FastQCFastQC Report
Thu 26 May 2016
SRR1545702_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1545702_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences919324
Sequences flagged as poor quality0
Sequence length50
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT57390.6242630454551388No Hit
CGTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT25580.27824793000073966No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT23740.25823322354251604No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTCTA207.855657E-444.0000041
ATTACGG351.44566E-744.02
CGAATAT551.8189894E-1244.014
CATACCG254.4421417E-544.012
CGTTTTA33700.043.869441
CGTTATT16950.042.961651
TACGGGA1650.042.6666684
ACGGGTA1000.041.8000035
CATAGGT850.041.4117624
GTTTTAT38000.041.2789462
AGGCGAT7100.041.2112667
TACGGGT650.040.6153834
CGGGTAT557.8216544E-1140.06
CTCACGA1650.040.024
TTACGGG2100.039.809523
CGGTCTA1550.039.74193231
CGTTTTT14650.039.344711
GTTATTT19600.039.0612262
GGCGATT3900.037.7948728
TCACGAC1750.037.71428725
Produced by FastQC (version 0.11.5)