##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1545700_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	1475678
Sequences flagged as poor quality	0
Sequence length	50
%GC	43
>>END_MODULE
>>Per base sequence quality	fail
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.46538946843417	31.0	31.0	33.0	30.0	34.0
2	31.65650365459131	31.0	31.0	34.0	30.0	34.0
3	31.629198917378993	31.0	31.0	34.0	30.0	34.0
4	35.580561613034824	37.0	35.0	37.0	33.0	37.0
5	27.17088822900389	35.0	26.0	37.0	0.0	37.0
6	31.359977583185493	35.0	28.0	37.0	17.0	37.0
7	14.931585345854584	0.0	0.0	32.0	0.0	37.0
8	25.05053744787142	17.0	17.0	35.0	17.0	37.0
9	33.11839710289101	32.0	32.0	37.0	28.0	39.0
10	35.92949545903646	37.0	35.0	39.0	32.0	39.0
11	36.93650240770683	38.0	37.0	39.0	34.0	39.0
12	37.131162082784996	39.0	37.0	39.0	34.0	39.0
13	36.82569164817799	39.0	35.0	39.0	33.0	39.0
14	38.2167024242416	40.0	37.0	41.0	33.0	41.0
15	38.42825670640885	40.0	38.0	41.0	34.0	41.0
16	38.41470496951232	40.0	37.0	41.0	34.0	41.0
17	38.41436614220718	40.0	37.0	41.0	34.0	41.0
18	38.365046439670444	40.0	37.0	41.0	34.0	41.0
19	38.285736454700825	40.0	37.0	41.0	34.0	41.0
20	38.284725394022274	40.0	37.0	41.0	34.0	41.0
21	38.14527898362651	40.0	37.0	41.0	34.0	41.0
22	37.98877736199903	40.0	36.0	41.0	34.0	41.0
23	37.821817496770976	39.0	36.0	41.0	33.0	41.0
24	37.63659822806873	39.0	35.0	41.0	33.0	41.0
25	37.45117295236495	39.0	35.0	41.0	33.0	41.0
26	37.18993710009907	39.0	35.0	40.0	33.0	41.0
27	36.98035343753854	39.0	35.0	40.0	32.0	41.0
28	36.97530220007346	39.0	35.0	40.0	32.0	41.0
29	36.86449821709072	39.0	35.0	40.0	32.0	41.0
30	36.59509391615244	38.0	35.0	40.0	31.0	41.0
31	36.35886826258845	38.0	35.0	40.0	31.0	41.0
32	36.0366767004726	38.0	35.0	40.0	30.0	41.0
33	35.68036726169259	38.0	35.0	40.0	28.0	41.0
34	35.26817232485678	38.0	35.0	40.0	25.0	41.0
35	34.96368720005313	38.0	34.0	40.0	23.0	41.0
36	34.72122509111066	38.0	34.0	40.0	22.0	41.0
37	34.5812650185203	38.0	34.0	40.0	22.0	41.0
38	34.4006863285893	38.0	34.0	40.0	21.0	41.0
39	34.28454242727749	38.0	34.0	40.0	21.0	41.0
40	34.0657785777114	38.0	33.0	40.0	20.0	41.0
41	33.836001485418905	37.0	33.0	40.0	18.0	40.0
42	33.8239399110104	37.0	33.0	40.0	18.0	40.0
43	33.70791324394617	37.0	33.0	40.0	18.0	40.0
44	33.46959973652789	37.0	33.0	40.0	18.0	40.0
45	33.289067804765	37.0	33.0	39.0	18.0	40.0
46	33.21511671245353	36.0	33.0	39.0	18.0	40.0
47	33.0333372185531	36.0	32.0	39.0	18.0	40.0
48	32.938329364536166	36.0	32.0	39.0	18.0	40.0
49	32.76382923645944	36.0	32.0	39.0	17.0	40.0
50	32.891676232890916	36.0	32.0	39.0	18.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
9	1.0
10	1.0
11	3.0
12	9.0
13	7.0
14	14.0
15	41.0
16	91.0
17	308.0
18	742.0
19	1512.0
20	2907.0
21	4938.0
22	7934.0
23	12425.0
24	18940.0
25	27931.0
26	33990.0
27	33412.0
28	31877.0
29	32017.0
30	36088.0
31	45479.0
32	60938.0
33	99152.0
34	112941.0
35	148199.0
36	208496.0
37	364680.0
38	150119.0
39	40486.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	32.44020714546127	21.971595429355183	24.724973876414776	20.86322354876877
2	37.9263633394277	23.27580949231472	27.042891470903545	11.75493569735403
3	28.741093924284293	23.041679824460353	36.41397378018782	11.803252471067536
4	25.06813817106442	26.09464937472809	34.888776548813496	13.948435905393994
5	16.719704434165177	46.81854713562173	25.95044447365889	10.511303956554208
6	18.829582063295653	38.67164787982202	32.6754210606921	9.823348996190227
7	36.28169560025968	55.91287530206455	6.783932538128237	1.0214965595475436
8	81.22856070226703	3.3161706009034493	13.635766068207293	1.8195026286222333
9	76.72276743300368	5.096775854895173	15.149985294894957	3.0304714172061926
10	39.144921859646885	26.739234440033666	21.84216339879025	12.273680301529195
