.0	34.0	40.0	23.0	41.0
41	34.119248817335574	37.0	33.0	40.0	22.0	40.0
42	34.11002049225001	37.0	33.0	40.0	22.0	40.0
43	33.987586111050554	37.0	33.0	40.0	22.0	40.0
44	33.75199199930239	37.0	33.0	40.0	21.0	40.0
45	33.52355246234004	36.0	33.0	39.0	20.0	40.0
46	33.48254343702993	36.0	33.0	39.0	20.0	40.0
47	33.34811073445096	36.0	33.0	39.0	20.0	40.0
48	33.244990026378325	36.0	33.0	39.0	20.0	40.0
49	33.1106322622136	35.0	32.0	39.0	20.0	40.0
50	33.177706230516016	35.0	33.0	39.0	20.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
11	1.0
12	2.0
13	1.0
14	7.0
15	10.0
16	46.0
17	134.0
18	306.0
19	648.0
20	1167.0
21	2149.0
22	3459.0
23	5224.0
24	8116.0
25	11812.0
26	14700.0
27	15304.0
28	15154.0
29	16035.0
30	18643.0
31	24092.0
32	32974.0
33	56792.0
34	60378.0
35	75179.0
36	100293.0
37	175779.0
38	76332.0
39	19199.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	33.34868435394912	22.362304070109655	26.62684484750714	17.662166728434087
2	36.47211745983301	22.913305792330664	28.330535632534716	12.284041115301607
3	29.91105491486996	22.795720607791413	34.68136186261472	12.611862614723899
4	25.745296592618427	25.932642628240067	33.53970918445205	14.782351594689455
5	17.025053955658258	47.01009352314098	25.10627629657082	10.85857622462994
6	19.790962699744938	39.428233524449	30.754316452660724	10.026487323145343
7	37.88654596586078	55.41900111181356	5.615476008807308	1.078976913518345
8	83.75089926097098	3.217446752850385	11.24771097207386	1.7839430141047723
9	79.6523130082187	4.535136578666259	12.794031087179263	3.0185193259357765
10	48.09738178805782	21.36099060408537	18.679421638943992	11.86220596891282
11	40.10635804756818	21.401457347779644	24.184261297987835	14.30792330666434
12	35.43238102504851	21.017500163501996	28.599087658869436	14.951031152580061
13	22.185994419131912	32.83651435547514	28.95933705391206	16.01815417148089
14	17.673748119727062	34.72305487126943	32.33592574829413	15.26727126070938
15	16.267630965097773	22.21201848662554	45.32561422249351	16.194736325783175
16	18.902874365067255	18.573009090710908	43.18578186653877	19.338334677683065
17	19.54053214449216	17.9642366636873	32.02336443504611	30.471866756774435
18	22.46313030018966	22.06200540646596	35.80543807634453	19.669426216999845
19	28.222896819341198	23.052009984521813	28.54513200061041	20.179961195526587
20	29.133194174968935	22.912488282357042	28.036777048679994	19.917540493994025
21	23.359802489590372	25.032836650607138	31.931394563013672	19.675966296788822
22	23.74266966056986	21.39055721479802	29.952611671862396	24.914161452769722
23	20.909043840334853	26.417426042597718	29.040270541300607	23.633259575766825
24	20.668423404765537	23.1873078851562	37.804522465174074	18.339746244904187
25	20.983982254583506	22.801443177606767	35.09025310108783	21.12432146672189
26	18.55352510300626	28.95538575570622	30.30127967561204	22.18980946567548
27	18.732559787229405	28.774716051535833	31.71271064506987	20.780013516164896
28	17.73151882452966	25.74965664581108	37.80206993525321	18.71675459440605
