FastQCFastQC Report
Thu 26 May 2016
SRR1545701_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1545701_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences345757
Sequences flagged as poor quality0
Sequence length51
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT14630.42312953895365824No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT13900.40201644507558776No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCGATA253.8851285E-545.0000047
AGGCCGA207.026438E-445.07
AGTACGG207.026438E-445.02
CGTTTTA8400.043.6607131
AGGCGAT2500.043.27
CGTTATT2200.042.9545441
CGTTTTT12200.042.786881
TAGGTAC750.042.06
ACGGGCT603.6379788E-1241.2499965
CAACCCG556.002665E-1140.90909223
GTTAGGT556.002665E-1140.9090924
GACCCGA451.9215804E-840.040
GTTTTAT10000.039.8252
GTAAGGT850.039.7058834
GTAAGGC850.039.7058834
GACGTCG403.4488767E-739.37519
TCGCTAT403.4488767E-739.37523
GGCGATA800.039.3758
GTACGGG750.039.03
GCGACGT356.235623E-638.5714317