##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1545701_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	345757
Sequences flagged as poor quality	0
Sequence length	51
%GC	45
>>END_MODULE
>>Per base sequence quality	fail
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	30.92616779992885	31.0	31.0	33.0	30.0	33.0
2	31.106262490708794	31.0	31.0	33.0	30.0	34.0
3	31.00299343180326	31.0	31.0	34.0	28.0	34.0
4	34.98933933369389	35.0	35.0	37.0	32.0	37.0
5	32.74971728699636	35.0	35.0	37.0	28.0	37.0
6	33.94159481948305	35.0	35.0	37.0	28.0	37.0
7	15.162298955624903	0.0	0.0	35.0	0.0	37.0
8	24.818942205074663	17.0	17.0	35.0	17.0	37.0
9	32.5759044646963	32.0	32.0	37.0	27.0	39.0
10	35.30341540446036	37.0	34.0	37.0	32.0	39.0
11	36.287473572480096	37.0	35.0	39.0	32.0	39.0
12	36.337173217028145	37.0	35.0	39.0	32.0	39.0
13	36.2144598663223	38.0	35.0	39.0	32.0	39.0
14	37.28759793727965	39.0	36.0	40.0	32.0	41.0
15	37.537371622266505	39.0	36.0	41.0	32.0	41.0
16	37.51067657343163	39.0	36.0	41.0	32.0	41.0
17	37.42531893786677	39.0	36.0	41.0	32.0	41.0
18	37.344421081858066	39.0	36.0	40.0	32.0	41.0
19	37.29576552318536	39.0	36.0	40.0	32.0	41.0
20	37.38662991638636	39.0	35.0	40.0	32.0	41.0
21	37.2740016832631	39.0	35.0	40.0	32.0	41.0
22	37.28572089646775	39.0	35.0	40.0	32.0	41.0
23	37.24349181650697	39.0	35.0	40.0	32.0	41.0
24	37.06152586932441	39.0	35.0	40.0	32.0	41.0
25	36.79761798025781	38.0	35.0	40.0	31.0	41.0
26	36.661059645936305	38.0	35.0	40.0	31.0	41.0
27	36.59668784724532	38.0	35.0	40.0	31.0	41.0
28	36.47012497216253	38.0	35.0	40.0	30.0	41.0
29	36.401935463345644	38.0	35.0	40.0	30.0	41.0
30	36.098783249507605	38.0	35.0	40.0	30.0	41.0
31	35.964642798265835	38.0	35.0	40.0	30.0	41.0
32	35.85757916687153	38.0	35.0	40.0	29.0	41.0
33	35.622726365626725	38.0	34.0	40.0	27.0	41.0
34	35.37233953325602	38.0	34.0	40.0	26.0	41.0
35	35.139164210702894	38.0	34.0	40.0	25.0	41.0
36	34.928469994822954	38.0	34.0	40.0	24.0	41.0
37	34.80573350647998	38.0	34.0	40.0	24.0	41.0
38	34.65190003383879	38.0	33.0	40.0	23.0	41.0
39	34.49522930844495	38.0	33.0	40.0	23.0	41.0
40	34.34923949478969	38.0	33.0	40.0	22.0	41.0
41	34.152558010394586	38.0	33.0	40.0	21.0	41.0
42	34.09262574582727	37.0	33.0	40.0	21.0	41.0
43	34.00573813400741	37.0	33.0	40.0	21.0	41.0
44	33.78166168725434	37.0	33.0	40.0	20.0	41.0
45	33.69035478674329	37.0	33.0	40.0	20.0	41.0
46	33.62897063544628	37.0	33.0	40.0	20.0	41.0
47	33.52815705828082	37.0	32.0	40.0	20.0	41.0
48	33.456815046405424	36.0	32.0	40.0	20.0	41.0
49	33.321289229140696	36.0	32.0	40.0	19.0	41.0
50	33.20956336386537	36.0	32.0	40.0	18.0	41.0
51	33.0348568503313	36.0	32.0	40.0	18.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
11	2.0
12	2.0
13	4.0
14	12.0
15	23.0
16	52.0
17	125.0
18	270.0
19	514.0
20	878.0
21	1534.0
22	2200.0
23	3361.0
24	4592.0
25	5979.0
26	6897.0
27	7238.0
28	7653.0
29	8669.0
30	10403.0
31	13029.0
32	16829.0
33	23571.0
34	29268.0
35	34678.0
36	42932.0
37	62847.0
38	50935.0
39	11260.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	34.613615921008105	21.819081030897422	26.191516006906586	17.375787041187888
2	36.213294307852046	22.528828049757486	29.099338552798642	12.158539089591823
3	31.167554091457294	23.26286958760054	33.20655836324355	12.363017957698615
4	27.02013263650483	26.847757239911267	31.289026686372225	14.843083437211682
5	20.697773291647025	35.5032580685279	29.712196716190853	14.086771923634227
6	19.81420477387298	40.71501083130638	29.679514803749452	9.791269591071186
7	36.36311050824712	57.10079622393762	5.661201363963709	0.8748919038515488
8	84.90327021578739	3.3697076270328585	9.544853755672337	2.1821684015074174
9	79.9954881607603	4.937282542363568	11.395575505340457	3.6716537915356744
