##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1545703_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	279274
Sequences flagged as poor quality	0
Sequence length	51
%GC	45
>>END_MODULE
>>Per base sequence quality	fail
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	30.97215995760436	31.0	31.0	33.0	30.0	33.0
2	31.14773305069573	31.0	31.0	33.0	30.0	34.0
3	31.05873801356374	31.0	31.0	34.0	28.0	34.0
4	35.02051748462084	35.0	35.0	37.0	32.0	37.0
5	32.75978071714517	35.0	35.0	37.0	28.0	37.0
6	33.963806154529244	35.0	35.0	37.0	28.0	37.0
7	15.759730587165294	0.0	0.0	35.0	0.0	37.0
8	25.129127666735894	17.0	17.0	35.0	17.0	37.0
9	32.7505854465507	32.0	32.0	37.0	27.0	39.0
10	35.51338828533984	37.0	34.0	37.0	32.0	39.0
11	36.43827925263361	37.0	35.0	39.0	32.0	39.0
12	36.33773283585296	37.0	35.0	39.0	32.0	39.0
13	36.06815886906765	37.0	35.0	39.0	32.0	39.0
14	37.10262681094552	39.0	36.0	40.0	31.0	41.0
15	37.4286435543588	39.0	36.0	41.0	32.0	41.0
16	37.44580949175362	39.0	36.0	41.0	32.0	41.0
17	37.39929245114117	39.0	35.0	41.0	32.0	41.0
18	37.29299182881328	39.0	36.0	40.0	32.0	41.0
19	37.204924196309	39.0	36.0	40.0	32.0	41.0
20	37.2775553757242	39.0	35.0	40.0	32.0	41.0
21	37.14231543215623	39.0	35.0	40.0	32.0	41.0
22	37.18524817920752	39.0	35.0	40.0	32.0	41.0
23	37.149426727872985	39.0	35.0	40.0	32.0	41.0
24	36.93502796536735	39.0	35.0	40.0	32.0	41.0
25	36.69512736595601	38.0	35.0	40.0	31.0	41.0
26	36.57671319206228	38.0	35.0	40.0	31.0	41.0
27	36.47247864104786	38.0	35.0	40.0	31.0	41.0
28	36.360749658041925	38.0	35.0	40.0	31.0	41.0
29	36.311518437090456	38.0	35.0	40.0	30.0	41.0
30	36.02954446171144	38.0	35.0	40.0	30.0	41.0
31	35.84364459276553	38.0	34.0	40.0	30.0	41.0
32	35.676525562709024	38.0	34.0	40.0	29.0	41.0
33	35.408183361143536	38.0	34.0	40.0	27.0	41.0
34	35.159796472281705	38.0	34.0	40.0	25.0	41.0
35	34.9122868580677	38.0	34.0	40.0	24.0	41.0
36	34.696788816717635	38.0	34.0	40.0	23.0	41.0
37	34.54463000494138	38.0	33.0	40.0	23.0	41.0
38	34.41021362532853	38.0	33.0	40.0	23.0	41.0
39	34.319507007455044	37.0	33.0	40.0	23.0	41.0
40	34.12201637101914	37.0	33.0	40.0	21.0	41.0
41	33.942339781003604	37.0	33.0	40.0	21.0	41.0
42	33.88702492892285	37.0	33.0	40.0	20.0	41.0
43	33.80553864663377	37.0	33.0	40.0	20.0	41.0
44	33.57661651281537	37.0	33.0	40.0	19.0	41.0
45	33.505220679332844	36.0	33.0	40.0	20.0	41.0
46	33.47880217993798	36.0	32.0	40.0	20.0	41.0
47	33.406389424006534	36.0	32.0	40.0	20.0	41.0
48	33.34977119244899	36.0	32.0	40.0	20.0	41.0
49	33.22487234758696	36.0	32.0	40.0	18.0	41.0
50	33.10930484040763	36.0	32.0	40.0	18.0	40.0
51	32.909042732227135	35.0	32.0	39.0	18.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
13	5.0
14	4.0
15	15.0
16	36.0
17	110.0
18	218.0
19	435.0
20	721.0
21	1156.0
22	1780.0
23	2643.0
24	3833.0
25	5044.0
26	6060.0
27	6247.0
28	6412.0
29	6926.0
30	8400.0
31	10578.0
32	13656.0
33	20037.0
34	25488.0
35	27893.0
36	32829.0
37	48512.0
38	41548.0
39	8688.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	33.32211376640862	23.124601645695623	26.839591225821234	16.713693362074522
2	36.072817376483314	22.573888009624955	28.99374807536684	12.359546538524889
3	30.764052507573208	22.542735807844625	33.92868652291298	12.764525161669185
4	26.18682727357362	26.782657891533045	31.787420239621305	15.243094595272028
5	21.085027607296063	35.33160981688234	30.14423111353008	13.439131462291511
6	19.91341836332777	40.22429585281838	30.02212880540258	9.84015697845127
7	37.786188474401484	55.53685627734769	5.857688148556615	0.8192670996942072
8	84.85000393878414	3.38878663964422	10.084003523421444	1.6772058981502038
9	80.53810952684461	4.730479743907416	11.644120111431784	3.087290617816195
10	48.61426412770255	21.947621332454865	17.606365075159164	11.83174946468343
