##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1545702_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	919324
Sequences flagged as poor quality	0
Sequence length	50
%GC	45
>>END_MODULE
>>Per base sequence quality	fail
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.446686913427694	31.0	31.0	33.0	30.0	34.0
2	31.63734330877906	31.0	31.0	34.0	30.0	34.0
3	31.655108536272305	31.0	31.0	34.0	30.0	34.0
4	35.5917793944246	37.0	35.0	37.0	33.0	37.0
5	27.20341685847427	35.0	26.0	37.0	0.0	37.0
6	31.36675100399859	35.0	28.0	37.0	17.0	37.0
7	14.904851825906862	0.0	0.0	32.0	0.0	37.0
8	25.030886825537024	17.0	17.0	35.0	17.0	37.0
9	33.08099647132023	32.0	32.0	37.0	28.0	39.0
10	35.87033951033585	37.0	35.0	39.0	32.0	39.0
11	36.864711461900264	38.0	37.0	39.0	33.0	39.0
12	37.04841927329211	39.0	37.0	39.0	34.0	39.0
13	36.72455195339184	39.0	35.0	39.0	33.0	39.0
14	38.06203471246263	40.0	37.0	41.0	33.0	41.0
15	38.28619181050424	40.0	37.0	41.0	33.0	41.0
16	38.307394346280525	40.0	37.0	41.0	34.0	41.0
17	38.305514704282714	40.0	37.0	41.0	34.0	41.0
18	38.260510984157925	40.0	37.0	41.0	34.0	41.0
19	38.21178278822265	40.0	37.0	41.0	34.0	41.0
20	38.24025262040369	40.0	37.0	41.0	34.0	41.0
21	38.11190940299611	40.0	37.0	41.0	34.0	41.0
22	37.992613050458814	40.0	36.0	41.0	34.0	41.0
23	37.86279266069416	40.0	36.0	41.0	33.0	41.0
24	37.70093786303849	40.0	35.0	41.0	33.0	41.0
25	37.50660267761964	39.0	35.0	41.0	33.0	41.0
26	37.25100617410184	39.0	35.0	40.0	33.0	41.0
27	37.06065978914942	39.0	35.0	40.0	32.0	41.0
28	37.05157485282664	39.0	35.0	40.0	32.0	41.0
29	36.93059356657718	39.0	35.0	40.0	32.0	41.0
30	36.69597225787644	38.0	35.0	40.0	31.0	41.0
31	36.50389851673621	38.0	35.0	40.0	31.0	41.0
32	36.275411062911445	38.0	35.0	40.0	30.0	41.0
33	36.027747562339286	38.0	35.0	40.0	30.0	41.0
34	35.71320557279044	38.0	35.0	40.0	29.0	41.0
35	35.456551770648865	38.0	35.0	40.0	27.0	41.0
36	35.27532186693701	38.0	35.0	40.0	26.0	41.0
37	35.16392697242757	38.0	34.0	40.0	25.0	41.0
38	34.961357475710415	38.0	34.0	40.0	25.0	41.0
39	34.849628640174735	38.0	34.0	40.0	24.0	41.0
40	34.61286771584338	38.0	34.0	40.0	24.0	41.0
41	34.38092119861985	37.0	34.0	40.0	23.0	40.0
42	34.370483094099576	37.0	34.0	40.0	23.0	40.0
43	34.22898129495151	37.0	33.0	40.0	23.0	40.0
44	33.99549234002376	37.0	33.0	40.0	23.0	40.0
45	33.80754119331161	37.0	33.0	39.0	23.0	40.0
46	33.71319469523258	36.0	33.0	39.0	22.0	40.0
47	33.53161888518085	36.0	33.0	39.0	22.0	40.0
48	33.42487414665558	36.0	33.0	39.0	21.0	40.0
49	33.27780195012857	36.0	33.0	39.0	20.0	40.0
50	33.358477533492	36.0	33.0	39.0	20.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
9	2.0
10	1.0
11	0.0
12	1.0
13	2.0
14	5.0
15	24.0
16	71.0
17	152.0
18	421.0
19	801.0
20	1523.0
21	2543.0
22	4189.0
23	6244.0
24	9351.0
25	13719.0
26	16944.0
27	17877.0
28	18124.0
29	19631.0
30	23099.0
31	29870.0
32	39861.0
33	65336.0
34	73391.0
35	95092.0
36	132812.0
37	228439.0
38	94209.0
39	25590.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	34.81895392701594	21.127589402648034	25.94645630920111	18.10700036113492
2	36.69228694127424	22.64881586905161	28.58752735705801	12.07136983261614
3	30.565176151171947	23.34878671719655	33.923948466481896	12.16208866514961
4	26.413538643612043	26.291818771184044	32.74808446206126	14.546558123142658
5	17.04535071422045	46.892499271203626	24.6563779472743	11.405772067301626
6	19.72895301330108	39.87582179949615	30.355783162410642	10.039442024792129
7	37.73718514908781	55.912931675883584	5.198058573473553	1.1518246015550557
8	84.14291370615801	2.9905669818257765	10.553297857991307	2.313221454024914
9	79.3735396878576	4.776879533222237	12.389864726690481	3.4597160522296817