10	41.891848899660744	26.32397897945667	18.985298923810653	12.798873197071932
11	34.71108321740413	24.37954979942561	25.79991149853799	15.109455484632269
12	31.51346176650075	22.65348206977733	29.312494034827928	16.520562128893992
13	23.417313315420945	29.156314984223027	30.036991297356234	17.389380402999794
14	18.65356305150727	32.36897589925873	31.79371639619733	17.18374465303667
15	17.789661525290885	23.627865813273484	40.314440488551206	18.268032172884425
16	20.57832523998068	20.646581269504306	38.86313219978193	19.91196129073309
17	20.408552827563867	20.984390771553432	31.626546967957264	26.980509432925437
18	22.57105423751363	23.28658566565536	33.69910081357717	20.443259283253845
19	26.761569541614488	25.153792981776217	27.957206940134256	20.12743053647504
20	27.831685258722167	24.459953088440724	27.718889277729737	19.989472375107372
21	23.672984205670456	26.328317286417917	30.347903296245633	19.650795211665997
22	23.265472571777288	23.218618856595818	29.766280942974397	23.74962762865249
23	21.5550227471895	27.108923318978356	28.641213337690925	22.694840596141223
24	21.308317691326568	24.415123916507834	34.466981145717945	19.80957724644765
25	21.779747047782113	24.965510459658084	32.14540848052245	21.109334012037355
26	19.83878851332005	28.0439730793592	29.66274001683263	22.454498390488116
27	20.177176456297342	27.306460895947154	30.547176195998926	21.96918645175658
28	19.864818355087532	26.03822916094251	34.250644238583746	19.84630824538621
29	20.006536382488278	24.649392492415192	34.256428647865405	21.08764247723112
30	21.5807633684929	26.248203217866884	31.79458405758958	20.376449356050635
31	26.159990976321524	25.134125990218564	27.861764186986814	20.8441188464731
32	26.318483790639092	25.045335307745038	27.69748696338758	20.93869393822829
33	25.5130047981675	25.17461685519021	27.87882819436772	21.433550152274574
34	21.05293602154114	26.285512657733612	29.711618275262687	22.949933045462565
35	21.71438322289932	24.86688628140573	31.237834664229503	22.180895831465453
36	26.10041156072039	25.692899926827224	27.343770335813883	20.862918176638505
37	22.059423236550526	29.155447322830774	28.095454321966006	20.689675118652694
38	22.830195773332136	28.871432827101113	26.85643385383376	21.441937545732987
39	22.371781337760336	28.292702678470715	26.660631599649463	22.67488438411948
40	23.981003999919018	25.23448549125542	28.39161607718716	22.392894431638403
41	20.829657823268942	25.0219084501543	28.70542028071738	25.443013445859375
42	22.612701984341605	26.928160528926387	25.823338356128726	24.635799130603285
43	22.054795709125194	26.18920224319392	27.572832943367743	24.183169104313144
44	21.933901555138434	27.36951095711728	28.600144031791114	22.096443455953168
45	20.847011051113935	29.528252501033965	26.81247234329312	22.81226410455898
46	22.50597963309492	28.378601156303418	28.124954809302487	20.990464401299178
47	22.031079631070376	27.61795133576471	27.84643550239041	22.504533530774502
48	21.871140714432393	26.395416434085213	29.308444948330763	22.424997903151635
49	21.979309167999492	25.647203093502085	30.164826742480994	22.208660996017436
50	21.152427861185746	28.19754914578736	28.68315030498298	21.966872688043914
51	20.746362329612992	29.04236212137426	27.585269423323318	22.62600612568943
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	265.0
1	360.0
2	455.0
3	2969.0
4	5483.0
5	3925.5
6	2368.0
7	2309.5
8	2251.0
9	2309.0
10	2367.0
11	2317.0
12	2267.0
13	2248.0
14	2229.0
15	2113.0
16	1997.0
17	1945.5
18	1894.0
19	1871.0
20	1848.0
21	1870.5
22	1893.0
23	2080.5
24	2268.0
25	2503.0
26	3282.5
27	3827.0
28	4378.0
29	4929.0
30	5651.0
31	6373.0
32	7339.0
33	8305.0
34	9062.5
35	9820.0
36	10971.5
37	12123.0