11	40.83659774988004	21.884600786324544	23.169002485014715	14.109798978780697
12	36.11650207323274	21.406933692359477	27.715791659803635	14.760772574604154
13	22.468973123169363	33.68842069079112	27.835029397652484	16.00757678838703
14	17.739567593116437	35.89807858948559	31.33231163660061	15.030042180797354
15	16.178734862536434	22.883619671004105	44.74530389509944	16.19234157136003
16	18.861762999777994	18.83884643754879	43.09101455917844	19.208376003494777
17	19.51452695202561	18.328952927948897	31.15399213675458	31.002527983270912
18	22.5846301481699	22.511941677349125	35.1565129585998	19.746915215881177
19	28.640331717238265	23.36629976295681	27.754105287280588	20.239263232524333
20	29.79403739696499	23.29110479314222	27.087018483639724	19.82783932625307
21	23.59474924267923	25.70199875391193	31.004318339695065	19.69893366371377
22	23.789181950342673	21.637173528506054	29.062855833339302	25.51078868781197
23	20.810028860545557	27.02113336723075	28.163380765842867	24.00545700638083
24	20.498506842742252	23.745497253593246	37.48218595357964	18.273809950084864
25	21.112599096228077	23.01467376125239	34.82279052113695	21.049936621382585
26	18.49044307740785	29.65510573845041	29.739252490385788	22.115198693755953
27	18.704211634452186	29.72242313999871	30.793414352929382	20.77995087261972
28	17.715576817032737	26.591089754148257	37.23619098090048	18.45714244791853
29	18.988878305893138	24.321992022171774	35.944985927798506	20.744143744136583
30	20.889878757062956	27.070189133252647	32.96010369744409	19.07982841224031
31	28.37321053875406	24.24643898107235	28.01012625593503	19.370224224238562
32	29.001983714917966	24.55903521273015	28.510709912129307	17.92827116022258
33	27.618396270329498	24.921761424264343	26.97709059919649	20.482751706209672
34	21.222885051956144	26.306065011422476	29.394071771808335	23.07697816481305
35	21.38723977169375	25.540866675737806	31.06232588783775	22.009567664730696
36	29.21718455710163	25.19568595716035	26.526636922878605	19.060492562859412
37	21.39762383895386	30.88615481570071	28.088185796028277	19.628035549317158
38	22.378739159391852	31.204838259200642	24.873063729527274	21.54335885188023
39	21.807257388800963	28.851951846573616	26.318955577676405	23.02183518694902
40	24.28725910754313	24.634946325114406	27.7279660834879	23.349828483854566
41	19.905182723776647	24.10607503741845	28.642122073662424	27.346620165142475
42	23.193709403668084	25.453855353523778	25.191389101742374	26.161046141065764
43	22.06005571589192	25.882466681466948	26.695288498034188	25.362189104606948
44	21.293067023783095	27.9059275120491	28.9099593947163	21.891046069451505
45	19.25635755566218	33.00056575263003	25.742818880382707	22.000257811325078
46	22.27454041550592	29.051039480939867	27.734053295330035	20.94036680822418
47	21.82444481047287	27.985061265996837	27.43291534478684	22.757578578743455
48	22.714968095848523	25.95300672457873	29.894655428002608	21.43736975157014
49	22.237659073168288	25.320294764281677	30.926616870886658	21.515429291663384
50	20.57191145613269	30.402400509893507	27.591182852682312	21.43450518129149
51	20.230669521688377	31.31763071392253	26.47006165987525	21.981638104513845
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	248.0
1	355.5
2	463.0
3	2595.0
4	4727.0
5	3440.5
6	2154.0
7	2122.5
8	2091.0
9	2114.5
10	2138.0
11	2114.0
12	2090.0
13	2004.0
14	1918.0
15	1829.5
16	1741.0
17	1628.0
18	1515.0
19	1458.0
20	1401.0
21	1440.0
22	1479.0
23	1570.0
24	1661.0
25	1910.5
26	2356.0
27	2552.0
28	2919.5
29	3287.0
30	3844.0
31	4401.0
32	5092.5
33	5784.0
34	6500.5
35	7217.0
36	7936.0
37	8655.0
38	10930.0
39	13205.0
40	16391.0
41	19577.0
42	20645.0
43	21713.0
44	21698.0
45	21683.0
46	21375.0
47	21067.0