29	19.01010442327396	23.822649386322514	36.45181596215474	20.715430228248785
30	20.898143707353228	26.228444987028844	33.602248697434106	19.27116260818382
31	27.85433607290009	23.990647685901767	28.467741056440886	19.687275184757254
32	28.40819908002878	24.353758365852062	28.91042270715703	18.327619846962133
33	26.982052931045757	24.888137385276103	27.64505351965294	20.4847561640252
34	21.10211244577184	26.13593010834732	30.09894595714068	22.66301148874016
35	21.441515336487104	25.358205620108563	31.24018988031654	21.96008916308779
36	28.56584225327549	24.979834753983997	27.12266464650869	19.331658346231826
37	21.41385624904624	30.05561792853873	28.494718885570403	20.03580693684463
38	22.501825772274422	30.45647031893789	25.34158291731159	21.700120991476098
39	21.807351049682804	28.239519522138167	26.810784591572016	23.142344836607005
40	24.072671186588476	24.505678969283426	27.941128381766255	23.480521462361843
41	20.11442414597458	23.78150138431689	29.088367378082015	27.015707091626517
42	23.22804713217501	25.311062544963047	25.65795927710318	25.802931045758758
43	22.137080072376882	25.87759695668287	27.055628828671708	24.929694142268534
44	21.531986440234572	27.731028318545487	28.817362821826425	21.919622419393516
45	19.375940136469666	32.24886093610342	26.043823984652615	22.3313749427743
46	22.226597414488456	28.562027206731923	28.085827647097293	21.125547731682325
47	21.898775915066164	27.607993067515423	27.970286237492097	22.522944779926316
48	22.901588149375424	25.481513374463166	29.859824289856334	21.757074186305072
49	22.08053563253472	24.885139848706153	31.05924767282161	21.975076845937522
50	20.595528765450936	29.537997863573935	28.219218024459895	21.647255346515227
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	572.0
1	917.0
2	1262.0
3	8143.0
4	15024.0
5	10713.0
6	6402.0
7	6434.0
8	6466.0
9	6583.5
10	6701.0
11	6451.5
12	6202.0
13	6004.0
14	5806.0
15	5521.0
16	5236.0
17	4802.5
18	4369.0
19	4145.5
20	3922.0
21	3951.0
22	3980.0
23	4335.5
24	4691.0
25	5129.5
26	5568.0
27	6503.0
28	7438.0
29	8488.0
30	9538.0
31	10788.5
32	12039.0
33	13926.0
34	15813.0
35	17557.0
36	19301.0
37	21958.5
38	24616.0
39	31902.5
40	39189.0
41	46928.5
42	54668.0
43	56659.0
44	58650.0
45	57834.5
46	57019.0
47	56075.0
48	55131.0
49	53326.0
50	51521.0
51	47647.0
52	43773.0
53	40143.5
54	36514.0
55	35409.5
56	34305.0
57	33230.5
58	32156.0
59	29884.5
60	27613.0
61	24859.5
62	22106.0
63	19688.5
64	17271.0
65	14970.0
66	12669.0
67	11034.5
68	9400.0
69	8278.5
70	7157.0
71	5761.0
72	4365.0
73	3488.5
74	2612.0
75	2013.0
76	1414.0
77	1061.0
78	708.0
79	550.0
80	392.0
81	293.5
82	195.0
83	141.0
84	87.0
85	65.0
86	43.0
87	30.5
88	18.0
89	12.5
90	7.0
91	5.5
92	4.0
93	3.0
94	2.0
95	1.0
96	0.0
97	0.5
98	1.0
99	0.5
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
50	733936.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	37.46219316068315
#Duplication Level	Percentage of deduplicated	Percentage of total
1	71.2511096870577	26.692228340095774
2	8.987124126623499	6.733547599812108
3	4.394519135601861	4.938849741187058