10	41.96159351871593	25.502543173027135	19.785625089739852	12.750238218517085
11	34.32380749333206	23.88113439875387	26.566150780356	15.228907327558074
12	31.089909542228856	22.200769260891697	30.168797942836257	16.540523254043187
13	23.16158394646501	28.23183121511023	31.193463893034444	17.413120945390308
14	18.687753175159138	31.29897620425443	32.68782279152943	17.325447829057
15	17.839086111099025	23.25752400677019	40.78801380144541	18.115376080685373
16	20.5838202853401	20.19375106056189	39.26765753967045	19.954771114427558
17	20.445022647075458	20.41880773263833	32.3343021611532	26.801867459133017
18	22.45225839856242	22.87343743881374	34.1994770070182	20.47482715560564
19	26.351101461508676	24.676827756046833	28.819763217320553	20.15230756512394
20	27.432657039302793	23.98621160765954	28.50300873250345	20.07812262053422
21	23.78182229551279	25.496669291783963	30.949153943549824	19.772354469153424
22	23.284391574678786	22.639787496029694	30.494363249518123	23.5814576797734
23	21.394198345741	26.57626690916369	29.300333723475074	22.729201021620234
24	21.63578890576119	23.713837558901975	34.79524085088608	19.855132684450748
25	21.752505101574634	24.41206799778968	32.49441981281899	21.3410070878167
26	20.0087238014019	27.271560407429806	30.242656560690246	22.477059230478048
27	20.23932802798578	26.49000787535189	31.28505293019653	21.985611166465794
28	19.92333497221872	25.15685438430847	34.70299916025253	20.21681148322028
29	20.096832020049515	24.068772271799713	34.623701763469676	21.21069394468109
30	21.754245510831872	25.163924796915992	32.322554398666846	20.759275293585286
31	25.802872545479072	24.579908715534458	28.383464371646994	21.23375436733948
32	25.782857839020846	24.74415983918618	28.26979389203371	21.20318842975926
33	25.0095722509148	24.73056289186402	28.57621469688597	21.6836501603352
34	21.012613616091823	25.908167305541895	30.28355617823531	22.795662900130967
35	21.644164625311642	24.79876517963199	31.356409709743243	22.20066048531312
36	25.75359720838355	25.227232183647985	28.044302117642967	20.9748684903255
37	22.248630515465713	28.430455421592388	28.49811383146747	20.82280023147443
38	22.910421135530022	28.17374505614995	27.324099011882648	21.591734796437382
39	22.39460734191645	27.644551866371376	27.17268340650304	22.788157385209132
40	23.91202666306982	24.885241764600945	28.78930605531891	22.413425517010324
41	20.976391348425583	24.760258624815627	29.05906949019062	25.204280536568174
42	22.7605283882505	26.554403017869653	26.349904930144323	24.335163663735525
43	22.325969951834175	26.03793657078462	27.83273361731011	23.803359860071097
44	22.053704678655187	27.146794818801638	28.678681291905793	22.120819210637382
45	21.09191101287468	28.86718936958026	27.129499501807853	22.911400115737216
46	22.74867185018557	27.634544513142266	28.52411119474744	21.092672441924716
47	22.096127154300333	27.026924131209455	28.477772798273516	22.3991759162167
48	22.007801384495565	25.883801575940584	29.580648389468784	22.52774865009507
49	22.053704678655187	25.155331526208386	30.18565815751574	22.60530563762069
50	21.24582845656156	27.242626103528245	29.24975307943663	22.261792360473564
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	658.0
1	1039.5
2	1421.0
3	9531.0
4	17641.0
5	12431.0
6	7221.0
7	7093.5
8	6966.0
9	7046.5
10	7127.0
11	7218.5
12	7310.0
13	6818.0
14	6326.0
15	6078.0
16	5830.0
17	5553.0
18	5276.0
19	5216.0
20	5156.0
21	5230.0
22	5304.0
23	5642.0
24	5980.0
25	6852.5
26	7725.0
27	9321.5
28	10918.0
29	12181.0
30	13444.0
31	15655.0
32	17866.0
33	20119.5
34	22373.0
35	24454.0
36	26535.0
37	30180.0
38	33825.0
39	40193.0
40	46561.0
41	53089.5
42	59618.0
43	63390.5
44	67163.0
45	68721.0
46	70279.0
47	70653.0
48	71027.0
49	68763.0