48	20216.5
49	19366.0
50	18592.5
51	17819.0
52	16578.5
53	15338.0
54	14354.0
55	13370.0
56	12937.5
57	12505.0
58	11770.0
59	11035.0
60	10055.5
61	9076.0
62	8133.5
63	7191.0
64	6433.5
65	5676.0
66	4892.0
67	4108.0
68	3629.5
69	3151.0
70	2689.5
71	2228.0
72	1870.0
73	1512.0
74	1210.5
75	712.0
76	515.0
77	390.5
78	266.0
79	209.5
80	153.0
81	113.0
82	73.0
83	51.5
84	30.0
85	22.5
86	15.0
87	11.0
88	7.0
89	5.0
90	3.0
91	2.0
92	1.0
93	0.5
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	279274.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	50.18286722107852
#Duplication Level	Percentage of deduplicated	Percentage of total
1	71.98558373337993	36.12442990324035
2	10.144937770927754	10.182041302491438
3	5.396327044230736	8.124094906264384
4	3.3754224285061967	6.775535021791075
5	2.353192263695512	5.904496745735054
6	1.6699305174906758	5.028114085659691
7	1.3018400572278113	4.573104671446116
8	0.902922048325047	3.6248973809664067
9	0.6555924191017728	2.9609556587036936
>10	2.193015732013855	14.899550557155194
>50	0.017696654250607787	0.6195168301196724
>100	0.0021235985100729344	0.2681967928557068
>500	0.0	0.0
>1k	0.001415732340048623	0.9150661435712591
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CGTTTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1328	0.4755186662560783	No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1248	0.44687296346956756	No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATAC	339	0.12138616555783925	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.007877568266290453	0.0
2	0.0	0.0	0.0	0.024706918653365512	0.0
3	0.0	0.0	0.0	0.03115220178033043	0.0
4	0.0	0.0	0.0	0.03974591261628365	0.0
5	0.0	0.0	0.0	0.07018197182695131	0.0
6	0.0	0.0	0.0	0.11708931013986264	0.0
7	0.0	0.0	0.0	0.14215430007805954	0.0
8	0.0	0.0	0.0	0.295408809985892	0.0
9	0.0	0.0	0.0	0.3695295659459885	0.0
10	0.0	0.0	0.0	0.49413837306731023	0.0
11	0.0	0.0	0.0	0.626266677170091	0.0
12	0.0	0.0	0.0	0.7383429893223142	0.0
13	0.0	0.0	0.0	0.785608398920057	0.0
14	0.0	0.0	0.0	0.8121056739975795	0.0
15	0.0	0.0	0.0	0.8418255906385843	0.0
16	0.0	0.0	0.0	0.8933878556543037	0.0
17	0.0	0.0	0.0	0.961779471057098	0.0
18	0.0	0.0	0.0	1.0774364960576353	0.0
19	0.0	0.0	0.0	1.1318633313520055	0.0
20	0.0	0.0	0.0	1.1737576716772775	0.0
21	0.0	0.0	0.0	1.23391364752895	0.0
22	0.0	0.0	0.0	1.3055279044952268	0.0
23	0.0	0.0	0.0	1.3928972979940846	0.0
24	0.0	0.0	0.0	1.4526952025609259	0.0
25	0.0	0.0	0.0	1.5067639665704649	0.0
26	0.0	0.0	0.0	1.562981158288992	0.0
27	0.0	0.0	0.0	1.633521201400775	0.0
28	0.0	0.0	0.0	1.6915287495434592	0.0
29	0.0	0.0	0.0	1.7717367173456893	0.0
30	0.0	0.0	0.0	1.8655513939715118	0.0
31	0.0	0.0	0.0	1.9396721499316083	0.0
32	0.0	0.0	0.0	2.012002549467548	0.0
33	0.0	0.0	0.0	2.0936428024091036	0.0
34	0.0	0.0	0.0	2.1831606236169496	0.0
35	0.0	0.0	0.0	2.285210939793894	0.0
36	0.0	0.0	0.0	2.3607639808933163	0.0
37	0.0	0.0	0.0	2.4517140872404877	0.0
38	0.0	0.0	0.0	2.55054176185395	0.0
39	0.0	0.0	0.0	2.715970695446049	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TCGTTTA	25	3.8831968E-5	45.000004	38
AGGTACC	100	0.0	45.000004	7
CTCGTGC	25	3.8831968E-5	45.000004	44
CGTATGG	25	3.8831968E-5	45.000004	2
ATAGGCC	30	2.1595806E-6	45.000004	5
TAGGTAC	25	3.8831968E-5	45.000004	6
AACTTCG	30	2.1595806E-6	45.000004	11
CACGACG	60	0.0	45.000004	26
GTTCGAT	25	3.8831968E-5	45.000004	39
AATTGGT	30	2.1595806E-6	45.000004	13
ATGGTCG	25	3.8831968E-5	45.000004	44
ATACGAA	50	2.1827873E-11	45.000004	19
GATTAGT	25	3.8831968E-5	45.000004	43
TCACGAC	55	1.8189894E-12	45.0	25
CGACGCT	35	1.2075179E-7	45.0	12
CGTGCGA	20	7.0241076E-4	45.0	45
CGGTTTA	20	7.0241076E-4	45.0	1
CAACCCG	20	7.0241076E-4	45.0	23
TGCGCAC	20	7.0241076E-4	45.0	43
TCGCTAT	20	7.0241076E-4	45.0	23
>>END_MODULE