11	32.912193581526594	22.773870722474687	29.323402530904442	14.990533165094282
12	30.679389406089946	20.93322527001148	33.10430866354313	15.283076660355444
13	21.683931047288095	30.10121449259256	31.779832727735997	16.43502173238335
14	17.407523863607103	31.44324168280614	33.92481286567937	17.22442158790739
15	16.436648103448043	23.42624881579857	42.73445833034036	17.402644750413028
16	18.41140140328717	20.188957211532596	41.20892227166089	20.190719113519346
17	18.421837284285598	20.726066255646558	34.697339121407246	26.154757338660602
18	20.998551174443207	22.84441456740563	36.32099956765636	19.83603469049481
19	25.16924423891933	25.015348876923017	30.380001599264876	19.435405284892777
20	26.505511364945466	23.03456445105233	31.521714086677445	18.938210097324756
21	22.13477465951244	25.464837179926786	33.02258351754245	19.37780464301833
22	22.34477982324057	23.39378915996579	31.261765778171117	22.99966523862252
23	20.069757765583006	26.446487648389414	31.858169600685244	21.625584985342332
24	19.628130256058572	23.57824674488608	38.802774046912674	17.990848952142677
25	19.846063978727067	25.035068626082385	34.91547614045883	20.203391254731724
26	18.949120336550386	28.106199319905834	31.901878323048795	21.042802020494985
27	18.469679699771902	27.53988336208848	33.27074063582977	20.719696302309853
28	18.25364341001221	25.8168787499712	36.79969478436353	19.129783055653064
29	18.372097435890485	23.617754008665848	36.8564144752446	21.15373408019907
30	19.68728950353668	27.0731826319834	34.16246633750723	19.077061526972685
31	23.92554473265848	26.536751242479728	30.56825405000278	18.969449974859014
32	24.823437091289563	25.260863142230217	30.313591447456695	19.602108319023525
33	23.987075771272597	26.757260052667313	29.446464608132665	19.809199567927422
34	20.131695396963295	26.57571638257126	31.683605773075158	21.608982447390286
35	21.230241285700536	26.298420116041576	31.375340690855325	21.095997907402563
36	24.714470229955314	27.951084179610998	27.70380801231705	19.630637578116637
37	21.262768706994343	30.32626358866907	29.03079127018225	19.380176434154333
38	21.60769490363074	29.128847892290864	29.138606118679007	20.12485108539939
39	21.425270282541316	29.887143401202703	27.724815305235968	20.96277101102002
40	23.560017835869342	26.514930764028467	28.904205388980525	21.02084601112167
41	20.51091091688024	25.660001707689617	29.726810320408653	24.10227705502149
42	21.030943064814952	27.770150398664207	27.21298277808573	23.985923758435106
43	21.457458876529977	27.368030152919538	28.13377986254454	23.040731108005946
44	21.052831308727242	28.044668281291717	29.34949223340051	21.553008176580526
45	19.452346650149966	31.6789977217252	26.891842258270437	21.9768133698544
46	21.622061181368835	29.812533628610034	28.196395148535114	20.369010041486018
47	20.716782387485615	28.462713410378143	29.426880389895356	21.393623812240882
48	21.402636618557707	26.132665798365224	30.72303036299247	21.741667220084597
49	21.35648833959712	26.653985490059483	30.342052941088777	21.647473229254622
50	19.770708786063086	28.703822920718476	30.19859346009089	21.32687483312755
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	1203.0
1	1918.5
2	2634.0
3	20956.0
4	39278.0
5	27976.5
6	16675.0
7	16589.5
8	16504.0
9	16357.0
10	16210.0
11	16165.5
12	16121.0
13	15388.5
14	14656.0
15	13968.0
16	13280.0
17	12533.0
18	11786.0
19	11404.5
20	11023.0
21	10741.5
22	10460.0
23	10901.5
24	11343.0
25	13011.0
26	14679.0
27	16939.0
28	19199.0
29	22251.5
30	25304.0
31	29723.0
32	34142.0
33	38620.0
34	43098.0
35	46307.0
36	49516.0
37	54578.0
38	59640.0
39	69492.0
40	79344.0
41	89021.5
42	98699.0
43	101361.5
44	104024.0
45	105875.5
46	107727.0
47	107508.0
48	107289.0
49	102534.0