38	14136.0
39	16149.0
40	18823.5
41	21498.0
42	22779.5
43	24061.0
44	24551.5
45	25042.0
46	25693.0
47	26344.0
48	25578.0
49	24812.0
50	23635.5
51	22459.0
52	20916.5
53	19374.0
54	18308.0
55	17242.0
56	16387.0
57	15532.0
58	14736.0
59	13940.0
60	12846.0
61	11752.0
62	10519.0
63	9286.0
64	8128.0
65	6970.0
66	5931.0
67	4892.0
68	4294.0
69	3696.0
70	3292.0
71	2888.0
72	2363.5
73	1839.0
74	1447.0
75	798.5
76	542.0
77	430.0
78	318.0
79	245.5
80	173.0
81	133.0
82	93.0
83	74.0
84	55.0
85	40.0
86	25.0
87	18.0
88	11.0
89	8.0
90	5.0
91	4.0
92	3.0
93	1.5
94	0.0
95	0.5
96	1.0
97	0.5
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	345757.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	48.50362657259579
#Duplication Level	Percentage of deduplicated	Percentage of total
1	68.085942006093	33.02415105906948
2	11.7476812652918	11.396102903711862
3	6.325294118100546	9.203991115985566
4	4.0076196675716	7.7753635120353355
5	2.698614166957871	6.544628690882064
6	1.9854144419151825	5.777988040949562
7	1.358630017127389	4.612893810074642
8	1.0142534623926633	3.9355976951884863
9	0.7511318051845598	3.2789354926924497
>10	2.00572746655478	12.821734994637168
>50	0.014321151135001002	0.4604803237025816
>100	0.004177002414375291	0.34239360335647634
>500	0.0	0.0
>1k	0.0011934292612500832	0.8257387577143085
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CGTTTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1463	0.42312953895365824	No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1390	0.40201644507558776	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.00607362974574629	0.0
2	0.0	0.0	0.0	0.030946589656897762	0.0
3	0.0	0.0	0.0	0.04222618775614087	0.0
4	0.0	0.0	0.0	0.06970213184404075	0.0
5	0.0	0.0	0.0	0.1376689409035826	0.0
6	0.0	0.0	0.0	0.25104336282418	0.0
7	0.0	0.0	0.0	0.3273975653421333	0.0
8	0.0	0.0	0.0	0.8156017087145018	0.0
9	0.0	0.0	0.0	1.0628852055056008	0.0
10	0.0	0.0	0.0	1.4148665102948024	0.0
11	0.0	0.0	0.0	1.5904233319932786	0.0
12	0.0	0.0	0.0	1.770318460653002	0.0
13	0.0	0.0	0.0	1.8472511040991217	0.0
14	0.0	0.0	0.0	1.883114441645433	0.0
15	0.0	0.0	0.0	1.9247621884734076	0.0
16	0.0	0.0	0.0	1.9883906905717021	0.0
17	0.0	0.0	0.0	2.0661909954100715	0.0
18	0.0	0.0	0.0	2.205884479562236	0.0
19	0.0	0.0	0.0	2.259679485881703	0.0
20	0.0	0.0	0.0	2.3293816177257436	0.0
21	0.0	0.0	0.0	2.4100741272049446	0.0
22	0.0	0.0	0.0	2.492212739004561	0.0
23	0.0	0.0	0.0	2.5841848465830046	0.0
24	0.0	0.0	0.0	2.6588037263164592	0.0
25	0.0	0.0	0.0	2.7146232758845086	0.0
26	0.0	0.0	0.0	2.782011644015884	0.0
27	0.0	0.0	0.0	2.84853235075501	0.0
28	0.0	0.0	0.0	2.9086902072843066	0.0
29	0.0	0.0	0.0	2.9890934962994242	0.0
30	0.0	0.0	0.0	3.095815847546109	0.0
31	0.0	0.0	0.0	3.180846663986557	0.0
32	0.0	0.0	0.0	3.263852937178423	0.0
33	0.0	0.0	0.0	3.3514867377956197	0.0
34	0.0	0.0	0.0	3.4434588453740633	0.0
35	0.0	0.0	0.0	3.5490243147644156	0.0
36	0.0	0.0	0.0	3.631162926564032	0.0
37	0.0	0.0	0.0	3.716772183932646	0.0
38	0.0	0.0	0.0	3.8243621965715806	0.0
39	0.0	0.0	0.0	3.980252026712402	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
AGCGATA	25	3.8851285E-5	45.000004	7
AGGCCGA	20	7.026438E-4	45.0	7
AGTACGG	20	7.026438E-4	45.0	2
CGTTTTA	840	0.0	43.660713	1
AGGCGAT	250	0.0	43.2	7
CGTTATT	220	0.0	42.954544	1
CGTTTTT	1220	0.0	42.78688	1
TAGGTAC	75	0.0	42.0	6
ACGGGCT	60	3.6379788E-12	41.249996	5
CAACCCG	55	6.002665E-11	40.909092	23
GTTAGGT	55	6.002665E-11	40.909092	4
GACCCGA	45	1.9215804E-8	40.0	40
GTTTTAT	1000	0.0	39.825	2
GTAAGGT	85	0.0	39.705883	4
GTAAGGC	85	0.0	39.705883	4
GACGTCG	40	3.4488767E-7	39.375	19
TCGCTAT	40	3.4488767E-7	39.375	23
GGCGATA	80	0.0	39.375	8
GTACGGG	75	0.0	39.0	3
GCGACGT	35	6.235623E-6	38.57143	17
>>END_MODULE