FastQCFastQC Report
Thu 26 May 2016
SRR1545700_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1545700_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences1475678
Sequences flagged as poor quality0
Sequence length50
%GC43

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT134050.9083960050905414No Hit
CGTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT58240.39466604503150415No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCGTAA502.7284841E-1144.01
TCGCGAA254.443458E-544.01
CGTTTTA77350.043.658691
CGTTATT42200.043.1137431
GTTTTAT83800.042.0047762
AGGCGAT10400.041.673087
CGACGGT3500.041.48571828
CGGTCTA3450.041.44927631
CACGACG3600.040.94444326
GTTATTT46150.040.663052
CGTTTTT37300.039.871311
TACGGGA3250.039.261544
CGTTTAG800.038.52
GCGCGAC8600.038.116289
TCACGAC3950.037.87341725
CATACGA8100.037.75308618
ACGTGGC8800.037.526
CGTTAAG1000.037.3999982
ATGGGCG3800.037.052635
ACCCGCT8550.037.0526334
Produced by FastQC (version 0.11.5)