50	97779.0
51	90132.5
52	82486.0
53	75693.5
54	68901.0
55	66011.5
56	63122.0
57	60671.5
58	58221.0
59	54624.5
60	51028.0
61	44981.0
62	38934.0
63	33655.0
64	28376.0
65	24222.0
66	20068.0
67	17358.5
68	14649.0
69	12873.5
70	11098.0
71	9299.0
72	7500.0
73	5984.0
74	4468.0
75	3544.0
76	2620.0
77	2014.0
78	1408.0
79	1024.5
80	641.0
81	464.5
82	288.0
83	229.5
84	171.0
85	112.0
86	53.0
87	34.5
88	16.0
89	13.5
90	11.0
91	6.0
92	1.0
93	3.0
94	5.0
95	2.5
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
50	1475678.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	33.99057417791288
#Duplication Level	Percentage of deduplicated	Percentage of total
1	71.5067652222616	24.305560075098846
2	8.245399424962994	5.605317215614498
3	4.321426511958703	4.406633052273949
4	2.800647592526992	3.807824789599274
5	2.0918842229773795	3.5552172926359136
6	1.555129352845185	3.1715843774480357
7	1.229894596382625	2.9263376456550487
8	0.9931400759029874	2.700592113523082
9	0.8387167352552584	2.5657617063555707
>10	6.225063334587289	40.330591226347074
>50	0.16697788291925303	3.4945883178426467
>100	0.023743637401868398	1.3281075816452137
>500	4.8456400716895753E-4	0.12418839057535863
>1k	2.4228200358447876E-4	0.09412952946129637
>5k	2.4228200358447876E-4	0.4796241291186265
>10k+	2.4228200358447876E-4	1.1039425568054924
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CGTTTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	13405	0.9083960050905414	No Hit
CGTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	5824	0.39466604503150415	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.009825991849170348	0.0
2	0.0	0.0	0.0	0.034086026897466794	0.0
3	0.0	0.0	0.0	0.05068856484951324	0.0
4	0.0	0.0	0.0	0.06654568273024332	0.0
5	0.0	0.0	0.0	0.10971228140556409	0.0
6	0.0	0.0	0.0	0.21996668649935827	0.0
7	0.0	0.0	0.0	0.2922046679560175	0.0
8	0.0	0.0	0.0	0.6173433499720129	0.0
9	0.0	0.0	0.0	0.8074932336187163	0.0
10	0.0	0.0	0.0	1.080384745181537	0.0
11	0.0	0.0	0.0	1.274329494645851	0.0
12	0.0	0.0	0.0	1.456618584813218	0.0
13	0.0	0.0	0.0	1.5455946351439813	0.0
14	0.0	0.0	0.0	1.5888289992803308	0.0
15	0.0	0.0	0.0	1.6371457729938375	0.0
16	0.0	0.0	0.0	1.7289679726878087	0.0
17	0.0	0.0	0.0	1.8342077336654745	0.0
18	0.0	0.0	0.0	2.00016534772491	0.0
19	0.0	0.0	0.0	2.070573661733793	0.0
20	0.0	0.0	0.0	2.1480973491506954	0.0
21	0.0	0.0	0.0	2.2351082011116246	0.0
22	0.0	0.0	0.0	2.3329615268371557	0.0
23	0.0	0.0	0.0	2.4458587849110716	0.0
24	0.0	0.0	0.0	2.532191982261713	0.0
25	0.0	0.0	0.0	2.6046332601014583	0.0
26	0.0	0.0	0.0	2.6775488961684055	0.0
27	0.0	0.0	0.0	2.755682472734567	0.0
28	0.0	0.0	0.0	2.8315120236257503	0.0
29	0.0	0.0	0.0	2.9202170121123987	0.0
30	0.0	0.0	0.0	3.0368413705428963	0.0
31	0.0	0.0	0.0	3.1294089903081836	0.0
32	0.0	0.0	0.0	3.2230608574499313	0.0
33	0.0	0.0	0.0	3.314408698916701	0.0
34	6.776546102876101E-5	0.0	0.0	3.4046045275459824	0.0
35	6.776546102876101E-5	0.0	0.0	3.5181794402301856	0.0
36	6.776546102876101E-5	0.0	0.0	3.612780023826336	0.0
37	6.776546102876101E-5	0.0	0.0	3.7151058699797654	0.0
38	6.776546102876101E-5	0.0	0.0	3.8187870253537697	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
AGCGTAA	50	2.7284841E-11	44.0	1
TCGCGAA	25	4.443458E-5	44.0	1
CGTTTTA	7735	0.0	43.65869	1
CGTTATT	4220	0.0	43.113743	1
GTTTTAT	8380	0.0	42.004776	2
AGGCGAT	1040	0.0	41.67308	7
CGACGGT	350	0.0	41.485718	28
CGGTCTA	345	0.0	41.449276	31
CACGACG	360	0.0	40.944443	26
GTTATTT	4615	0.0	40.66305	2
CGTTTTT	3730	0.0	39.87131	1
TACGGGA	325	0.0	39.26154	4
CGTTTAG	80	0.0	38.5	2
GCGCGAC	860	0.0	38.11628	9
TCACGAC	395	0.0	37.873417	25
CATACGA	810	0.0	37.753086	18
ACGTGGC	880	0.0	37.5	26
CGTTAAG	100	0.0	37.399998	2
ATGGGCG	380	0.0	37.05263	5
ACCCGCT	855	0.0	37.05263	34
>>END_MODULE