FastQCFastQC Report
Thu 26 May 2016
SRR1545703_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1545703_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences279274
Sequences flagged as poor quality0
Sequence length51
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT13280.4755186662560783No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT12480.44687296346956756No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATAC3390.12138616555783925No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGTTTA253.8831968E-545.00000438
AGGTACC1000.045.0000047
CTCGTGC253.8831968E-545.00000444
CGTATGG253.8831968E-545.0000042
ATAGGCC302.1595806E-645.0000045
TAGGTAC253.8831968E-545.0000046
AACTTCG302.1595806E-645.00000411
CACGACG600.045.00000426
GTTCGAT253.8831968E-545.00000439
AATTGGT302.1595806E-645.00000413
ATGGTCG253.8831968E-545.00000444
ATACGAA502.1827873E-1145.00000419
GATTAGT253.8831968E-545.00000443
TCACGAC551.8189894E-1245.025
CGACGCT351.2075179E-745.012
CGTGCGA207.0241076E-445.045
CGGTTTA207.0241076E-445.01
CAACCCG207.0241076E-445.023
TGCGCAC207.0241076E-445.043
TCGCTAT207.0241076E-445.023