4	2.8271749425732335	4.23648695190887
5	2.034658213437725	3.8111379503887273
6	1.5529149966675717	3.4905360940369325
7	1.2268779222451403	3.2173076395407536
8	1.0532490463719797	3.1565615377193943
9	0.8704842364968037	2.9349223749875755
>10	5.726320313182074	37.00343761030131
>50	0.06013739151864794	1.449845930515334
>100	0.013847425329038363	0.8464598177537306
>500	3.9564072368680997E-4	0.12316724015992815
>1k	7.912814473736199E-4	0.6238398481746542
>5k	3.9564072368680997E-4	0.7416713234179066
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CGTTTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	5004	0.6818033180004796	No Hit
CGTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	2145	0.29225981556974995	No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	2064	0.2812234309258573	No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATA	831	0.11322513134660243	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.00912886137210874	0.0
2	0.0	0.0	0.0	0.025206557520001744	0.0
3	0.0	0.0	0.0	0.03678794881297552	0.0
4	0.0	0.0	0.0	0.05354690327221992	0.0
5	0.0	0.0	0.0	0.08597479889254649	0.0
6	0.0	0.0	0.0	0.14905931852368598	0.0
7	0.0	0.0	0.0	0.19388611541060802	0.0
8	0.0	0.0	0.0	0.39962612543873033	0.0
9	0.0	0.0	0.0	0.498817335571494	0.0
10	0.0	0.0	0.0	0.6593217937258835	0.0
11	0.0	0.0	0.0	0.8121961587931372	0.0
12	0.0	0.0	0.0	0.9457227878180113	0.0
13	0.0	0.0	0.0	1.0115323406945564	0.0
14	0.0	0.0	0.0	1.0397364347845044	0.0
15	0.0	0.0	0.0	1.0735268470275336	0.0
16	0.0	0.0	0.0	1.1536428244424581	0.0
17	0.0	0.0	0.0	1.2385276100368425	0.0
18	0.0	0.0	0.0	1.3724629940485273	0.0
19	0.0	0.0	0.0	1.4288711822284232	0.0
20	0.0	0.0	0.0	1.4895031719386977	0.0
21	0.0	0.0	0.0	1.5679841294063788	0.0
22	0.0	0.0	0.0	1.646056331887249	0.0
23	0.0	0.0	0.0	1.744975038695472	0.0
24	0.0	0.0	0.0	1.8205947112554772	0.0
25	0.0	0.0	0.0	1.8791829260316977	0.0
26	0.0	0.0	0.0	1.944174968934621	0.0
27	0.0	0.0	0.0	2.0188408798587343	0.0
28	0.0	0.0	0.0	2.08492293605982	0.0
29	0.0	0.0	0.0	2.1604063569575547	0.0
30	0.0	0.0	0.0	2.258780057116697	0.0
31	0.0	0.0	0.0	2.343664842711081	0.0
32	0.0	0.0	0.0	2.4286858799677358	0.0
33	0.0	0.0	0.0	2.5157506921584445	0.0
34	0.0	0.0	0.0	2.611535610734451	0.0
35	0.0	0.0	0.0	2.7276220269887292	0.0
36	0.0	0.0	0.0	2.8198644023457087	0.0
37	0.0	0.0	0.0	2.9187831091539316	0.0
38	0.0	0.0	0.0	3.033098253798696	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TCGTTAG	25	4.4412634E-5	44.0	2
GACGTAA	20	7.854619E-4	44.0	1
ATTAACG	25	4.4412634E-5	44.0	1
GTTCGAT	35	1.4451325E-7	44.0	39
CGTAAAT	20	7.854619E-4	44.0	16
CGACTTA	30	2.5264617E-6	44.0	17
GATTACG	20	7.854619E-4	44.0	1
AGTACGG	55	1.8189894E-12	44.0	2
TAGTCCA	25	4.4412634E-5	44.0	29
CGTTTTA	2865	0.0	43.078533	1
CGTTATT	1685	0.0	42.824924	1
AGGCGAT	470	0.0	42.59574	7
TAACGGG	185	0.0	41.621624	3
GTTTTAT	3175	0.0	41.36693	2
GTTATTT	1920	0.0	40.677082	2
ACGGGAT	130	0.0	40.615387	5
ACTAACC	185	0.0	40.432434	14
GTTGATC	525	0.0	40.22857	16
ACGGTCT	175	0.0	40.22857	30
AGGTACC	315	0.0	39.11111	7
>>END_MODULE