50	66499.0
51	60873.5
52	55248.0
53	51498.5
54	47749.0
55	45662.0
56	43575.0
57	41604.5
58	39634.0
59	38014.0
60	36394.0
61	33056.0
62	29718.0
63	25923.5
64	22129.0
65	18765.5
66	15402.0
67	13473.0
68	11544.0
69	10153.5
70	8763.0
71	7335.0
72	5907.0
73	4595.0
74	3283.0
75	2593.0
76	1903.0
77	1433.0
78	963.0
79	771.0
80	579.0
81	426.0
82	273.0
83	187.0
84	101.0
85	77.5
86	54.0
87	42.0
88	30.0
89	21.5
90	13.0
91	8.5
92	4.0
93	4.0
94	4.0
95	4.5
96	5.0
97	2.5
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
50	919324.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	34.68702913315629
#Duplication Level	Percentage of deduplicated	Percentage of total
1	67.61727890897387	23.454425234203306
2	8.89567444301764	6.171290371280534
3	4.948019390469875	5.148962782459522
4	3.396669660072501	4.712815178185716
5	2.5283923913030173	4.385121026858923
6	1.999170598706403	4.16071732796871
7	1.5484883682206003	3.7598722798575124
8	1.3041259839103172	3.6189004797762623
9	1.1025887641748164	3.4420975726340384
>10	6.591558263108056	37.72569950104798
>50	0.051113064633052915	1.146432581115679
>100	0.01551014979242542	0.884400309338579
>500	3.5250340437256717E-4	0.08449061437192534
>1k	7.050068087451343E-4	0.603050231667635
>5k	3.5250340437256717E-4	0.7017245092336897
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CGTTTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	5739	0.6242630454551388	No Hit
CGTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	2558	0.27824793000073966	No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	2374	0.25823322354251604	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.007614290500411172	0.0
2	0.0	0.0	0.0	0.02926063063729436	0.0
3	0.0	0.0	0.0	0.04383655816665289	0.0
4	0.0	0.0	0.0	0.07037779933951469	0.0
5	0.0	0.0	0.0	0.12302517937092908	0.0
6	0.0	0.0	0.0	0.2708511906574831	0.0
7	0.0	0.0	0.0	0.3850655481636507	0.0
8	0.0	0.0	0.0	0.9820259233958866	0.0
9	0.0	0.0	0.0	1.2772428436546854	0.0
10	0.0	0.0	0.0	1.6685085997972424	0.0
11	0.0	0.0	0.0	1.883775469801724	0.0
12	0.0	0.0	0.0	2.0954527457131547	0.0
13	0.0	0.0	0.0	2.19563505358285	0.0
14	0.0	0.0	0.0	2.2405593675352757	0.0
15	0.0	0.0	0.0	2.28918205115933	0.0
16	0.0	0.0	0.0	2.3763112895997494	0.0
17	0.0	0.0	0.0	2.463658079197323	0.0
18	0.0	0.0	0.0	2.6162702159412787	0.0
19	0.0	0.0	0.0	2.68033903172331	0.0
20	0.0	0.0	0.0	2.7553941809416482	0.0
21	0.0	0.0	0.0	2.842305868224913	0.0
22	0.0	0.0	0.0	2.9342212321227334	0.0
23	0.0	0.0	0.0	3.0377755829283255	0.0
24	0.0	0.0	0.0	3.1258838015759407	0.0
25	0.0	0.0	0.0	3.193977313765332	0.0
26	0.0	0.0	0.0	3.2682710339336296	0.0
27	0.0	0.0	0.0	3.3452841435663596	0.0
28	0.0	0.0	0.0	3.4217533753062033	0.0
29	0.0	0.0	0.0	3.511928329946787	0.0
30	0.0	0.0	0.0	3.6350622848962932	0.0
31	0.0	0.0	0.0	3.733830510244484	0.0
32	0.0	0.0	0.0	3.8212860754206353	0.0
33	0.0	0.0	0.0	3.9102644986968684	0.0
34	0.0	0.0	0.0	4.008380070573596	0.0
35	0.0	0.0	0.0	4.125640144279927	0.0
36	0.0	0.0	0.0	4.221253877849376	0.0
37	0.0	0.0	0.0	4.319043122990371	0.0
38	0.0	0.0	0.0	4.439784015211177	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGTTCTA	20	7.855657E-4	44.000004	1
ATTACGG	35	1.44566E-7	44.0	2
CGAATAT	55	1.8189894E-12	44.0	14
CATACCG	25	4.4421417E-5	44.0	12
CGTTTTA	3370	0.0	43.86944	1
CGTTATT	1695	0.0	42.96165	1
TACGGGA	165	0.0	42.666668	4
ACGGGTA	100	0.0	41.800003	5
CATAGGT	85	0.0	41.411762	4
GTTTTAT	3800	0.0	41.278946	2
AGGCGAT	710	0.0	41.211266	7
TACGGGT	65	0.0	40.615383	4
CGGGTAT	55	7.8216544E-11	40.0	6
CTCACGA	165	0.0	40.0	24
TTACGGG	210	0.0	39.80952	3
CGGTCTA	155	0.0	39.741932	31
CGTTTTT	1465	0.0	39.34471	1
GTTATTT	1960	0.0	39.061226	2
GGCGATT	390	0.0	37.794872	8
TCACGAC	175	0.0	37.714287	25
>>END_MODULE