FastQCFastQC Report
Thu 26 May 2016
SRR1545699_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1545699_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences558836
Sequences flagged as poor quality0
Sequence length51
%GC43

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT35420.6338174348109284No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT32940.589439477771654No Hit
CGTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT6340.11345009985040334No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AACTTCG302.1634914E-645.00000411
TCGTAAG302.1634914E-645.0000042
TTTACGG207.030171E-445.02
TCGATAG351.2104283E-745.02
ATCGTAG207.030171E-445.02
TGCGAAA253.888225E-545.01
CGTAAAG207.030171E-445.02
ATGCGAA207.030171E-445.01
CGCGTAA253.888225E-545.031
GCGTAAC253.888225E-545.032
ACGACAG207.030171E-445.02
CGTTTTA20200.044.443071
CGTTTTT26350.043.6337781
TCTCACG1150.043.0434823
CGGTCTA1100.042.95454831
ATAGGCC1050.042.8571475
AGGCGAT4000.042.18757
GTTTTAT22600.041.6150442
CGTTATT4400.040.3977281
GCGCGAC3300.040.2272729